Reconfigurable Lab-on-Chip platform for algae cell manipulation

Miled, Amine

doi:10.1109/iscas.2014.6865218

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…Song et al 213 also reported a constriction-based iDEP device for separation of C. vulgaris and P. subcapitata. Miled et al 214 reported a microelectrode array device for pDEP-and nDEPbased manipulation of algae cells. Graham et al 215 used a commercially available metal microelectrode for dielectrophoretic manipulation of E. viridis cells and intercellular organelles.…”

Section: ■ Technical Realization Platforms For Depmentioning

confidence: 99%

Dielectrophoresis: From Molecular to Micrometer-Scale Analytes

2018

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Section: ■ Technical Realization Platforms For Depmentioning

confidence: 99%

Dielectrophoresis: From Molecular to Micrometer-Scale Analytes

2018

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“…Furthermore, some LoC are fully integrated systems with electrodes covered by a membrane inside microfluidic micro-channels while other systems use electrodes fabricated on a simple printed circuit board (PCB) [9]- [11]. Obviously, it is practically impossible to have a test-bench setup versatile enough to cover all kinds of applications where LoC are used.…”

mentioning

confidence: 99%

“…In addition the same platform was used to detect algae concentration in water. Obtained results are published in [11].…”

mentioning

confidence: 99%

Reconfigurable Prototyping Microfluidic Platform for DEP Manipulation and Capacitive Sensing

Miled

Auclair²,

Srasra³

2015

IEEE Trans. Biomed. Circuits Syst.

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In this paper, we present a new rapid prototyping platform dedicated to dielectrophoretic microfluidic manipulation and capacitive cell sensing. The proposed platform offers a reconfigurable design including 4 independently programmable output channels to be distributed across 64 electrodes. Although its range of frequency covers up to 3.4 MHz, signal amplitude accuracy ( +/-10%) was demonstrated for frequencies up to 1 MHz and channel-to-channel phase shift setting was stable up to 1.5 MHz. A test of maximum resistive load showed a 10% attenuation of a 12 V peak-to-peak signal with a 22 Ω load. The platform has an advanced capacitive sensor to measure capacitance variation between in-channel electrodes with a sampling frequency up to 5 kH z. Experimental data of capacitive sensor showed a sensitivity of 100 fF. The sensor can be extended to 4 parallel measurements with lower frequency. We also present a new assembly technique for reusable microfluidic chip based on anisotropic adhesive conductive film, epoxy and PDMS. The proposed platform provides a wide range of control signals depending on the type of manipulation as sine, rectangular or square wave. The frequency range is extendible up to 3.4 MHz, in addition to a programmable phase shift circuit with a minimum phase step of 3.6(°) for each signal.

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Separation, Characterization, and Handling of Microalgae by Dielectrophoresis

et al. 2020

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Microalgae biotechnology has a high potential for sustainable bioproduction of diverse high-value biomolecules. Some of the main bottlenecks in cell-based bioproduction, and more specifically in microalgae-based bioproduction, are due to insufficient methods for rapid and efficient cell characterization, which contributes to having only a few industrially established microalgal species in commercial use. Dielectrophoresis-based microfluidic devices have been long established as promising tools for label-free handling, characterization, and separation of broad ranges of cells. The technique is based on differences in dielectric properties and sizes, which results in different degrees of cell movement under an applied inhomogeneous electrical field. The method has also earned interest for separating microalgae based on their intrinsic properties, since their dielectric properties may significantly change during bioproduction, in particular for lipid-producing species. Here, we provide a comprehensive review of dielectrophoresis-based microfluidic devices that are used for handling, characterization, and separation of microalgae. Additionally, we provide a perspective on related areas of research in cell-based bioproduction that can benefit from dielectrophoresis-based microdevices. This work provides key information that will be useful for microalgae researchers to decide whether dielectrophoresis and which method is most suitable for their particular application.

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Reconfigurable Lab-on-Chip platform for algae cell manipulation

Cited by 3 publications

References 6 publications

Dielectrophoresis: From Molecular to Micrometer-Scale Analytes

Dielectrophoresis: From Molecular to Micrometer-Scale Analytes

Reconfigurable Prototyping Microfluidic Platform for DEP Manipulation and Capacitive Sensing

Separation, Characterization, and Handling of Microalgae by Dielectrophoresis

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