Recombinant plasmid constructs expressing gene for antimicrobial peptide melittin for the therapy of mycoplasma and chlamydia infections

Лазарев, В. Н.; Shkarupeta, Marina M.; Kostryukova, Elena S.; Левицкий, С. А.; Titova, G. A.; Akopian, T. A.; Govorun, Vadim M.

doi:10.1007/s10517-007-0350-1

Cited by 9 publications

(4 citation statements)

References 12 publications

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“…In vitro studies have demonstrated its antibacterial activity against a wide range of microorganisms [ 19 , 73 , 89 , 90 ]. In vivo studies also provide evidence for the antibacterial activity of melittin against Chlamydia trachomatis [ 91 ], Cutibacterium acnes [ 92 ], MRSA [ 49 ] and extensively drug-resistant (XDR) A. baumannii [ 93 ]. Antibacterial properties of BV and melittin seem promising, especially for multidrug-resistant (MDR) bacteria [ 48 ].…”

Section: Antimicrobial Properties Of Bee Productsmentioning

confidence: 99%

Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products

et al. 2021

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Bee products have been known for centuries for their versatile healing properties. In recent decades they have become the subject of documented scientific research. This review aims to present and compare the impact of bee products and their components as antimicrobial agents. Honey, propolis, royal jelly and bee venom are bee products that have antibacterial properties. Sensitivity of bacteria to these products varies considerably between products and varieties of the same product depending on their origin. According to the type of bee product, different degrees of activity were observed against Gram-positive and Gram-negative bacteria, yeasts, molds and dermatophytes, as well as biofilm-forming microorganisms. Pseudomonas aeruginosa turned out to be the most resistant to bee products. An analysis of average minimum inhibitory concentration values for bee products showed that bee venom has the strongest bacterial effectiveness, while royal jelly showed the weakest antibacterial activity. The most challenging problems associated with using bee products for medical purposes are dosage and safety. The complexity and variability in composition of these products raise the need for their standardization before safe and predictable clinical uses can be achieved.

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Section: Antimicrobial Properties Of Bee Productsmentioning

confidence: 99%

Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products

et al. 2021

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“…The AMPs are among the compounds under investigation for their general antimicrobial potency, and several of them are undergoing clinical evaluation (29,30). Regarding the Mollicutes, a recombinant plasmid expressing a gene for an AMP, melittin, was introduced into cell line and animal models, and the results suggested efficacy in the treatment of infections caused by Mycoplasma hominis and Mycoplasma gallisepticum (31)(32)(33)(34). Our study may provide another promising new strategy for treating ureaplasma infections.…”

Section: Discussionmentioning

confidence: 86%

Suppression of Antimicrobial Peptide Expression by Ureaplasma Species

et al. 2014

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Ureaplasma species commonly colonize the adult urogenital tract and are implicated in invasive diseases of adults and neonates. Factors that permit the organisms to cause chronic colonization or infection are poorly understood. We sought to investigate whether host innate immune responses, specifically, antimicrobial peptides (AMPs), are involved in determining the outcome of Ureaplasma infections. THP-1 cells, a human monocytoid tumor line, were cocultured with Ureaplasma parvum and U. urealyticum. Gene expression levels of a variety of host defense genes were quantified by real-time PCR. In vitro antimicrobial activities of synthetic AMPs against Ureaplasma spp. were determined using a flow cytometry-based assay. Chromosomal histone modifications in host defense gene promoters were tested by chromatin immunoprecipitation (ChIP). DNA methylation status in the AMP promoter regions was also investigated. After stimulation with U. parvum and U. urealyticum, the expression of cell defense genes, including the AMP genes (DEFB1, DEFA5, DEFA6, and CAMP), was significantly downregulated compared to that of TNFA and IL-8, which were upregulated. In vitro flow cytometry-based antimicrobial assay revealed that synthetic peptides LL-37, hBD-3, and hBD-1 had activity against Ureaplasma spp. Downregulation of the AMP genes was associated with chromatin modification alterations, including the significantly decreased histone H3K9 acetylation with U. parvum infection. No DNA methylation status changes were detected upon Ureaplasma infection. In conclusion, AMPs have in vitro activity against Ureaplasma spp., and suppression of AMP expression might be important for the organisms to avoid this aspect of the host innate immune response and to establish chronic infection and colonization.

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“…Some peptides consist of short amino acid chains that are common antibacterial protein compounds (Yasin et al, 2004). Lazarev et al explored the use of the antimicrobial peptide melittin in treating chlamydia infections, the main active ingredient in bee venom, in the mid-1990s (Lazarev et al, 2002(Lazarev et al, , 2005(Lazarev et al, , 2007. C. trachomatis was inhibited in vitro by introducing and activating a recombinant plasmid vector expressing the melittin gene.…”

Section: Peptidesmentioning

confidence: 99%

Alternative strategies for Chlamydia treatment: Promising non-antibiotic approaches

et al. 2022

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Chlamydia is an obligate intracellular bacterium where most species are pathogenic and infectious, causing various infectious diseases and complications in humans and animals. Antibiotics are often recommended for the clinical treatment of chlamydial infections. However, extensive research has shown that antibiotics may not be sufficient to eliminate or inhibit infection entirely and have some potential risks, including antibiotic resistance. The impact of chlamydial infection and antibiotic misuse should not be underestimated in public health. This study explores the possibility of new therapeutic techniques, including a review of recent studies on preventing and suppressing chlamydial infection by non-antibiotic compounds.

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Recombinant plasmid constructs expressing gene for antimicrobial peptide melittin for the therapy of mycoplasma and chlamydia infections

Cited by 9 publications

References 12 publications

Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products

Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products

Suppression of Antimicrobial Peptide Expression by Ureaplasma Species

Alternative strategies for Chlamydia treatment: Promising non-antibiotic approaches

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