Reconfigurable Implication and Inhibition Boolean logic gates based on NAD⁺‐dependent enzymes: Application to signal‐controlled biofuel cells and molecule release

Abstract

The Implication and Inhibition Boolean logic gates were realized using NAD+/NADH‐dependent dehydrogenases combined with hexokinase competing for biomolecule input signals. Both logic gates operated with the same enzyme composition and their reconfiguration was achieved simply by redefining the input signals. The output signals produced by the logic gates were analyzed optically and electrochemically, particularly using enzyme‐modified electrodes. The logically processed input signals were used to switch operation of a biofuel cell and activate a molecule release process.