Red orange and lemon extract preserve from oxidative stress, DNA damage and inflammatory status in lambs

Damiano, Sara; Longobardi, Consiglia; Salzano, Angela; D’Angelo, Livia; Amenta, Margherita; Maggiolino, Aristide; Palo, Pasquale De; Claps, Salvatore; Rufrano, Domenico; Iannaccone, Francesco; Matera, Roberta; Ciarcia, Roberto

doi:10.1080/1828051x.2022.2056527

Cited by 8 publications

(6 citation statements)

References 46 publications

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“…To perform the MDA assay in plasma, it was homogenized with a malondialdehyde (MDA) lysis buffer and a thiobarbituric acid (TBA) solution was added to each sample and incubated at 95 °C for 60 min [ 51 ]. Then, each reaction mixture was placed into a 96-well plate to measure the absorbance at 532 nm using a Glomax Multi Detection System (Promega, Madison, WI, USA).…”

Section: Methodsmentioning

confidence: 99%

Effects of Dietary Supplementations of Vitamin C, Organic Selenium, Betaine, and Pomegranate Peel on Alleviating the Effect of Heat Stress on Growing Rabbits

Abu Hafsa,

Centoducati,

Hassan

et al. 2024

Animals

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The aim of this study was to investigate the biological activity and sustainable biorefinery development of vitamin C, organic selenium, betaine, and pomegranate peel on the performance, cecal fermentation, and antioxidant status of growing rabbits to alleviate the negative impacts of heat stress. A total of 105 male rabbits at 35 days old with an average weight of 752.55 ± 27.56 g were randomly assigned to five groups (21 rabbits in each). The experimental dietary groups included a control group fed a basal diet without additives (control group) and four treated groups, A, B, C, and D, fed a basal diet supplemented with either a 1000 mg vitamin C/kg diet, a 25 mg organic selenium/kg diet, a 1000 mg betaine/kg diet, or a 20 g pomegranate peel/kg diet, respectively. No negative group (not heat-stressed) was included in the trial. Rabbits given A, B, C, and D supplements showed a significant increase (p < 0.05) in growth performance, nutrient digestion, concentration of total volatile fatty acids (VFA), acetic, and propionic acids, and total bacterial count, and a significant decrease (p < 0.05) in NH3-N concentration, Enterococcus, coliforms, and E. coli counts in the cecum of rabbits. Total protein, albumin, globulin, superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and glutathione peroxidase (GPx) were significantly (p < 0.05) higher in groups supplemented with A, B, C, and D supplements compared to those in the control group. Total cholesterol, triglycerides, creatinine, urea, and malondialdehyde (MDA) were significantly (p < 0.05) lower in groups supplemented with A, B, C, and D supplements compared to those in the control group. In conclusion, the finding showed that the supplementation of vitamin C, organic selenium, betaine, and pomegranate peel played a significant role in alleviating heat stress and had a further beneficial impact on rabbit performance, cecal fermentation and microbiota, and antioxidant status. However, betaine supplementation had a superior positive effect on the nutritional and physiological profile of heat-stressed rabbits.

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Section: Methodsmentioning

confidence: 99%

Effects of Dietary Supplementations of Vitamin C, Organic Selenium, Betaine, and Pomegranate Peel on Alleviating the Effect of Heat Stress on Growing Rabbits

Abu Hafsa,

Centoducati,

Hassan

et al. 2024

Animals

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“…In this regard, Damiano et al further investigated the mechanism of action and proved RLE's ability to prevent renal damage during the progression of nephropathy by inhibiting the increase in NOX4 [78]. In a recent article, RLE was suggested as a dietary supplement to protect lambs from oxidative stress [79], supporting the idea of considering RLE as a dietary feed supplement to counteract the adverse effects of OTA poisoning.…”

Section: Red Orange and Lemon Extractmentioning

confidence: 99%

Ochratoxin A and Kidney Oxidative Stress: The Role of Nutraceuticals in Veterinary Medicine—A Review

Longobardi

Ferrara

Andretta

et al. 2022

Toxins

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The problem of residues of toxic contaminants in food products has assumed considerable importance in terms of food safety. Naturally occurring contaminants, such as mycotoxins, are monitored routinely in the agricultural and food industries. Unfortunately, the consequences of the presence of mycotoxins in foodstuffs are evident in livestock farms, where both subacute and chronic effects on animal health are observed and could have non-negligible effects on human health. Ochratoxin A (OTA) is a common mycotoxin that contaminates food and feeds. Due to its thermal stability, the eradication of OTA from the food chain is very difficult. Consequently, humans and animals are frequently exposed to OTA in daily life. In this review article, we will devote time to highlighting the redox-based nephrotoxicity that occurs during OTA intoxication. In the past few decades, the literature has improved on the main molecules and enzymes involved in the redox signaling pathway as well as on some new antioxidant compounds as therapeutic strategies to counteract oxidative stress. The knowledge shown in this work will address the use of nutraceutical substances as dietary supplements, which would in turn improve the prophylactic and pharmacological treatment of redox-associated kidney diseases during OTA exposure, and will attempt to promote animal feed supplementation.

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“…Wang et al [ 119 ] found that the supplementation of wheat bran feruloyl oligosaccharides at 100 and 200 mg/kg of feed in the diet of lambs improved ADG, the feed conversion rate, serum T-AOC levels, antioxidant enzyme activity, and glutathione content, while it decreased ruminal NH3-N content. Damiano et al [ 120 ] reported that supplementing 90 mg/kg of red orange and lemon extract to the diet regimen of lambs improved the activities of SOD, CAT, and GPx in the plasma, while it decreased the levels of MDA, 8-hydroxy-2′-deoxyguanosine, IL-1β, and IL-6 in the plasma. Beck et al [ 121 ] found that the addition of Lactobacillus fermented plant extracts with terrestrial plant extracts (chicory, plantain, lucerne, and leaf dock) at 10 mL/d to the diet of ewes did not affect the plasma levels of the total antioxidant status or the GPx of the ewes, but it did improve the total antioxidant status level and decrease GPx activity in the plasma of their lambs, indicating that the plant extracts increased the maternal transmission of antioxidants from the ewes to their offspring.…”

Section: Application Of Plant Extracts In Ruminantsmentioning

confidence: 99%

Advances in the Application of Phytogenic Extracts as Antioxidants and Their Potential Mechanisms in Ruminants

Piao

Zhang

et al. 2023

Antioxidants

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Under current breeding conditions, multiple stressors are important challenges facing animal husbandry in achieving animal wellbeing. For many years, the use of antibiotics has been a social concern in the livestock industry. With the implementation of the non-antibiotics policy, there is an urgent need to find relevant technologies and products to replace antibiotics and to solve the problem of disease prevention during animal growth. Phytogenic extracts have the unique advantages of being natural and extensive sources, having a low residue, and being pollution-free and renewable. They can relieve the various stresses, including oxidative stress, on animals and even control their inflammation by regulating the signaling pathways of proinflammatory cytokines, improving animal immunity, and improving the structure of microorganisms in the gastrointestinal tract, thereby becoming the priority choice for improving animal health. In this study, we reviewed the types of antioxidants commonly used in the livestock industry and their applicable effects on ruminants, as well as the recent research progress on their potential mechanisms of action. This review may provide a reference for further research and for the application of other phytogenic extracts and the elucidation of their precise mechanisms of action.

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Red orange and lemon extract preserve from oxidative stress, DNA damage and inflammatory status in lambs

Cited by 8 publications

References 46 publications

Effects of Dietary Supplementations of Vitamin C, Organic Selenium, Betaine, and Pomegranate Peel on Alleviating the Effect of Heat Stress on Growing Rabbits

Effects of Dietary Supplementations of Vitamin C, Organic Selenium, Betaine, and Pomegranate Peel on Alleviating the Effect of Heat Stress on Growing Rabbits

Ochratoxin A and Kidney Oxidative Stress: The Role of Nutraceuticals in Veterinary Medicine—A Review

Advances in the Application of Phytogenic Extracts as Antioxidants and Their Potential Mechanisms in Ruminants

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