Redefining nitric oxide production in legume nodules through complementary insights from electron paramagnetic resonance spectroscopy and specific fluorescent probes

Calvo-Beguería, Laura; Rubio, Maria C.; Martínez, Jesús I.; Pérez-Rontomé, Carmen; Delgado, Manuel Lorenzo; Bedmar, Eulogio J.; Becana, Manuel

doi:10.1093/jxb/ery159

Cited by 32 publications

(31 citation statements)

References 42 publications

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“…However, additional forms of Lb have been detected under certain developmental or stressful conditions. The presence of nitrosyl‐Lb in soybean intact nodules was reported using electron paramagnetic resonance in healthy nodules (Mathieu et al , ) and in nodules deficient in the bacteroid denitrification enzymes NirK or Nor (Calvo‐Begueria et al , ), especially in response to flooding conditions (Sánchez et al , ). On the other hand, green pigments derived from Lbs were observed in senescing soybean nodules (Jun et al , ,b) and almost 20 years later identified as nitri‐Lbs, unveiling the generation of nitrating reactive compounds in nodules (Navascués et al , ).…”

Section: Globins In Symbiosismentioning

confidence: 92%

Plant hemoglobins: a journey from unicellular green algae to vascular plants

et al. 2020

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Globins (Glbs) are widely distributed in archaea, bacteria and eukaryotes. They can be classified into proteins with 2/2 or 3/3 a-helical folding around the heme cavity. Both types of Glbs occur in green algae, bryophytes and vascular plants. The Glbs of angiosperms have been more intensively studied, and several protein structures have been solved. They can be hexacoordinate or pentacoordinate, depending on whether a histidine is coordinating or not at the sixth position of the iron atom. The 3/3 Glbs of class 1 and the 2/2 Glbs (also called class 3 in plants) are present in all angiosperms, whereas the 3/3 Glbs of class 2 have been only found in early angiosperms and eudicots. The three Glb classes are expected to play different roles. Class 1 Glbs are involved in hypoxia responses and modulate NO concentration, which may explain their roles in plant morphogenesis, hormone signaling, cell fate determination, nutrient deficiency, nitrogen metabolism and plant-microorganism symbioses. Symbiotic Glbs derive from class 1 or class 2 Glbs and transport O 2 in nodules. The physiological roles of class 2 and class 3 Glbs are poorly defined but could involve O 2 and NO transport and/or metabolism, respectively. More research is warranted on these intriguing proteins to determine their non-redundant functions.

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Section: Globins In Symbiosismentioning

confidence: 92%

Plant hemoglobins: a journey from unicellular green algae to vascular plants

et al. 2020

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“…NO production also has been reported in mature N 2 -fixing nodules, especially in infected rhizobia-harbouring cells (Calvo-Begueria et al, 2018;Horchani et al, 2011) and during nodule senescence (Cam et al, 2012;Fukudome et al, 2019). Accumulating evidence also supports a multifaceted role for NO during N 2 -fixation.…”

Section: Nitric Oxide Plays Pivotal Roles During Pathogen Defence Amentioning

confidence: 63%

Physiological significance of pedospheric nitric oxide for root growth, development and organismic interactions

Wendehenne

Philippot

et al. 2020

Plant Cell & Environment

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Nitric oxide (NO) is essential for plant growth and development, as well as interactions with abiotic and biotic environments. Its importance for multiple functions in plants means that tight regulation of NO concentrations is required. This is of particular significance in roots, where NO signalling is involved in processes, such as root growth, lateral root formation, nutrient acquisition, heavy metal homeostasis, symbiotic nitrogen fixation and root–mycorrhizal fungi interactions. The NO signal can also be produced in high levels by microbial processes in the rhizosphere, further impacting root processes. To explore these interesting interactions, in the present review, we firstly summarize current knowledge of physiological processes of NO production and consumption in roots and, thereafter, of processes involved in NO homeostasis in root cells with particular emphasis on root growth, development, nutrient acquisition, environmental stresses and organismic interactions.

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“…NO binds to the Fe 2+ of leghemoglobin and forms an Lb 2+ NO nitrosyl complex (left). Calvo-Begueria et al (2018) have demonstrated that this complex can be detected by EPR spectroscopy in intact soybean nodules, allowing a direct monitoring of NO production. Nodules containing Lb 2+ NO show spectra with a clear diagnostic signal in the range of 320–345 mT (right; see Calvo-Begueria et al , 2018 ).…”

Section: Breakthrough Methodologymentioning

confidence: 99%

“…The paper presented by Laura Calvo-Begueria and colleagues describes a method that enables detection of NO in vivo ( Calvo-Begueria et al , 2018 ). They investigated the formation of the nitrosyl–leghemoglobin complex (Lb 2+ NO) and the production of NO in legume nodules using EPR spectroscopy and the fluorescent specific dye 4,5-diaminofluorescein diacetate (DAF-2 DA), respectively.…”

Section: Breakthrough Methodologymentioning

confidence: 99%