Recombinant Protein p30 for Serological Diagnosis of African Swine Fever by Immunoblotting Assay

Kazakova, Anna S.; Imatdinov, Ilnaz; Dubrovskaya, O.A.; Imatdinov, A.R.; Sidlik, M. V.; Балышев, В. М.; Krasochko, P.A.; Sereda, A.D.

doi:10.1111/tbed.12539

Cited by 27 publications

(18 citation statements)

References 36 publications

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“…ASFV p30 is a phosphorylated protein expressed at the early stage in infected cells [18]. ELISA kits based on p30 are widely used to detect ASFV infection [18][19][20]. In this study, the ELISA kit based…”

Section: Discussionmentioning

confidence: 99%

Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV

Zhao

Jiang

et al. 2021

Life

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African swine fever virus (ASFV) is an important viral pathogen infecting pigs worldwide throughout the pig industry. CD2v (an outer-membrane glycosylated protein of ASFV)-unexpressed lower-virulence mutants have appeared in China and other countries in recent years. Using OIE-recommended quantitative PCR and ELISA methods, people can accurately judge whether pigs are infected with wild-type ASFV. However, the strategy has failed to distinguish ΔCD2v lower-virulence mutants and wild-type ASFV infection. Here, we expressed and purified the CD2v and p30 proteins via CHO cells and successfully established a dual enzyme-linked immunosorbent assay (ELISA), which can be used to differentiate pigs infected with wild-type ASFV or with CD2v-unexpressed lower-virulence mutants. The dual ELISA showed excellent specificity without cross-reactions with antibodies of PRRSV, CSFV, JEV, PRV, or PPV. The dual ELISA could detect ASFV-infected positive serum samples up to dilutions of 5120 times, possessing high sensitivity. Therefore, the application of this dual ELISA approach can play an important role in ASFV epidemiology study and fill the gaps in differential diagnosis.

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Section: Discussionmentioning

confidence: 99%

Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV

Zhao

Jiang

et al. 2021

Life

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“…Kazakova et al linked protein P30 with 6xHis tag and purified to high purity recombinant protein P30 by chromatography and established an immunoblotting test system for serological diagnosis of ASFV. The specificity and sensitivity were improved [143].…”

Section: Immunoblotting Test (Ibt)mentioning

confidence: 97%

Development of Diagnostic Tests Provides Technical Support for the Control of African Swine Fever

Qiu

Yan

et al. 2021

Vaccines

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African swine fever is a highly contagious global disease caused by the African swine fever virus. Since African swine fever (ASF) was introduced to Georgia in 2007, it has spread to many Eurasian countries at an extremely fast speed. It has recently spread to China and other major pig-producing countries in southeast Asia, threatening global pork production and food security. As there is no available vaccine at present, prevention and control must be carried out based on early detection and strict biosecurity measures. Early detection should be based on the rapid identification of the disease on the spot, followed by laboratory diagnosis, which is essential for disease control. In this review, we introduced the prevalence, transmission routes, eradication control strategies, and diagnostic methods of ASF. We reviewed the various methods of diagnosing ASF, focusing on their technical characteristics and clinical test results. Finally, we give some prospects for improving the diagnosis strategy in the future.

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“…IBs using recombinant p54, a highly immunogenic ASFV protein expressed in Escherichia coli or baculovirus systems, have been described, and are easier to interpret than ASFVinfected cell-based IBs, and avoid the need for ASFV antigens produced in cells (182,191). E. coli-expressed p30 has also been demonstrated to be a highly sensitive and specific antigen for IBs, capable of detecting serological responses as early as 6-8 days post-infection (197). IBs/WBs based on individual ASFV proteins do not offer the multiple ASFV antigen array present in ASFV-infected cell lysate.…”

Section: Detection Of Asfv Antibodiesmentioning

confidence: 99%

African Swine Fever Virus: An Emerging DNA Arbovirus

et al. 2020

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African swine fever virus (ASFV) is the sole member of the family Asfarviridae, and the only known DNA arbovirus. Since its identification in Kenya in 1921, ASFV has remained endemic in Africa, maintained in a sylvatic cycle between Ornithodoros soft ticks and warthogs (Phacochoerus africanus) which do not develop clinical disease with ASFV infection. However, ASFV causes a devastating and economically significant disease of domestic (Sus scrofa domesticus) and feral (Sus scrofa ferus) swine. There is no ASFV vaccine available, and current control measures consist of strict animal quarantine and culling procedures. The virus is highly stable and easily spreads by infected swine, contaminated pork products and fomites, or via transmission by the Ornithodoros vector. Competent Ornithodoros argasid soft tick vectors are known to exist not only in Africa, but also in parts of Europe and the Americas. Once ASFV is established in the argasid soft tick vector, eradication can be difficult due to the long lifespan of Ornithodoros ticks and their proclivity to inhabit the burrows of warthogs or pens and shelters of domestic pigs. Establishment of endemic ASFV infections in wild boar populations further complicates the control of ASF. Between the late 1950s and early 1980s, ASFV emerged in Europe, Russia and South America, but was mostly eradicated by the mid-1990s. In 2007, a highly virulent genotype II ASFV strain emerged in the Caucasus region and subsequently spread into the Russian Federation and Europe, where it has continued to circulate and spread. Most recently, ASFV emerged in China and has now spread to several neighboring countries in Southeast Asia. The high morbidity and mortality associated with ASFV, the lack of an efficacious vaccine, and the complex makeup of the ASFV virion and genome as well as its lifecycle, make this pathogen a serious threat to the global swine industry and national economies. Topics covered by this review include factors important for ASFV infection, replication, maintenance, and transmission, with attention to the role of the argasid tick vector and the sylvatic transmission cycle, current and future control strategies for ASF, and knowledge gaps regarding the virus itself, its vector and host species.

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Recombinant Protein p30 for Serological Diagnosis of African Swine Fever by Immunoblotting Assay

Cited by 27 publications

References 36 publications

Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV

Development of a Dual ELISA for the Detection of CD2v-Unexpressed Lower-Virulence Mutational ASFV

Development of Diagnostic Tests Provides Technical Support for the Control of African Swine Fever

African Swine Fever Virus: An Emerging DNA Arbovirus

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