Recombinant human interleukin 5 is a selective eosinophil chemoattractant

Wang, Ji Ming; Rambaldi, Alessandro; Biondi, Andrea; Chen, Zhen Guo; Sanderson, Colin J.; Mantovani, Alberto

doi:10.1002/eji.1830190420

Cited by 294 publications

(141 citation statements)

References 22 publications

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“…In this regard, a number of studies have demonstrated increased concentrations of various cytokines in the bronchoalveolar lavage fluid isolated from allergic asthmatic individuals (8)(9)(10). While the precise role of each cytokine remains to be established, collectively, these agents have been found to exert a diversity of actions including induction of specific IgE production by mononuclear cells (11)(12), antigen presentation by macrophages (13), activation of airway structural cells (5,(14)(15)(16), altered expression of adhesion molecules (17,18), induced secretion of various growth factors (19,20), recruitment of inflammatory cells from the circulation (21)(22)(23)(24), and other proinflammatory events.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of cytokine-induced modulation of beta-adrenoceptor responsiveness in airway smooth muscle.

Hákonarson

Herrick²,

Serrano³

et al. 1996

J. Clin. Invest.

128

112

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To elucidate the role of specific proinflammatory cytokines in regulating airway responsiveness, we examined the effects and mechanisms of action of IL-1 ␤ , TNF-␣ , and IL-2 on the ␤ -adrenoceptor-and postreceptor-coupled transmembrane signaling mechanisms regulating relaxation in isolated rabbit tracheal smooth muscle (TSM) segments. During half-maximal isometric contraction of the tissues with acetylcholine, relaxation responses to isoproterenol, PGE 2 , and forskolin were separately compared in control (untreated) TSM and tissues incubated for 18 h with IL-1 ␤ (10 ng/ml), TNF-␣ (100 ng/ml), or IL-2 (200 ng/ml). Relative to controls, IL-1 ␤ -and TNF-␣ -treated TSM, but not IL-2-treated tissues, depicted significant attenuation of their maximal relaxation and sensitivity (i.e., Ϫ log dose producing 50% maximal relaxation) to isoproterenol ( P Ͻ 0.001) and PGE 2 ( P Ͻ 0.05); whereas the relaxation responses to direct stimulation of adenylate cyclase with forskolin were similar in the control and cytokine-treated tissues. Further, the attenuated relaxation to isoproterenol and PGE 2 was ablated in the IL-1 ␤ -treated TSM that were pretreated with either the muscarinic M 2 -receptor antagonist, methoctramine (10

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Section: Introductionmentioning

confidence: 99%

Mechanism of cytokine-induced modulation of beta-adrenoceptor responsiveness in airway smooth muscle.

Hákonarson

Herrick²,

Serrano³

et al. 1996

J. Clin. Invest.

128

112

View full text Add to dashboard Cite

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“…Several cytokines, including IL-3, IL-5, and GM-CSF are reported to regulate the development of eosinophils from hematopoietic stem cells and to support their survival in vitro (18 -21). IL-5 is one of the most important regulators of many eosinophil functions including chemotaxis (22,23), degranulation (24,25), adhesion (26), and cytotoxicity for parasites (27). Moreover, treatment of normal eosinophils with IL-5, IL-3, or GM-CSF enhanced their cysLT production in vitro (28 -30).…”

mentioning

confidence: 99%

IL-5 Up-Regulates Cysteinyl Leukotriene 1 Receptor Expression in HL-60 Cells Differentiated into Eosinophils

Thivierge

Doty

Johnson

et al. 2000

The Journal of Immunology

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The cysteinyl leukotrienes, leukotriene (LT) C4, LTD4, and LTE4, are lipid mediators that have been implicated in the pathogenesis of several inflammatory processes, including asthma. The human LTD4 receptor, CysLT1R, was recently cloned and characterized. We had previously shown that HL-60 cells differentiated toward the eosinophilic lineage (HL-60/eos) developed specific functional LTD4 receptors. The present work was undertaken to study the potential modulation of CysLT1R expression in HL-60/eos by IL-5, an important regulator of eosinophil function. Here, we report that IL-5 rapidly up-regulates CysLT1R mRNA expression, with consequently enhanced CysLT1R protein expression and function in HL-60/eos. CysLT1R mRNA expression was augmented 2- to 15-fold following treatment with IL-5 (1–20 ng/ml). The effect was seen after 2 h, was maximal by 4 h, and maintained at 8 h. Although CysLT1R mRNA was constitutively expressed in undifferentiated HL-60 cells, its expression was not modulated by IL-5 in the absence of differentiation. Differentiated HL-60/eos cells pretreated with IL-5 (10 ng/ml) for 24 h showed enhanced CysLT1R expression on the cell surface, as assessed by flow cytometry using a polyclonal anti-CysLT1R Ab. They also showed enhanced responsiveness to LTD4, but not to LTB4 or platelet-activating factor, in terms of Ca2+ mobilization, and augmented the chemotactic response to LTD4. Our findings suggest a possible mechanism by which IL-5 can modulate eosinophil functions and particularly their responsiveness to LTD4, and thus contribute to the pathogenesis of asthma and allergic diseases.

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“…IL-5 is the predominant eosinophilopoietin produced by cloned T cells of hypereosinophilic patients. 17,18 IL-5 also functions as a chemoattractant for eosinophils 19 but is not known to possess neurotoxic or encephalopathic activity. Increased susceptibility to intracranial dissemination of Angiostrongylus cantonensis in IL-5␣-deficient mice has been reported, reinforcing the importance of IL-5 in the induction of eosinophilassociated killing of invasive helminths.…”

Section: Discussionmentioning

confidence: 99%

Eosinophil-Associated Inflammation and Elaboration of Eosinophil-Derived Proteins in 2 Children With Raccoon Roundworm (Baylisascaris procyonis)Encephalitis

Moertel

Kazacos

Butterfield³

et al. 2001

Pediatrics

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ABSTRACT. Objective. Eosinophil-associated proteins, especially eosinophil-derived neurotoxin, may be important contributors to the neurologic pathology and symptoms caused by Baylisascaris procyonis infection.Methods. Two cases of severe B procyonis encephalitis with evidence of marked eosinophil degranulation in the central nervous system are presented. Serial cerebrospinal fluid (CSF) specimens were collected from each patient during the course of their illness. Antibodies against B procyonis were measured in the patients' serum and CSF. Levels of the eosinophilopoietin interleukin-5 (IL-5) and 2 important eosinophil proteins, eosinophilderived neurotoxin and major basic protein, were assayed in the CSF.Results. Both patients had rapidly progressive central nervous system disease with evidence of eosinophilic meningoencephalitis. Both tested positive for antibodies to B procyonis in serum and CSF and had progressively worsening deep white matter changes on magnetic resonance images of the brain. CSF levels of IL-5, eosinophilderived neurotoxin, and major basic protein were markedly elevated over controls.Conclusions. This is the first report of the measurement of IL-5, eosinophil-derived neurotoxin, and major basic protein in human CSF. In addition to traumatic damage and necrosis caused by migrating larvae, eosinophil-derived neurotoxin from associated eosinophilic inflammation may be an important contributory factor in the pathogenesis of B procyonis encephalitis. Pediatrics 2001;108(5). URL: http://www.pediatrics.org/cgi/content/ full/108/5/e93; parasite, eosinophil-derived-neurotoxin, major basic protein, eosinophilia, hypereosinophilia, interleukin-5, encephalitis, child.ABBREVIATIONS. CSF, cerebrospinal fluid; CNS, central nervous system; MRI, magnetic resonance imaging; IL-5, interleukin-5; PBS, phosphate-buffered saline.B aylisascaris procyonis, the common raccoon ascarid, is a well-known cause of severe neurologic disease (neural larva migrans) in animals, with cases identified in Ͼ90 species of mammals and birds in North America. 1,2 Neural larva migrans and eosinophilic meningoencephalitis attributable to B procyonis have now been identified in 7 young children, including the 2 reported here. [3][4][5][6][7] We specifically note a novel correlation with elevated cerebrospinal fluid (CSF) levels of major basic protein and the neurotoxic protein eosinophil-derived neurotoxin associated with eosinophilic inflammation in these 2 cases. We hypothesize that the severe neurologic consequences of Baylisascaris encephalitis are attributable, in part, to marked eosinophil degranulation and release of these toxic eosinophil proteins in the central nervous system (CNS) of patients with this disease. CASE REPORTS Case 1A 13-month-old boy became irritable 3 weeks before hospital admission, then became progressively irritable, ataxic, and weak. Three days before admission, he was unable to cruise, sit up, or walk, and exhibited dysmetria. He then had an episode of limb twitching followed by rigidity and decreased...

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Recombinant human interleukin 5 is a selective eosinophil chemoattractant

Cited by 294 publications

References 22 publications

Mechanism of cytokine-induced modulation of beta-adrenoceptor responsiveness in airway smooth muscle.

Mechanism of cytokine-induced modulation of beta-adrenoceptor responsiveness in airway smooth muscle.

IL-5 Up-Regulates Cysteinyl Leukotriene 1 Receptor Expression in HL-60 Cells Differentiated into Eosinophils

Eosinophil-Associated Inflammation and Elaboration of Eosinophil-Derived Proteins in 2 Children With Raccoon Roundworm (Baylisascaris procyonis)Encephalitis

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