Redox regulation of ascorbate and glutathione by a chloroplastic dehydroascorbate reductase is required for high-light stress tolerance in <i>Arabidopsis</i>

Noshi, Masahiro; Hatanaka, Risa; Tanabe, Noriaki; Terai, Yusuke; Maruta, Takanori; Shigeoka, Shigeru

doi:10.1080/09168451.2015.1135042

Cited by 55 publications

(37 citation statements)

References 48 publications

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“…A minor contribution to the cytosolic activity would come from DHAR2. This is consistent with two recent reports on single dhar mutants (Noshi et al, 2016(Noshi et al, , 2017 but in contrast to an earlier analysis of dhar2 (Yoshida et al, 2006).…”

Section: Discussionsupporting

confidence: 93%

“…We cannot discount a role for DHARs in maintaining ascorbate in other conditions. For example, changes in ascorbate redox state were reported in dhar1 and dhar2 single mutants subjected to high light (Noshi et al, 2016(Noshi et al, , 2017. However, these effects were relatively slight.…”

Section: Arabidopsis Can Maintain Leaf Ascorbate Pools and Redox Statmentioning

confidence: 99%

“…Single mutants for both genes were reported to show modified ascorbate and glutathione redox states following photooxidative stress (Noshi et al, 2017). A similar study of DHAR3 also reported decreased activity and altered responses to photooxidative stress (Noshi et al, 2016).…”

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confidence: 94%

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Cytosolic and Chloroplastic DHARs Cooperate in Oxidative Stress-Driven Activation of the Salicylic Acid Pathway

Rahantaniaina

Li²,

Châtel-Innocenti³

et al. 2017

Plant Physiol.

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The complexity of plant antioxidative systems gives rise to many unresolved questions. One relates to the functional importance of dehydroascorbate reductases (DHARs) in interactions between ascorbate and glutathione. To investigate this issue, we produced a complete set of loss-of-function mutants for the three annotated Arabidopsis (Arabidopsis thaliana) DHARs. The combined loss of DHAR1 and DHAR3 expression decreased extractable activity to very low levels but had little effect on phenotype or ascorbate and glutathione pools in standard conditions. An analysis of the subcellular localization of the DHARs in Arabidopsis lines stably transformed with GFP fusion proteins revealed that DHAR1 and DHAR2 are cytosolic while DHAR3 is chloroplastic, with no evidence for peroxisomal or mitochondrial localizations. When the mutations were introduced into an oxidative stress genetic background (cat2), the dhar1 dhar2 combination decreased glutathione oxidation and inhibited cat2-triggered induction of the salicylic acid pathway. These effects were reversed in cat2 dhar1 dhar2 dhar3 complemented with any of the three DHARs. The data suggest that (1) DHAR can be decreased to negligible levels without marked effects on ascorbate pools, (2) the cytosolic isoforms are particularly important in coupling intracellular hydrogen peroxide metabolism to glutathione oxidation, and (3) DHAR-dependent glutathione oxidation influences redox-driven salicylic acid accumulation.

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Section: Discussionsupporting

confidence: 93%

Section: Arabidopsis Can Maintain Leaf Ascorbate Pools and Redox Statmentioning

confidence: 99%

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Cytosolic and Chloroplastic DHARs Cooperate in Oxidative Stress-Driven Activation of the Salicylic Acid Pathway

Rahantaniaina

Li²,

Châtel-Innocenti³

et al. 2017

Plant Physiol.

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“…However, the ROS can be scavenged by plant antioxidant defense system consisting of antioxidant compounds and enzymes (Apel and Hirt, 2004; Suzuki et al, 2012). The ascorbate (ASA)–glutathione (GSH) cycle is one of the main antioxidant systems in plants to keep ROS under control being, which involves a lot of critical antioxidant enzymes in a series of cyclic reactions to detoxify H 2 O 2 (de Sousa et al, 2016; Noshi et al, 2016). One of the antioxidant enzymes is peroxidase (POD), which can detoxify H 2 O 2 by oxidizing ascorbate.…”

Section: Discussionmentioning

confidence: 99%

Functional and Integrative Analysis of the Proteomic Profile of Radish Root under Pb Exposure

Wang

Tang

et al. 2016

Front. Plant Sci.

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Lead (Pb) is one of the most abundant heavy metal (HM) pollutants, which can penetrate the plant through the root and then enter the food chain causing potential health risks for human beings. Radish is an important root vegetable crop worldwide. To investigate the mechanism underlying plant response to Pb stress in radish, the protein profile changes of radish roots respectively upon Pb(NO3)2 at 500 mg L−1(Pb500) and 1000 mg L−1(Pb1000), were comprehensively analyzed using iTRAQ (Isobaric Tag for Relative and Absolute Quantification). A total of 3898 protein species were successfully detected and 2141 were quantified. Among them, a subset of 721 protein species were differentially accumulated upon at least one Pb treatment, and 135 ones showed significantly abundance changes under both two Pb-stressed conditions. Many critical protein species related to protein translation, processing, and degradation, reactive oxygen species (ROS) scavenging, photosynthesis, and respiration and carbon metabolism were successfully identified. Gene Ontology (GO) and pathway enrichment analysis of the 135 differential abundance protein species (DAPS) revealed that the overrepresented GO terms included “cell wall,” “apoplast,” “response to metal ion,” “vacuole,” and “peroxidase activity,” and the critical enriched pathways were involved in “citric acid (TCA) cycle and respiratory electron transport,” “pyruvate metabolism,” “phenylalanine metabolism,” “phenylpropanoid biosynthesis,” and “carbon metabolism.” Furthermore, the integrative analysis of transcriptomic, miRNA, degradome, metabolomics and proteomic data provided a strengthened understanding of radish response to Pb stress at multiple levels. Under Pb stress, many key enzymes (i.e., ATP citrate lyase, Isocitrate dehydrogenase, fumarate hydratase and malate dehydrogenase) involved in the glycolysis and TCA cycle were severely affected, which ultimately cause alteration of some metabolites including glucose, citrate and malate. Meanwhile, a series of other defense responses including ascorbate (ASA)–glutathione (GSH) cycle for ROS scavenging and Pb-defense protein species (glutaredoxin, aldose 1-epimerase malate dehydrogenase and thioredoxin), were triggered to cope with Pb-induced injuries. These results would be helpful for further dissecting molecular mechanism underlying plant response to HM stresses, and facilitate effective management of HM contamination in vegetable crops by genetic manipulation.

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“…() that mutants deficient in pyrroline‐5‐carboxylate synthase‐1, having lower proline content, showed reduced activity of the ascorbate‐glutathione cycle, which is an important ROS scavenging pathway (Foyer and Noctor, ; Noshi et al. , ). The higher proline content in L er together with its faster association with the chloroplasts constitutes a clear advantage of the wildtype and may, at least in part, explain the light sensitive phenotype of gin2‐1 .…”

Section: Discussionmentioning

confidence: 99%

Subcellular dynamics of proteins and metabolites under abiotic stress reveal deferred response of the Arabidopsis thaliana hexokinase‐1 mutant gin2‐1 to high light

et al. 2019

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Summary Stress responses in plants imply spatio‐temporal changes in enzymes and metabolites, including subcellular compartment‐specific re‐allocation processes triggered by sudden changes in environmental parameters. To investigate interactions of primary metabolism with abiotic stress, the gin2‐1 mutant, defective in the sugar sensor hexokinase 1 (HXK1) was compared with its wildtype Landsberg erecta (Ler) based on time resolved, compartment‐specific metabolome and proteome data obtained over a full diurnal cycle. The high light sensitive gin2‐1 mutant was substantially delayed in subcellular re‐distribution of metabolites upon stress, and this correlated with a massive reduction in proteins belonging to the ATP producing electron transport chain under high light, while fewer changes occurred in the cold. In the wildtype, compounds specifically protecting individual compartments could be identified, e.g., maltose and raffinose in plastids, myo‐inositol in mitochondria, but gin2‐1 failed to recruit these substances to the respective compartments, or responded only slowly to high irradiance. No such delay was obtained in the cold. At the whole cell level, concentrations of the amino acids, glycine and serine, provided strong evidence for an important role of the photorespiratory pathway during stress exposure, and different subcellular allocation of serine may contribute to the slow growth of the gin2‐1 mutant under high irradiance.

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Redox regulation of ascorbate and glutathione by a chloroplastic dehydroascorbate reductase is required for high-light stress tolerance in Arabidopsis

Cited by 55 publications

References 48 publications

Cytosolic and Chloroplastic DHARs Cooperate in Oxidative Stress-Driven Activation of the Salicylic Acid Pathway

Cytosolic and Chloroplastic DHARs Cooperate in Oxidative Stress-Driven Activation of the Salicylic Acid Pathway

Functional and Integrative Analysis of the Proteomic Profile of Radish Root under Pb Exposure

Subcellular dynamics of proteins and metabolites under abiotic stress reveal deferred response of the Arabidopsis thaliana hexokinase‐1 mutant gin2‐1 to high light

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