Recommendations for good practice in MS-based lipidomics

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“…Readers interested in this area of work could also find further discussion by Kofeler et al. ( 188 ) in this Thematic Review series.…”

Section: Current Status Of Lipidomicsmentioning

confidence: 96%

The foundations and development of lipidomics

Han

Gross

2022

Journal of Lipid Research

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115

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“…RP-LC-MS allows separation of lipid molecules according to their fatty acyl chains, while separation in NP-or HILIC-LC-MS occurs according to their polar head groups, separating lipid class. Different mass spectrometers have been used in lipidomic approaches, but more recently high-resolution MS using Orbitrap, or quadrupole time-offlight (Q-TOF) instruments are more frequently used because they allow accurate mass information to be obtained from molecular ions, as well as fragment ions, in addition to MS/MS interpretation [59]. DI-MS is usually used with target lipidomics based on shotgun approaches [60].…”

Section: Lipidomics Approachmentioning

confidence: 99%

Adaptation of Lipid Profiling in Depression Disease and Treatment: A Critical Review

Pinto

Conde

Domingues

et al. 2022

IJMS

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Major depressive disorder (MDD), also called depression, is a serious disease that impairs the quality of life of patients and has a high incidence, affecting approximately 3.8% of the world population. Its diagnosis is very subjective and is not supported by measurable biomarkers mainly due to the lack of biochemical markers. Recently, disturbance of lipid profiling has been recognized in MDD, in animal models of MDD or in depressed patients, which may contribute to unravel the etiology of the disease and find putative new biomarkers, for a diagnosis or for monitoring the disease and therapeutics outcomes. In this review, we provide an overview of current knowledge of lipidomics analysis, both in animal models of MDD (at the brain and plasma level) and in humans (in plasma and serum). Furthermore, studies of lipidomics analyses after antidepressant treatment in rodents (in brain, plasma, and serum), in primates (in the brain) and in humans (in plasma) were reviewed and give evidence that antidepressants seem to counteract the modification seen in lipids in MDD, giving some evidence that certain altered lipid profiles could be useful MDD biomarkers for future precision medicine.

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“…Mass spectrometry of glycerophospholipids enables identification based on their mass-to-charge value (m/z), and further structural elucidation can be provided by the use of tandem mass spectrometry, MS 2 , and MS n [11]. In particular, the head group of glycerophospholipids is readily determined in MS 2 by identifying and/or scanning for the specific head group product ions or neutral losses (NL) [11,12]. The acyl chain composition and position on the glycerol backbone are more challenging to determine by tandem mass spectrometry.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of sn-positional phospholipid isomers in MSn using silver cationization

Lillja

Lanekoff

2022

Anal Bioanal Chem

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Glycerophospholipids are one of the fundamental building blocks for life. The acyl chain connectivity to the glycerol backbone constitutes different sn-positional isomers, which have great diversity and importance for biological function. However, to fully realize their impact on function, analytical techniques that can identify and quantify sn-positional isomers in chemically complex biological samples are needed. Here, we utilize silver ion cationization in combination with tandem mass spectrometry (MSn) to identify sn-positional isomers of phosphatidylcholine (PC) species. In particular, a labile carbocation is generated through a neutral loss (NL) of AgH, the dissociation of which provides diagnostic product ions that correspond to acyl chains at the sn-1 or sn-2 position. The method is comparable to currently available methods, has a sensitivity in the nM–µM range, and is compatible with quantitative imaging using mass spectrometry in MS4. The results reveal a large difference in isomer concentrations and the ion images show that the sn-positional isomers PC 18:1_18:0 are homogeneously distributed, whereas PC 18:1_16:0 and PC 20:1_16:0 show distinct localizations to sub-hippocampal structures. Graphical abstract

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Recommendations for good practice in MS-based lipidomics

Cited by 120 publications

References 130 publications

The foundations and development of lipidomics

The foundations and development of lipidomics

Adaptation of Lipid Profiling in Depression Disease and Treatment: A Critical Review

Quantitative determination of sn-positional phospholipid isomers in MSn using silver cationization

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