Recovery Efficiency of Cumulus Oocyte Complexes (COCs) according to Collection Frequency for Ovum Pick-up (OPU) Method in Hanwoo Cow

Kang, Sung-Sik; Kim, Ui-Hyung; Lee, Seok-Dong; Lee, Myung-Suk; Han, Manhye; Cho, Sang-Rae

doi:10.12750/jarb.34.4.300

Cited by 8 publications

(3 citation statements)

References 13 publications

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“…In Bos indicus, an average of 18-25 COCs per head were recovered using OPU [9,18]. On average, B. indicus has more follicular waves and follicles larger than 5 mm than B. taurus.…”

Section: Discussionmentioning

confidence: 99%

Sex ratio and conception rates of fresh/vitrified embryos at different developmental stages by ovum pick up in Hanwoo cows

Park,

Bang,

Lee

et al. 2024

J Anim Sci Technol

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Embryo transfer plays a crucial role in enhancing the breeding value of livestock; it has been applied in Hanwoo cattle, which is a popular breed for beef production in Korea. Both in vivo-derived (IVD) and in vitro-produced (IVP) embryos are used for this purpose; however, IVP embryos have been preferred recently owing to advancements in ovum pick-up (OPU) technology and genomic selection. Despite technological advancements, comprehensive data on large-scale OPU/IVEP/embryo transfer in Hanwoo cows are lacking. In this study, 16 elite Hanwoo donor cows were selected on the basis of specific criteria.Oocytes were retrieved from 241 cows using OPU. The collected cumulus-oocyte complexes (COCs) were matured, fertilized, and cultured in vitro to produce transferable embryos. Embryos were classified according to their developmental stage and then transferred to 675 recipient cows. A total of 3,317 COCs were collected, with an average of 13.76 COCs per cow. The number of transferable embryos produced per cow was 3.7. Hanwoo OPU-derived IVP embryos exhibited a higher production yield than the global average, indicating a stable IVEP environment. Both fresh and frozen IVP embryos yielded similar conception rates; hence, the use of vitrified-thawed embryos in transfer plans feasible. However, frozenthawed embryos at Stage 7 had a lower conception rate than those at earlier stages. There was no significant difference between the conception rates of sexually mature heifers and postpartum cows used as recipients.The male-to-female offspring ratio increased as the developmental stage progressed. Seasonal effects on conception rates were not observed; however, higher abortion rates and a higher proportion of male offspring were observed during winter. This study provides valuable data for the Korean embryo transfer industry, enabling more strategic growth of the domestic Hanwoo embryo industry.

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“…In Bos indicus, an average of 18-25 COCs per head were recovered using OPU [9,18]. On average, B. indicus has more follicular waves and follicles larger than 5 mm than B. taurus.…”

Section: Discussionmentioning

confidence: 99%

Sex ratio and conception rates of fresh/vitrified embryos at different developmental stages by ovum pick up in Hanwoo cows

Park,

Bang,

Lee

et al. 2024

J Anim Sci Technol

View full text Add to dashboard Cite

show abstract

“…In Bos indicus cattle, using OPU, an average of 18–25 COCs were recovered per head [ 38 , 46 ]. On average, Bos indicus has more follicular waves and follicles larger than 5 mm compared to Bos taurus .…”

Section: Discussionmentioning

confidence: 99%

Improving Cryopreservation Efficiency and Pregnancy Rate through Superovulation with Follicle-Stimulating Hormone in Korean Hanwoo Cows via Ovum Pick Up

Kim,

2023

Veterinary Sciences

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The aim of this study was to devise an efficient technique for generating embryos from high-quality bovine females. Oocytes were collected from 20 control and 15 Hanwoo (Bos taurus coreanae) females treated with the FSH. A combination of decreasing FSH doses (36, 36, 24, and 24 mg, 12 h apart), progesterone, estrogen, and prostaglandins were administered to synchronize and mildly stimulate the animals. The FSH-treated group (1125 oocytes) and control group (1022 oocytes) exhibited a higher proportion of grade A and B oocytes (88.2%) than the other grades (p < 0.05), with most at the germinal vesicle 2 stage (64.0%). Moreover, the FSH-treated group achieved a notably higher blastocyst rate (44.7%) compared to the control group (31.1%) (p < 0.05). After undergoing vitrification and in vitro culture (IVC) warming, embryos in the FSH group exhibited higher re-expansion rates (grade 1: 86.9%; grades 2 and 3: 57.9%) compared to those in the control (p < 0.05). This highlights the positive impact of FSH treatment on in vitro embryo production (IVEP) and the OPU rate.

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“…Embryo transfer in cattle can accelerate the rate of genetic improvement compared to artificial insemination, particularly through the breeding of high-value female donors [ 1 , 2 ]. In Hanwoo cattle ( Bos taurus coreanae ), embryo transfer is performed for commercial purposes to enhance the quality and quantity of beef meat, with males typically raised for fattening and females for breeding [ 3 , 4 ]. With the accelerating development in embryo transfer technologies, understanding their impact on pregnancy rates and the male-female sex ratio remains an important area of research to improve the livestock industry.…”

Section: Introductionmentioning

confidence: 99%

Improved pregnancy rate and sex ratio in fresh/frozen in vivo derived embryo transfer of Hanwoo (Bos taurus coreanae) cows

Park¹,

Lee²,

Saadeldin³

et al. 2023

J Anim Sci Technol

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Recovery Efficiency of Cumulus Oocyte Complexes (COCs) according to Collection Frequency for Ovum Pick-up (OPU) Method in Hanwoo Cow

Cited by 8 publications

References 13 publications

Sex ratio and conception rates of fresh/vitrified embryos at different developmental stages by ovum pick up in Hanwoo cows

Sex ratio and conception rates of fresh/vitrified embryos at different developmental stages by ovum pick up in Hanwoo cows

Improving Cryopreservation Efficiency and Pregnancy Rate through Superovulation with Follicle-Stimulating Hormone in Korean Hanwoo Cows via Ovum Pick Up

Improved pregnancy rate and sex ratio in fresh/frozen in vivo derived embryo transfer of Hanwoo (Bos taurus coreanae) cows

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