Recombinant LipL32 antigen-based single serum dilution ELISA for detection of canine leptospirosis

Dey, Sohini; Mohan, C. Madhan; Kumar, Tarun; Ramadass, P.; Nainar, A. Mahalinga; Nachimuthu, K.

doi:10.1016/j.vetmic.2004.07.018

Cited by 71 publications

(44 citation statements)

References 15 publications

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“…The sensitivity and specificity of ELISA relative to MAT were 8.6% and 96.2%, respectively (Table 3). A study of 137 serum samples using ELISA that detects Leptospira antibodies in dogs, with recombinant LipL32 protein as the antigen, found the sensitivity and specificity relative to MAT with seven standard serovars (Icterohaemorrhagiae, Canicola, Autumnalis, Hebdomadis, Pomona, Grippotyphosa, and Javanica) as the antigens to be 96.9% and 97.3%, respectively [6]. The specificity was similar in the present study, but the sensitivity was markedly lower.…”

Section: Discussionsupporting

confidence: 70%

Nationwide Survey of Leptospira Antibodies in Dogs in Japan: Results from Microscopic Agglutination Test and Enzyme-Linked Immunosorbent Assay

Iwamoto

Wada

Fujisaki

et al. 2009

The Journal of Veterinary Medical Science

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ABSTRACT. Leptospirosis is an infectious disease caused by Leptospira interrogans sensu lato and is common in both humans and animals. In the present study, serum samples were collected from 801 dogs across all 47 prefectures in Japan, and evaluated with a microscopic agglutination test (MAT), using 5 major L. interrogans serovars (Icterohaemorrhagiae, Canicola, Autumnalis, Hebdomadis, and Australis) as antigens, and an enzyme-linked immunosorbent assay (ELISA) using recombinant OmpL1 protein as the antigen. Across all dogs tested, 217 (27.0%) and 29 (3.6%) were MAT-and ELISA-positive, respectively. However, evidence strongly suggests that MAT also detected antibodies produced by vaccination. Of 243 dogs never inoculated with any canine vaccine, 41 (16.9%) from 23 prefectures were MAT and/or ELISA positive. The most commonly detected serovar was Icterohaemorrhagiae (22 dogs, 19 prefectures). Our results suggest that there are dogs with subclinical Leptospira infection throughout Japan. To the best of our knowledge, the present study is the first nationwide survey of Leptospira infection in dogs, and the findings are relevant not only for clinical veterinary medicine but also for public health.

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Section: Discussionsupporting

confidence: 70%

Nationwide Survey of Leptospira Antibodies in Dogs in Japan: Results from Microscopic Agglutination Test and Enzyme-Linked Immunosorbent Assay

Iwamoto

Wada

Fujisaki

et al. 2009

The Journal of Veterinary Medical Science

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“…LAT was standardized with rLipL41 fusion protein as per the method of Dey et al, [13] with minor modiÞ cations.…”

Section: Latex Agglutination Testmentioning

confidence: 99%

Rapid serodiagnosis of leptospirosis by latex agglutination test and flow-through assay

Senthilkumar¹,

Subathra²,

Phil³

et al. 2008

Indian J Med Microbiol

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Purpose: Diagnosis of leptospirosis facilitates patient management and initiation of therapy. The microscopic agglutination test (MAT) is the serological test used in reference laboratories because of its high degree of sensitivity and speciÞ city. But the results are not available quickly for patient management. In the present study, in order to develop a simple, rapid immunodiagnostic assay, one of the outer membrane proteins (OMPs), recombinant LipL41 (rLipL41) has been utilised in latex agglutination test (LAT) and ß ow-through assay. Methods: Part of LipL41 gene was expressed in Escherichia coli system and puriÞ ed. The rLipL41 antigen of pathogenic Leptospira interrogans serovar Icterohaemorrhagiae, which is conserved in all pathogenic Leptospira spp. was used as capture antigen in the LAT and ß ow-through test. Both tests are very rapid and could be completed within 5 minutes. The sensitivity and speciÞ city of rLipL41 was assessed and evaluated in LAT and ß ow-through assay in comparison with standard MAT. Results: The sensitivity and speciÞ city of the LAT were 89.70 and 90.45% and ß ow-through assay were 89.09 and 77.70%, respectively. Conclusions: The developed LAT and ß ow-through assays were simple, rapid and economical for the detection of leptospira infection and suitable for large-scale screening of samples in endemic areas without any sophisticated equipment.

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“…Expressed at high levels both during cultivation and during natural infection, LipL32 was shown to be surface exposed and highly immunogenic (14,15,21,22). It has been evaluated as an antigen for immunodiagnosis (4,16,19) and as a vaccine antigen, showing protection against Leptospira interrogans challenge in animals immunized with recombinant adenovirus (6), DNA vaccine (7), or recombinant Mycobacterium bovis BCG (36).…”

mentioning

confidence: 99%

In LipL32, the Major Leptospiral Lipoprotein, the C Terminus Is the Primary Immunogenic Domain and Mediates Interaction with Collagen IV and Plasma Fibronectin

et al. 2008

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Recombinant LipL32 antigen-based single serum dilution ELISA for detection of canine leptospirosis

Cited by 71 publications

References 15 publications

Nationwide Survey of Leptospira Antibodies in Dogs in Japan: Results from Microscopic Agglutination Test and Enzyme-Linked Immunosorbent Assay

Nationwide Survey of Leptospira Antibodies in Dogs in Japan: Results from Microscopic Agglutination Test and Enzyme-Linked Immunosorbent Assay

Rapid serodiagnosis of leptospirosis by latex agglutination test and flow-through assay

In LipL32, the Major Leptospiral Lipoprotein, the C Terminus Is the Primary Immunogenic Domain and Mediates Interaction with Collagen IV and Plasma Fibronectin

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