Red blood cell deformability is diminished in patients with Chronic Fatigue Syndrome

Saha, Amit K.; Schmidt, Brendan R.; Wilhelmy, Julie; Nguyen, Vy; Abugherir, Abed; Justin, Koffi Djinguin Ben; Nemat‐Gorgani, Mohsen; Davis, Ronald W.; Ramasubramanian, Anand K.

doi:10.3233/ch-180469

Cited by 42 publications

(42 citation statements)

References 16 publications

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“…RBC deformability has been shown to be affected by ALT [34], AST [35], CRP [36], and 25-OH vitamin D levels [37]. Interestingly, recently, RBC deformability has been found to be impaired also in patients with chronic fatigue syndrome [38].…”

Section: Discussionmentioning

confidence: 99%

Decrease in Serum Vitamin D Level of Older Patients with Fatigue

et al. 2019

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Fatigue is characterized by reduced energy level, decreased muscle strength, and a variable degree of cognitive impairment. Recent evidences seem to link vitamin D deficiency to fatigue. The aim of this study was to assess and compare vitamin D status in a cohort of older subjects with and without fatigue. We recruited a total of 480 subjects, 240 patients with fatigue and 240 controls without fatigue, from the Cannizzaro Hospital of Catania (Italy). Fatigue severity was measured by the fatigue severity scale, whereas mental and physical fatigue were measured through the Wessely and Powell fatigue scale, respectively. We also measured several blood parameters and 25-OH vitamin D. Subjects with fatigue showed lower levels of vitamin D as compared with those without fatigue. Blood levels of parameters related to fatigue were normal in both groups of subjects, however, platelet, hemoglobin, hematocrit (p < 0.05), mean corpuscular volume, C-reactive protein (CRP), iron, vitamin B12, and folic acid (p < 0.001) were significantly higher in the fatigue group with respect to the control group. Moreover, compared to controls, patients showed higher scores in the physical (p < 0.001), mental (p < 0.001), and severity (p < 0.001) fatigue scales. Finally, vitamin D inversely correlated with fatigue severity (r = −0.428, p < 0.01), whereas creatine kinase and CRP levels did not correlate with vitamin D. In conclusion, our data showed a direct link between vitamin D and fatigue in older subjects, suggesting translational implications in the diagnosis and management of these patients.

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Section: Discussionmentioning

confidence: 99%

Decrease in Serum Vitamin D Level of Older Patients with Fatigue

et al. 2019

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“…6A-C). Ramasubramanian et al has reported higher levels of reactive oxygen species (ROS) in red blood cells of ME-patients compared to healthy controls (43), and up-regulation of the Oxidative stress response pathway upon INMEST (SIRT1), corroborates this observation. These upregulated pathways and the induction of genes in the insulin receptor signaling pathway, Heat shock proteins, and cell cycle regulators all support the idea that INMEST treatment upregulates disease tolerance pathways normally induced during infections, and possibly improperly suppressed in ME-patients ( Fig.…”

Section: Me -A Failure Of Inducing Disease Tolerance Upon Chronic Immmentioning

confidence: 82%

Achieving symptom relief in patients with Myalgic encephalomyelitis by targeting the neuro-immune interface and inducing disease tolerance

Lakshmikanth

et al. 2020

Preprint

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Myalgic encephalomyelitis, ME, previously also known as chronic fatigue syndrome (CFS) is a heterogeneous, debilitating syndrome of unknown etiology responsible for long-lasting disability in millions of patients worldwide. The most well-known symptom of ME is post-exertional malaise, but many patients also experience autonomic dysregulation, cranial nerve dysfunction and signs of immune system activation. Many patients also report a sudden onset of disease following an infection. The brainstem is a suspected focal point in ME pathogenesis and patients with structural impairment to the brainstem often show ME-like symptoms. The brainstem is also where the vagus nerve originates, a critical neuro-immune interface and mediator of the inflammatory reflex which regulate systemic inflammation. Here we report the results of a randomized, placebo-controlled trial using intranasal mechanical stimulation (INMEST) targeting the vagus nuclei, and higher centers in the brain of ME-patients and induce a sustainable, ~30% reduction in overall symptom scores after eight weeks of treatment. By performing longitudinal, systemslevel monitoring of the blood immune system in these patients, we uncover chronic immune activation in ME, as well as immunological correlates of improvement that center around the IL-17 axis, gut-homing immune cells and reduced inflammation. The mechanisms of symptom relief remains to be determined, but transcriptional analyses suggest an upregulation of disease tolerance mechanisms. We wish for these results to bring some hope to patients suffering from ME and inspire researchers to help test our new hypothesis that ME is a condition caused by a failure of inducing disease tolerance upon infection and persistent immune activation.

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“…Polymers such as Polytetrafluoroethylene (PTFE), Poly (methyl methacrylate) (PMMA), Polydimethylsiloxane (PDMS) are also being used as substrates to fabricate these devices. Polymers are inexpensive and microchannels are created by embossing and molding (Saha et al, 2017b , 2019 ). Due to low autofluorescence, biocompatibility, non-toxicity, curing at low temperatures, PDMS is the most commonly used polymer.…”

Section: Commonly Used Fabrication Techniques For Poct Devicesmentioning

confidence: 99%

Microfluidic Point-of-Care Testing: Commercial Landscape and Future Directions

Sachdeva

Davis

Saha

2021

Front. Bioeng. Biotechnol.

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181

108

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Point-of-care testing (POCT) allows physicians to detect and diagnose diseases at or near the patient site, faster than conventional lab-based testing. The importance of POCT is considerably amplified in the trying times of the COVID-19 pandemic. Numerous point-of-care tests and diagnostic devices are available in the market including, but not limited to, glucose monitoring, pregnancy and infertility testing, infectious disease testing, cholesterol testing and cardiac markers. Integrating microfluidics in POCT allows fluid manipulation and detection in a singular device with minimal sample requirements. This review presents an overview of two technologies - (a.) Lateral Flow Assay (LFA) and (b.) Nucleic Acid Amplification - upon which a large chunk of microfluidic POCT diagnostics is based, some of their applications, and commercially available products. Apart from this, we also delve into other microfluidic-based diagnostics that currently dominate the in-vitro diagnostic (IVD) market, current testing landscape for COVID-19 and prospects of microfluidics in next generation diagnostics.

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Red blood cell deformability is diminished in patients with Chronic Fatigue Syndrome

Cited by 42 publications

References 16 publications

Decrease in Serum Vitamin D Level of Older Patients with Fatigue

Decrease in Serum Vitamin D Level of Older Patients with Fatigue

Achieving symptom relief in patients with Myalgic encephalomyelitis by targeting the neuro-immune interface and inducing disease tolerance

Microfluidic Point-of-Care Testing: Commercial Landscape and Future Directions

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