Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates

Cordina, Nicole M.; Sayyadi, Nima; Parker, Lindsay M.; Everest‐Dass, Arun; Brown, Louise J.; Packer, Nicolle H.

doi:10.1038/s41598-018-22702-1

Cited by 56 publications

(42 citation statements)

References 40 publications

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“…Background autofluorescence is a major concern in the bio-imaging of cells [ 48 ]. Polystyrene well plates are frequently used but have been reported to present high autofluorescence in the range of 300–400 nm [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

A Medium-Throughput System for In Vitro Oxidative Stress Assessment in IPEC-J2 Cells

Ayuso

Cruchten

Ginneken

2020

IJMS

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The feed industry continuously seeks new molecules with antioxidant capacity since oxidative stress plays a key role in intestinal health. To improve screening of new antioxidants, this study aims to set up an assay to assess oxidative stress in the porcine small intestinal epithelial cell line IPEC-J2 using plate-reader-based analysis of fluorescence. Two oxidants, H2O2 and menadione, were tested at 1, 2 and 3 mM and 100, 200 and 300 µM, respectively. Trolox (2 mM) was used as the reference antioxidant and the probe CM-H2DCFDA was used to indicate intracellular oxidative stress. Cell culture, reactive oxygen species (ROS) production and assessment conditions were optimized to detect a significant ROS accumulation that could be counteracted by pre-incubation with trolox. Menadione (200 µM) reproducibly increased ROS levels, H2O2 failed to do so. Trolox significantly decreased intracellular ROS levels in menadione (200 µM)-exposed cells in a consistent way. The system was further used to screen different concentrations of the commercially available antioxidant ELIFE®. Concentrations between 100 and 200 ppm protected best against intracellular ROS accumulation. In conclusion, the combination of CM-H2DCFDA fluorescence analysis by a plate-reader, trolox as a reference antioxidant and 200 µM of menadione as a stressor agent, provides a replicable and reliable medium-throughput setup for the evaluation of intracellular oxidative stress in IPEC-J2 cells.

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Section: Discussionmentioning

confidence: 99%

A Medium-Throughput System for In Vitro Oxidative Stress Assessment in IPEC-J2 Cells

Ayuso

Cruchten

Ginneken

2020

IJMS

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“…As microscopy techniques mature, so do the quality of FND particles at smaller sizes, opening up new capabilities 32 . For example, 30 nm particles allowed staining of E-selectin inflamed cells, increasing cellular detection by 40× 106 . Even imaging of NV containing particles with sizes down to 20 nm has been readily demonstrated by laser excitation (Fig.…”

Section: Applications Of Fluorescent Diamond Particlesmentioning

confidence: 99%

Review Article: Synthesis, properties, and applications of fluorescent diamond particles

Shenderova

Shames

Nunn

et al. 2019

Journal of Vacuum Science &Amp; Technology B, Nanotechnology and Microelectronics: Materials, Processing, Measurement, and Phen

140

146

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Diamond particles containing color centers—fluorescent crystallographic defects embedded within the diamond lattice—outperform other classes of fluorophores by providing a combination of unmatched photostability, intriguing coupled magneto-optical properties, intrinsic biocompatibility, and outstanding mechanical and chemical robustness. This exceptional combination of properties positions fluorescent diamond particles as unique fluorophores with emerging applications in a variety of fields, including bioimaging, ultrasensitive metrology at the nanoscale, fluorescent tags in industrial applications, and even potentially as magnetic resonance imaging contrast agents. However, production of fluorescent nanodiamond (FND) is nontrivial, since it requires irradiation with high-energy particles to displace carbon atoms and create vacancies—a primary constituent in the majority color centers. In this review, centrally focused on material developments, major steps of FND production are discussed with emphasis on current challenges in the field and possible solutions. The authors demonstrate how the combination of fluorescent spectroscopy and electron paramagnetic resonance provides valuable insight into the types of radiation-induced defects formed and their evolution upon thermal annealing, thereby guiding FND performance optimization. A recent breakthrough process allowing for production of fluorescent diamond particles with vibrant blue, green, and red fluorescence is also discussed. Finally, the authors conclude with demonstrations of a few FND applications in the life science arena and in industry.

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“…The most important applications and properties of nanodiammonds in infection management are presented in Figure 2. NDs harbor a nitrogen-vacancy enabling them to emit fluorescence when illuminated [9,45,46]; moreover, their magnetic properties can be used as a contrast agent for MRI [46]. Few studies have been carried out in the field of infectious diseases regarding ND diagnosis.…”

Section: Nanodiamonds In Infection Diagnosismentioning

confidence: 99%

Applications of Nanodiamonds in the Detection and Therapy of Infectious Diseases

2019

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We are constantly exposed to infectious diseases, and they cause millions of deaths per year. The World Health Organization (WHO) estimates that antibiotic resistance could cause 10 million deaths per year by 2050. Multidrug-resistant bacteria are the cause of infection in at least one in three people suffering from septicemia. While antibiotics are powerful agents against infectious diseases, the alarming increase in antibiotic resistance is of great concern. Alternatives are desperately needed, and nanotechnology provides a great opportunity to develop novel approaches for the treatment of infectious diseases. One of the most important factors in the prognosis of an infection caused by an antibiotic resistant bacteria is an early and rigorous diagnosis, jointly with the use of novel therapeutic systems that can specifically target the pathogen and limit the selection of resistant strains. Nanodiamonds can be used as antimicrobial agents due to some of their properties including size, shape, and biocompatibility, which make them highly suitable for the development of efficient and tailored nanotherapies, including vaccines or drug delivery systems. In this review, we discuss the beneficial findings made in the nanodiamonds field, focusing on diagnosis and treatment of infectious diseases. We also highlight the innovative platform that nanodiamonds confer for vaccine improvement, drug delivery, and shuttle systems, as well as their role in the generation of faster and more sensitive clinical diagnosis.

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Reduced background autofluorescence for cell imaging using nanodiamonds and lanthanide chelates

Cited by 56 publications

References 40 publications

A Medium-Throughput System for In Vitro Oxidative Stress Assessment in IPEC-J2 Cells

A Medium-Throughput System for In Vitro Oxidative Stress Assessment in IPEC-J2 Cells

Review Article: Synthesis, properties, and applications of fluorescent diamond particles

Applications of Nanodiamonds in the Detection and Therapy of Infectious Diseases

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