Recurrent extragenital herpes simplex type 2 occurring in a distal location of the same dermatome

Eksomtramage, Thanadon; Aiempanakit, Kumpol

doi:10.1016/j.idcr.2020.e00783

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…However, brightfield microscopy can be used to image cells and tissues infected with the virus. The specimen for this method is collected by scraping the lesion (Tzanck smears) using a scalpel blade and transferring the sample to a glass slide, followed by staining with Giemsa, methylene blue, or toluidine blue [ 43 , 44 , 45 ]. These stained tissues are imaged in order to detect HSV cytopathic effects.…”

Section: Detection Of Hsv In Lesionsmentioning

confidence: 99%

“… ( A ) Microscopic image showing large multinucleated giant cells (indicated by arrows) surrounded by multiple normal cells in an HSV-infected patient. The cells were stained with methylene blue, taken using an optical brightfield microscope [ 45 ]. ( B ) Images showing Vero/hSLAM cells coinfected by MeV and HSV-1 from different passages and a cell control (3rd, 6th, 10th, CC).…”

Section: Figurementioning

confidence: 99%

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Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Nath

Kabir

Doust

et al. 2021

Infectious Disease Reports

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Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.

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Section: Detection Of Hsv In Lesionsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Nath

Kabir

Doust

et al. 2021

Infectious Disease Reports

View full text Add to dashboard Cite

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Two-dimensional microlens array for low-cost high-resolution bio-imaging

Khoubafarin

Nath²,

Popofski³

et al. 2023

Optics and Biophotonics in Low-Resource Settings IX

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Mobile microscopes, which are cost-effective and field-portable, are rapidly gaining popularity for a variety of different applications, including disease diagnostics. Different imaging modalities, based on transmission, absorption, scattering, phase change, and fluorescence have been developed, depending on the specific application. Mobile microscopes are typically designed to have single magnification, and the maximum achievable resolution is limited by the numerical aperture (N.A.) of the objective lens, due to diffraction of light. Here we present a low-cost imaging substrate containing a two-dimensional (2D) microlens array that enables multi-modal imaging (phase-contrast, dark-field, and fluorescence) with resolution beyond the diffraction limit. The substrate is placed in contact with the sample to form a sandwich structure and the image magnification is attributed to the formation of virtual images by the individual microspheres. This reusable substrate can be easily attached to and detached from the sample, with minimal to no sample damage. A variety of different sizes of glass microspheres (40- 500 𝜇m) were explored and their performance was characterized using fluorescent particles (200 nm – 1 μm). The substrate enabled imaging with sub-400nm resolution, using a low magnification objective lens with N.A.~0.25. Proof-of-concept experiments with this substrate were performed by imaging mammalian tissues, red blood cells, including sickle cells. This technique provides a low-cost, easy- to-use method of improving the resolution of multi-modal imaging systems, which is particularly useful for mobile microscopy applications.

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Recurrent extragenital herpes simplex type 2 occurring in a distal location of the same dermatome

Cited by 2 publications

References 6 publications

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Two-dimensional microlens array for low-cost high-resolution bio-imaging

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