Red wine polyphenols correct vascular function injured by chronic carbon tetrachloride intoxication

Abstract

Abstract. The aim of the study was to evaluate the effect of red wine polyphenols extract Provinols TM on the development of cardiovascular injury in the model of carbon tetrachloride (CCl 4 ) intoxication. We followed the thoracic aorta vasoactivity and left ventricle nitric oxide (NO) synthase activity in male Wistar rats. In the preventive experiment lasting for 12 weeks the control group, the group receiving CCl 4 (0.5 ml/kg) two times a week subcutaneously, the group receiving Provinols TM (30 mg/kg/day) in drinking water and the group receiving CCl 4 +Provinols TM was used. In the recovery experiment, the initial 12 weeks of CCl 4 treatment were followed by 3 weeks of spontaneous recovery or recovery with Provinols TM . CCl 4 -intoxication resulted in the injury of vasoactivity which was demonstrated by the inhibition of acetylcholine-induced relaxation as well as noradrenaline-induced contraction. In the preventive as well as recovery experiment administration of polyphenols refreshed endotheliumdependent relaxant response and normalized inhibited contraction to adrenergic stimuli. Provinols TM treatment significantly increased NO-synthase activity in all groups. The results revealed beneficial effects of red wine polyphenols on vascular function injured by chronic CCl 4 intoxication. The correction of endothelial function seems to be attributed to the activation of NO pathway by polyphenols.