Recombinant human epidermal growth factor combined with vacuum sealing drainage for wound healing in Bama pigs

Abstract

Background: Vacuum sealing drainage (VSD) and epidermal growth factor (EGF) both play an important role in the treatment of wounds. This study aims to explore the effect of the combination of VSD and EGF on wound healing and the optimal concentration and time of EGF.Methods: In this study, we tested the proliferation and migration capacity of HaCaT and L929 cells at different EGF concentrations (0, 1, 5, 10, and 100 ng/ml) and different EGF action times (2, 10, and 30min). A full-thickness skin defect model was established using male, 30-week-old Bama pigs. The skin defects were randomly divided follows: routine dressing change after covering with sterile auxiliary material (Control), continuous negative pressure drainage of the wound (VSD), continuous negative pressure drainage of the wound and injection of epidermal growth factor 10 minutes followed by removal by continuous lavage (V+E 10 min), and continuous negative pressure drainage of the wound and injection of epidermal growth factor 30 minutes followed by removal by continuous lavage (V+E 30 min). The wound healing rate, histological repair effect and collagen deposition were compared among the four groups.Results: An EGF concentration of 10 ng/ml and an action time of 10 minutes had optimal effects on the proliferation and migration capacities of HaCaT and L929 cells. The drug dispersion effect was better than drug infusion after bolus injection effect, and the contact surface was wider. Compared with other groups, the V+E 10min group promoted wound healing to the greatest extent and obtained the best histological score. Conclusions: A rhEGF concentration of 10 ng/ml can promote the proliferation and migration of epithelial cells and fibroblasts to the greatest extent in vitro. VSD combined with rhEGF kept in place for 10 minutes and then washed, can promote wound healing better than the other treatments in vivo.

Effects of Recombinant Human Epidermal Growth Factor Eye Drops Combined with Phacoemulsification on Short- and Long-Term Visual Acuity Recovery and Related Dry Eye Complications in Patients with Senile Cataract

“…Recombinant human epidermal growth factor (RhEGF) is a kind of cell growth factor composed of many kinds of amino acids, and RhEGF eye drops can effectively promote cell division and receptor binding to a certain extent, activate cells, cause cell cascade reaction, increase protein, RNA, and DNA synthesis, accelerate cell metabolism, promote the proliferation of corneal epidermal cells, and accelerate the proliferation and differentiation of corneal epithelial stem cells [6]. In addition, it can stimulate the proliferation of fibrous cells and corneal stroma and enhance the arrangement of collagen lamella [6,7]. Optogenetics is a technique that combines optics and genetics to control specific neurons, which is widely applied in the peripheral nervous system, such as vision study [7].…”

Efficacy of Human-Recombinant Epidermal Growth Factor Combined with Povidone-Iodine for Pressure Ulcers and Its Influence on Inflammatory Cytokines

“…Recent research shows that a growing number of novel technologies, such as recombinant human epidermal growth factor (rh-EGF) and povidone iodine (PVI), contribute to accelerated wound healing [6,7]. EGF is shown to promote skin repair and remodeling by stimulating the growth of epithelial cells in wounds, thus speeding up wound healing [8,9].…”

Pushing the frontiers of military medical excellence: updates, progress and future needs

“…A few authors investigated the effect of novel methods for wound healing in animal studies. Wei et al [47] studied Bama pigs and discovered that recombinant human epidermal growth factor at a concentration of 10 ng/ml can promote the proliferation and migration of epithelial cells and fibroblasts to the greatest extent, and can be further enhanced by vacuum sealing drainage. Yang et al [48] improved the use of LL-37 peptide to promote wound healing by incorporating it into chitosan hydrogel, improving its stability in the wound environment, thus improving the healing of pressure ulcers in a mouse model.…”