Recommendations for the performance of bacterial mutation assays

Gatehouse, D.; Haworth, Steve; Cebula, Thomas A.; Gocke, Elmar; Kier, Larry D.; Matsushima, Taijiro; Melción, C.; Nohmi, Takehiko; Ohta, Toshihiro; Venitt, S.; Zeiger, Errol

doi:10.1016/0165-1161(94)90037-x

Cited by 195 publications

(75 citation statements)

References 30 publications

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“…The positive controls in the absence of metabolic activator (-S9) were 2-aminofluorine (2-AF) for TA98 and TA100. Each determination was made in triplicate and at least two independent experiments were carried out to confirm the results (Gatehouse et al, 1994).…”

Section: Mutagenicity Assaymentioning

confidence: 99%

Temporal and Spatial Distribution of Particulate Carcinogens and Mutagens in Bangkok, Thailand

Pongpiachan

Choochuay²,

Hattayanone³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Section: Mutagenicity Assaymentioning

confidence: 99%

Temporal and Spatial Distribution of Particulate Carcinogens and Mutagens in Bangkok, Thailand

Pongpiachan

Choochuay²,

Hattayanone³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…According to latest Guidance on classification of chemicals [27], the classification of individual substanc- activity [31,32]; Gatehouse, et al, 1994) [31][32][33]. It is widely accepted that positive results in the Ames test correlate well with carcinogenic potential, at least in rodents (70%-90% predictivity; [28].…”

Section: Testing For Mutagenicity Potential According To Regulatory Cmentioning

confidence: 99%

Testing the Mutagenicity Potential of Chemicals

Verheyen¹

2017

J Genet Genome Res

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All information for the proper development, functioning and reproduction of organisms is coded in the sequence of matched base-pairs of DNA. DNA mutations can result in harmful effects and play a role in genetic disorders and cancer. As mutations can arise through exposure to chemical substances, testing needs to be done on substances that humans and animals can be exposed to. This review focuses on the different testing strategies for risk assessment of chemicals for genotoxicity and carcinogenicity. This review is not meant to cover all testing methodologies, but rather to give an overview of the main methodologies that are used in a regulatory context. In silico and in vitro tools are playing an increasingly important role, in order to reduce in vivo animal testing. Especially in silico tools like (Q)SARs are promising as they are non-testing methods and thereby also reduce the costs and time that are needed to perform in vitro assays. (Q)SARs are not sufficiently reliable to be used as stand-alone tools, and should be associated with expert judgment or used in combination with other Weight of Evidence such as in vitro and in vivo data and data derived from other similar molecules (read-across). For some product (sub) categories, testing (in vitro/in vivo) will be unavoidable; however the future may bring reliable approaches for ensuring human safety.

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“…The mutagenic activity of these compounds were assessed by the Salmonella microsome assay (Ames test), using S. typhimurium test strains TA98, TA97a (to detect frameshift mutations), TA100 (to detect base-pair-substitution mutations) and TA102 (normally used to detect mutagens that cause oxidative damage and base-pair-substitution mutations), in the presence or absence of in vitro metabolizing systems [14]- [16]. Results of genetic toxicological tests, combined with an adequate pharmacology profile, have been used to approve clinical trials of novel drug candidates [17].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Safety of Three Phenolic Compounds from <i>Dipteryx alata</i> Vogel with Antiophidian Potential

Yoshida¹,

Ferraz²,

Tribuiani³

et al. 2015

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Phenolic compounds from Dipteryx alata Vogel were assayed against the in vitro neurotoxic effect induced by Bothrops jararacussu (Bjssu) venom. Mutagenicity was assessed by the Ames test using Salmonella typhimurium strains TA98, TA97a, TA100, and TA102, in experiments with and without metabolic activation. Anti-bothropic activity was obtained by using mouse phrenic nerve-diaphragm (PND) preparation and myographic technique. Control experiments with physiological Tyrode solution were used for keeping the PND preparations alive (n = 4). Concentrations of phenolic compounds were as follow: protocatechuic and vanillic acids (200 µg/mL, n = 4), vanillin (50 µg/mL, n = 4). These compounds were used alone or pre-incubated with the venom (40 µg/mL), 30 min prior the addition to the organ bath (n = 4). Phenolic compounds significantly inhibited the neuromuscular blockade of Bjssu in the following order of potency: vanillic acid > protocatechuic = vanillin. Vanillic acid added 10 min after the Bjssu venom was also able to avoid the venomblockade evolution. The mutagenicity assay indicated that all phytochemicals were unable to increase the number of revertants, demonstrating the absence of mutagenic activity. This study * Corresponding author. E. H. Yoshida et al.2 demonstrated both the safety and therapeutical potential of the three phenolic compounds as novel complementary anti-bothropic agents.

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Recommendations for the performance of bacterial mutation assays

Cited by 195 publications

References 30 publications

Temporal and Spatial Distribution of Particulate Carcinogens and Mutagens in Bangkok, Thailand

Temporal and Spatial Distribution of Particulate Carcinogens and Mutagens in Bangkok, Thailand

Testing the Mutagenicity Potential of Chemicals

Evaluation of the Safety of Three Phenolic Compounds from <i>Dipteryx alata</i> Vogel with Antiophidian Potential

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Recommendations for the performance of bacterial mutation assays

Cited by 195 publications

References 30 publications

Temporal and Spatial Distribution of Particulate Carcinogens and Mutagens in Bangkok, Thailand

Temporal and Spatial Distribution of Particulate Carcinogens and Mutagens in Bangkok, Thailand

Testing the Mutagenicity Potential of Chemicals

Evaluation of the Safety of Three Phenolic Compounds from &lt;i&gt;Dipteryx alata&lt;/i&gt; Vogel with Antiophidian Potential

Contact Info

Product

Resources

About

Evaluation of the Safety of Three Phenolic Compounds from <i>Dipteryx alata</i> Vogel with Antiophidian Potential