Reduced extracellular phagocyte oxidative activity, antioxidant level changes and increased oxidative damage in healthy human blood as a function of age

Alexandrova, Margarita L.; Bochev, Petyo G.

doi:10.1007/s11357-008-9085-9

Cited by 13 publications

(7 citation statements)

References 38 publications

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“…These prooxidant factors (AGE's) specially Nεcarboxymethyl-lysine may also contribute to our observed changes in sialic acid level and lipid peroxidation levels during aging [14]. A decrease in total plasma total antioxidant capacity as a function of human age has been reported by us and by other workers [22,39] however in a significant finding we showed that the decrease of antioxidant potential was sharper in case of Indian population. Our present observations corroborate earlier reports.…”

Section: Discussionsupporting

confidence: 65%

Erythrocyte Sialic Acid Content during Aging in Humans: Correlation with Markers of Oxidative Stress

2012

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Sialic acids are substituted neuraminic acid derivatives which are typically found at the outermost end of glycan chains on the membrane in all cell types. The role of erythrocyte membrane sialic acids during aging has been established however the relationship between sialic acid and oxidative stress is not fully understood. The present work was undertaken to analyze the relationship between erythrocyte membrane sialic acid with its plasma level, membrane and plasma lipid hydroperoxide levels and plasma total antioxidant capacity. Results show that sialic acid content decreases significantly (P < 0.001) in RBC membrane (r = −0.901) and increases in plasma (r = 0.860) as a function of age in humans. Lipid peroxidation measured in the form of hydroperoxides increases significantly (P < 0.001) in plasma (r = 0.830) and RBC membranes (r = 0.875) with age in humans. The Trolox Equivalent Total Antioxidant Capacity (TETAC) of plasma was found to be significantly decreased (P < 0.001, r = −0.844). We observe significant correlations between decrease of erythrocyte membrane sialic acid and plasma lipid hydroperoxide and TETAC. Based on the observed correlations, we hypothesize that increase in oxidative stress during aging may influence the sialic acid decomposition from membrane thereby altering the membrane configuration affecting many enzymatic and transporter activities. Considering the importance of plasma sialic acid as a diagnostic parameter, it is important to establish age-dependent reference.

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Section: Discussionsupporting

confidence: 65%

Erythrocyte Sialic Acid Content during Aging in Humans: Correlation with Markers of Oxidative Stress

2012

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“…In addition, the age of donors was identified as a further important parameter of antioxidant enzyme activity . Concerning CAT, our data partly support this relationship, but published work on CAT enzyme level is generally inconsistent, and results were mainly determined using the blood of a much higher number of healthy human volunteers . SOD2 activity, however, was significantly and inversely correlated with the age of donors in normal colon tissue.…”

Section: Discussionsupporting

confidence: 69%

Butyrate modulates antioxidant enzyme expression in malignant and non‐malignant human colon tissues

Jahns

Wilhelm

Jablonowski

et al. 2014

Molecular Carcinogenesis

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The induction of antioxidant enzymes is an important mechanism in colon cancer chemoprevention, but the response of human colon tissue to butyrate, a gut fermentation product derived from dietary fiber, remains largely unknown. Therefore, our study investigated the effect of a butyrate treatment on catalase (CAT) and superoxide dismutase (SOD2) in matched human colon tissues of different transformation stages (n = 3-15 in each group) ex vivo. By performing quantitative real-time PCR, Western blot, and spectrophotometric measurements, we found an increase in SOD2 at expression and activity level in colonic adenocarcinomas (mRNA: 1.96-fold; protein: 1.41-fold, activity: 1.8-fold; P < 0.05). No difference was detectable for CAT between normal, adenoma, and carcinoma colon tissues. Treatment of normal colon epithelium (12 h) with a physiologically relevant concentration of butyrate (10 mM) resulted in a significant increase (P < 0.05) in CAT mRNA (1.24-fold) and protein (1.39-fold), without affecting the enzymatic activity. Consequently, preliminary experiments failed to show any protective effect of butyrate against H2 O2 -mediated DNA damage. Despite a significantly lowered SOD2 transcript (0.51-fold, P < 0.01) and, to a lesser extent, protein level (0.86-fold) after butyrate exposure of normal colon cells, the catalytic activity was significantly enhanced (1.19-fold, P < 0.05), suggesting an increased protection against tissue superoxide radicals. In malignant tissues, greater variations in response to butyrate were observed. Furthermore, both enzymes showed an age-dependent decrease in activity in normal colon epithelium (CAT: r = -0.49, P = 0.09; SOD2: r = -0.58, P = 0.049). In conclusion, butyrate exhibited potential antioxidant features ex vivo but cellular consequences need to be investigated more in depth.

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“…The increased oxidative/nitrosative stresses found in aging animals and humans have been linked to peroxidative damage to lipids, DNA, proteins and carbohydrates as well increased levels of SOD and CAT, and impairment of the total antioxidant capacity (İnal et al 2001;Lasheras et al 2002;Mutlu-Türkoǧlu et al 2003;Rizvi and Maurya 2007;Dayhoff-Brannigan et al 2008;Alexandrova and Bochev 2009). In fact, excessive production of hydrogen peroxide is very harmful to aging muscles.…”

Section: Antioxidant Capacity and Healthy Aging: Does Exercise Matter?mentioning

confidence: 99%

Exercise modulation of total antioxidant capacity (TAC): towards a molecular signature of healthy aging

Ferrari

2011

Frontiers in Life Science

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The objective of this article was to review the biochemical basis of the total antioxidant capacity (TAC) test and its applications to exercise science and promotion of healthy aging. Measurement of TAC allows evaluation of the antioxidant content of muscle, heart and other exercise-target organs. Acute non-regular physical exercise and body exposition to higher altitude have been associated with a considerable decrease of blood TAC. However, regular practice of physical activity and exercise is associated with improved antioxidant response increasing TAC levels. TAC methodologies did not address lipophylic antioxidants and have some other limitations. However, TAC is useful as a complementary test for the evaluation of antiperoxidation assays and blood, erythrocyte and cytosolic antioxidant enzyme assays (glutathion reductase, glutathion peroxidase, superoxide dismutase and catalase). Exercise induces both molecular antioxidant and hormetic responses which have been suggested to be linked with a healthy aging.

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Reduced extracellular phagocyte oxidative activity, antioxidant level changes and increased oxidative damage in healthy human blood as a function of age

Cited by 13 publications

References 38 publications

Erythrocyte Sialic Acid Content during Aging in Humans: Correlation with Markers of Oxidative Stress

Erythrocyte Sialic Acid Content during Aging in Humans: Correlation with Markers of Oxidative Stress

Butyrate modulates antioxidant enzyme expression in malignant and non‐malignant human colon tissues

Exercise modulation of total antioxidant capacity (TAC): towards a molecular signature of healthy aging

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