Recombinant human epidermal growth factor (REGEN-D™ 150): Effect on healing of diabetic foot ulcers

Mohan, V.

doi:10.1016/j.diabres.2007.06.004

Cited by 65 publications

(49 citation statements)

References 4 publications

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“…Diğer bir büyüme faktörü olan epidermal büyüme faktö-rü (EGF)'nün topikal uygulamayla DA yaralarında kapanma oranını artırdığı (152)(153)(154), kapanma süresini kısalttığı ve gü-venli bir uygulama olduğu ortaya konmuştur (155,156). İntra-lezyonel olarak uygulanan EGF'nin özellikle iskemik karakterli DA yaralarında yara iyileşme oranlarını artırdığı, iyileşme sü-resini kısalttığı ve ampütasyon riskini azalttığı, ürünün üre-tildiği ülkede yapılan bir dizi çalışmayla gösterilmiştir (157)(158)(159)(160)(161).…”

Section: Soru 7: Ozon Uygulamasının Diyabetik Ayak Yaralarının Tedaviunclassified

Diagnosis, Treatment and Prevention of Diabetic Foot Wounds and Infections: Turkish Consensus Report

Saltoğlu¹,

Kılıçoğlu²,

Baktiroğlu³

et al. 2016

Klimik Dergisi

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Study Group for Diabetic Foot Infections of the Turkish Society of Clinical Microbiology and Infectious Diseases has called for collaboration of the relevant specialist societies and the Ministry of Health to issue a national consensus report on the diagnosis, treatment and prevention of diabetic foot (DF) wounds and diabetic foot infections (DFIs) in Turkey. In the periodical meetings of the assigned representatives from all the parties, various questions as to pathogenesis, microbiology, assessment and grading, treatment, prevention and control of diabetic foot were identified. Upon reviewing related literature and international guidelines, these questions were provided

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Section: Soru 7: Ozon Uygulamasının Diyabetik Ayak Yaralarının Tedaviunclassified

Diagnosis, Treatment and Prevention of Diabetic Foot Wounds and Infections: Turkish Consensus Report

Saltoğlu¹,

Kılıçoğlu²,

Baktiroğlu³

et al. 2016

Klimik Dergisi

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“…Intriguingly, however, even in the presence of TGF-β3 ( Figure 7A, far right column), F-5 remained equally effective on stimulation of migration of all 3 cell types. Quantitation of these results is shown in Figure 7B (bars [10][11][12][13][14][15]. This second unique property of F-5 provides another explanation for why F-5 heals wounds faster than becaplermin.…”

Section: F-5 Is Superior To Becaplermin Gel/pdgf-bb In Promoting Diabmentioning

confidence: 99%

“…Since the first report of the EGF clinical trial on wound healing in 1989 (8), more than a dozen growth factor trials have been conducted. The list includes (a) EGF on partialthickness wounds of skin grafts (8), on traumatic corneal epithelial defects (9), on tympanic membrane with chronic perforation (10), and on advanced diabetic foot ulcers (11,12); (b) bFGF on partialthickness burn wounds of children (13), on second-degree burns (14), and on diabetic ulcers (15); (c) acidic FGF on partial-thickness burns and skin graft donor sites (16); (d) GM-CSF plus bFGF on pressure ulcers (17); and (e) PDGF-BB on chronic pressure and diabetic ulcers (18)(19)(20)(21)(22). Despite the fact that most of these doubleblinded trials reported promising clinical efficacies in humans, only the human recombinant PDGF-BB has received US FDA approval for treatment of limb diabetic ulcers (Regranex, becaplermin gel 0.01%, Ortho-McNeil Pharmaceutical) (20).…”

Section: Introductionmentioning

confidence: 99%

A fragment of secreted Hsp90α carries properties that enable it to accelerate effectively both acute and diabetic wound healing in mice

Cheng

Sahu²,

Tsen³

et al. 2011

J. Clin. Invest.

116

106

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Wounds that fail to heal in a timely manner, for example, diabetic foot ulcers, pose a health, economic, and social problem worldwide. For decades, conventional wisdom has pointed to growth factors as the main driving force of wound healing; thus, growth factors have become the center of therapeutic developments. To date, becaplermin (recombinant human PDGF-BB) is the only US FDA-approved growth factor therapy, and it shows modest efficacy, is costly, and has the potential to cause cancer in patients. Other molecules that drive wound healing have therefore been sought. In this context, it has been noticed that wounds do not heal without the participation of secreted Hsp90α. Here, we report that a 115-aa fragment of secreted Hsp90α (F-5) acts as an unconventional wound healing agent in mice. Topical application of F-5 peptide promoted acute and diabetic wound closure in mice far more effectively than did PDGF-BB. The stronger effect of F-5 was due to 3 properties not held by conventional growth factors: its ability to recruit both epidermal and dermal cells; the fact that its ability to promote dermal cell migration was not inhibited by TGF-β; and its ability to override the inhibitory effects of hyperglycemia on cell migration in diabetes. The discovery of F-5 challenges the long-standing paradigm of wound healing factors and reveals a potentially more effective and safer agent for healing acute and diabetic wounds.

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“…3,6 All of these may be caused by lack of growth factor supplies 7,8 as they are indicated by the decrease of GF-gene expressions among diabetic patients who tend to have chronic ulcers, 9 the entrapment of GFs by fibrin that spill out when capillaries leak due to high venous pressure in patients with venous leg ulcers, 7,10,11 and GFs degradation by protease contained in bio-film on the surface of chronic ulcer. 2,12 The theory of GFs supply deficiency will be strengthened when considering the findings of the clinicians who succeeded in stimulating chronic ulcers healing using either recombinant platelet derived growth factor (r-PDGF), 13 or recombinant epidermal growth factor (r-EGF), [14][15][16] or recombinant fibroblast growth factor (r-FGF) that were applied topically.…”

Section: Introductionmentioning

confidence: 99%

The Effect of PRF on Serum Starved Human Dermal Fibroblast

2016

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Healing failure on chronic ulcers was suspected due to the decrease of Growth Factors (GFs) supply caused by either GFs trapped in the fibrin, or degraded by protease, or decreased level due to reduction of GFs gene expression. Administration of various GFs can stimulate healing of chronic ulcers. High level of GFs is available in biologic material called Platelet Rich Fibrin (PRF). This study was conducted to have in vitro evidence of PRF effect on GFs-serum starved human dermal fibroblasts as representative cells of chronic ulcers. Human dermal fibroblasts (HDFs) were isolated from foreskin of six boys aged 11-14 years-old. After 24 hours of serum deprivation, HDFs were treated by 100, 50, and 25% PRF lysate diluted in cultured medium. Cellular migration was measured using scratch assay, while cellular viability was measured using MTT assay and collagen deposition was measured using Sirius Red assay. The HDFs of serum starvation group showed significant impairment activities in terms of cellular migration (25%), cellular proliferation (20%), and collagen deposition (10%) (p<0.05). Administration with various levels of PRF lysate could significantly recover those activities (p < 0.05). Because cellular activities of serum starved HDFs is similar with fibroblasts isolated from the bottom of chronic ulcers and administration of various levels of PRF lysate was capable to recover those activities, it can be concluded that PRF is a good biologic material to stimulate healing of chronic ulcers. However, in order to get better evidence based medicine, both pre clinical and clinical studies must be performed. ABSTRAKKegagalan sembuh pada ulkus khronis disebabkan oleh rendahnya suplai growth factor (GF) akibat GF terjebak dalam fibrin, atau dirusak oleh protease bakteri, atau akibat menurunnya ekspresi gena penyandi GF. Pemberian GF dapat memacu penyembuhan ulkus khronis. Berbagai GF ternyata terdapat dalam material biologis seperti fibrin kaya platelet (FKP). Penelitian ini dilakukan untuk memperoleh bukti in vitro bahwa FKP dapat mengembalikan fungsi fibroblast tanpa serum sebagai model fibroblas dari ulkus khronis. Fibroblas dermis manusia (FDM) diisolasi dari kulit kulup 6 anak laki-laki berumur 11-14 tahun. Setelah dibebaskan dari serum selama 24 jam, FDM kemudian diberi 100, 50, 25% lisat FKP dan medium pelarut sebagai kontrol. Selanjutnya, aktivitas selular berupa timbunan kolagen, proliferasi dan migrasi sel diukur berturut-turut dengan metode Sirius Merah, MTT, dan goresan. FDM tanpa serum ternyata secara mengalami penurunan kemampuan menimbun kolagen sebesar 10%, proliferasi sebesar 20% dan penurunan kemampuan migrasi sebesar 25% secara signifikan (p<0,05). Penambahan lisat FKP 111Radiono, The effect of platelet rich fibrin (PRF) on serum starved human dermal fibroblast ternyata dapat memulihkan aktivitas selular FDM tersebut. Dari eksperimen ini dapat disimpulkan bahwa FKP merupakan material biologis yang dapat dikembangkan untuk memacu penyembuhan ulkus khronis. Namun demikian, untuk memperoleh bukt...

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Recombinant human epidermal growth factor (REGEN-D™ 150): Effect on healing of diabetic foot ulcers

Cited by 65 publications

References 4 publications

Diagnosis, Treatment and Prevention of Diabetic Foot Wounds and Infections: Turkish Consensus Report

Diagnosis, Treatment and Prevention of Diabetic Foot Wounds and Infections: Turkish Consensus Report

A fragment of secreted Hsp90α carries properties that enable it to accelerate effectively both acute and diabetic wound healing in mice

The Effect of PRF on Serum Starved Human Dermal Fibroblast

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