Reconstitute the damaged heart via the dual reparative roles of pericardial adipose-derived flk-1+ stem cells

Wang, Xiaoming; Liu, Xueqing; Zhang, Hui; Nie, Liangming; Chen, Min; Ding, Zhaoping

doi:10.1016/j.ijcard.2015.09.002

Cited by 9 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…WT1 pos cells were eventually flk-1 negative, indicating that they were phenotypically distinct subset from pADSC that we have previously reported (Fig. 2c ) [ 13 ]. Given the cardiac potential of WT1 pos cells, we further compared a panel of important transcriptional factors that regulate myogenic differentiation.…”

Section: Resultssupporting

confidence: 66%

“…4 ). The beneficial effects observed in the present experiment were more pronounced than previously reported results either using the unfractionated population [ 12 ] or the flk-1-positive pADSC tested in our laboratory [ 13 ]. Since WT1 pos cells likewise failed to adapt to long-term engraftment in the host myocardium (data not shown), the reparative activity can be most probably attributed to a paracrine effect rather than direct formation of de novo cardiomyocytes [ 12 ].…”

Section: Discussioncontrasting

confidence: 60%

“…In our previous experiments characterizing the reparative activity of pADSC the pericardial tissue samples were also sometimes taken from MI rats [ 12 , 13 ] and we found, unexpectedly, that the pADSC isolated from the MI rats exhibited significantly enhanced reparative properties in comparison with the cells from healthy animals. We therefore compared the phenotypic markers of pADSC from two types of animals, in other words healthy and MI rats.…”

Section: Discussionmentioning

confidence: 70%

“…However, it remains elusive whether the pericardial progenitors residing in the pericardial tissue could recapitulate an embryonic program in response to cardiac injury [ 8 ]. This question is of practical interest since pericardial adipose tissue is abundant and clinically easy to access, and thus the re-activated pericardial adipose-derived stem cells (pADSC) may represent one of the most suitable cell donors for cell-based therapy for cardiac ischemic disease [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Injury-induced fetal reprogramming imparts multipotency and reparative properties to pericardial adipose stem cells

Tang

Wang²,

Tan

et al. 2018

Stem Cell Res Ther

Self Cite

View full text Add to dashboard Cite

BackgroundInjury may induce a sequential activation of intrinsic reparative activity that supports the maintenance of tissue homeostasis.MethodIn the present experiments, we investigated whether myocardial infarction (MI) was able to reinstate the expression of Wilms’ tumor factor 1 (WT1) as a key hallmark of fetal reprograming in the pericardial adipose-derived stem cells (pADSC). We characterized the immunophenotypical markers, cardiac potential, and reparative activity of WT1-expressing pADSC (WT1pos) isolated MI Wistar rats with an intact pericardial sac in which cardiac transudate was accumulated, sampled, and analyzed.ResultsThe WT1pos cells formed colony-like aggregates in culture that subsequently generated phase-bright cells that homogenously constituted WT1 expression (> 98%). The WT1pos cells shared identical surface markers with canonical pADSC, but enhanced transcripts for cardiogenesis (isl-1, gata-4, Sox2 and Tbx18) as well as cardiac commitment (endothelial: 28%; cardiomyogenic: 12.3%) in defined conditions. Remarkably, cardiac transplantation of WT1pos cells promoted regional angiogenesis and myogenesis which led to significant functional amelioration of the infarcted hearts. Furthermore, we demonstrated that WT1pos cells uniquely secreted hepatocyte growth factor (HGF) as a key antiapoptotic factor that promotes cardiac repair.ConclusionInjury-associated fetal reprogramming in pADSC facilitates cardiac differentiation and promotes the reparative activity by enhancing HGF production. As such, injury-“conditioned” pADSC may represent a useful autologous cell donor from infarcted patients for cell-based therapy.

show abstract

Section: Resultssupporting

confidence: 66%

Section: Discussioncontrasting

confidence: 60%

Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Injury-induced fetal reprogramming imparts multipotency and reparative properties to pericardial adipose stem cells

Tang

Wang²,

Tan

et al. 2018

Stem Cell Res Ther

Self Cite

View full text Add to dashboard Cite

show abstract

“…A murine model of myocardial infarction (MI, 60 min ischemia+reperfusion) was induced as previously described [16]. In brief, mice were intubated and anesthetized by mechanical ventilation with isoflurane (1.5% v / v ) in 80% oxygen/20% nitrogen.…”

Section: Methodsmentioning

confidence: 99%

CD73 Expression on Mesenchymal Stem Cells Dictates the Reparative Properties via Its Anti-Inflammatory Activity

Tan

Zhu²,

Zhang³

et al. 2019

Stem Cells International

Self Cite

View full text Add to dashboard Cite

Mesenchymal stem cells (MSC) are not universal and may be subject to dynamic changes upon local milieus in vivo and after isolation and cultivation in vitro. Here, we demonstrate that MSC derived from murine pericardial adipose tissue (pMSC) constitute two cohorts of population distinguished by the level of CD73 expression (termed as CD73high and CD73low pMSC). Transplantation of two types of cells into mouse hearts after myocardial infarction (MI) revealed that the CD73high pMSC preferentially brought about structural and functional repair in comparison to the PBS control and CD73low pMSC. Furthermore, the CD73high pMSC displayed a pronounced anti-inflammatory activity by attenuating CCR2+ macrophage infiltration and upregulating several anti-inflammatory genes 5 days after in vivo transplantation and ex vivo cocultivation with peritoneal macrophages. The immunomodulatory effect was not seen in cocultivation experiments with pMSC derived from CD73 knockout mice (CD73-/-) but was partially blocked by pretreatment of the A2b receptor antagonist, PSB603. The results highlight a heterogeneity of the CD73 expression that may be related to its catalytic products on the modulation of the local immune response and thus provide a possible explanation to the inconsistency of the regenerative results when different sources of donor cells were used in stem cell-based therapy.

show abstract

Myocardial regeneration: role of epicardium and implicated genes

et al. 2019

View full text Add to dashboard Cite

Reconstitute the damaged heart via the dual reparative roles of pericardial adipose-derived flk-1+ stem cells

Cited by 9 publications

References 30 publications

Injury-induced fetal reprogramming imparts multipotency and reparative properties to pericardial adipose stem cells

Injury-induced fetal reprogramming imparts multipotency and reparative properties to pericardial adipose stem cells

CD73 Expression on Mesenchymal Stem Cells Dictates the Reparative Properties via Its Anti-Inflammatory Activity

Myocardial regeneration: role of epicardium and implicated genes

Contact Info

Product

Resources

About