Reduced inflammatory activity of RAW 264.7 macrophages on titania nanotube modified Ti surface

Neacsu, Patricia; Mazare, Anca; Cîmpean, Anișoara; Park, Jung; Costache, Marieta; Schmuki, Patrik; Demetrescu, Ioana

doi:10.1016/j.biocel.2014.09.006

Cited by 103 publications

(90 citation statements)

References 39 publications

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“…30 Our group has recently reported that TiO 2 nanotubes exhibited a significant reduction in pro-inflammatory activity of LPSstimulated RAW 264.7 macrophages with respect to cytokine/ chemokine gene expression and protein secretion, and nitric oxide (NO) release, as compared with titanium flat surface. 33 This attenuation effect of titania nanotubes was sustainable in long-term culture, as remarked by the decrease in the percentage of macrophage fusion into multinucleated cells at 7 days postseeding, as compared with commercial pure titanium (cpTi). More than that, under LPS-treatment, these cells adopted the morphology of foreign body giant cells (a hallmark histological feature of the chronic inflammation and foreign body response) only on the cpTi surface.…”

Section: Introductionmentioning

confidence: 91%

“…33 Briefly, the nanotubes are grown in a twoelectrode configuration (sample -anode, Pt grid -cathode) in …”

Section: Samples Preparation and Characterizationmentioning

confidence: 99%

“…33 Before anodization, the Ti foils were cleaned by ultrasonication (acetone, ethanol, and water -for 5 minutes each). The morphology and properties of the samples were investigated using scanning electron microscopy (Hitachi FE-SEM S4800), atomic force microscopy (A.P.E.…”

Section: −1mentioning

confidence: 99%

“…4,[26][27][28] Studies investigating the surface-dependent inflammatory responses have shown that nanostructured surfaces are able to mitigate the activation of macrophages by reducing the secretion of the pro-inflammatory mediators. [29][30][31][32][33][34] Moreover, surface nanostructuring of Ti (or Ti alloys) can be easily performed by electrochemical anodization, resulting in nanotubular structures directly coated on the Ti substrate. 35 For TiO 2 nanotubes, the activation of macrophages was shown to depend on the nanotopography, that is the diameter of the nanotubes is the key morphological parameter influencing inflammatory responses (from diameters ranging from 30 to 100 nm, the 70 nm showed the weakest inflammatory response).…”

Section: Introductionmentioning

confidence: 99%

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Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways

Neacsu¹,

Mazare²,

Schmuki³

et al. 2015

IJN

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Biomaterial implantation in a living tissue triggers the activation of macrophages in inflammatory events, promoting the transcription of pro-inflammatory mediator genes. The initiation of macrophage inflammatory processes is mainly regulated by signaling proteins of mitogen-activated protein kinase (MAPK) and by nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathways. We have previously shown that titania nanotubes modified Ti surfaces (Ti/TiO 2 ) mitigate the immune response, compared with flat Ti surfaces; however, little is known regarding the underlying mechanism. Therefore, the aim of this study is to investigate the mechanism(s) by which this nanotopography attenuates the inflammatory activity of macrophages. Thus, we analyzed the effects of TiO 2 nanotubes on the activation of MAPK and NF-κB signaling pathways in standard and lipopolysaccharide-evoked conditions. Results showed that the Ti/TiO 2 significantly reduce the expression levels of the phosphorylated forms of p38, ERK1/2, c-Jun NH 2 -terminal kinase (JNK), IKKβ, and IkB-α. Furthermore, a significant reduction in the p65 nuclear accumulation on the nanotubular surface was remarked. Following, by using specific MAPK inhibitors, we observed that lipopolysaccharide-induced production of monocyte chemotactic protein-1 and nitric oxide was significantly inhibited on the Ti/TiO 2 surface via p38 and ERK1/2, but not via JNK. However, the selective inhibitor for JNK signaling pathway (SP600125) was effective in reducing tumor necrosis factor alpha release as well as monocyte chemotactic protein-1 and nitric oxide production. Altogether, these data suggest that titania nanotubes can attenuate the macrophage inflammatory response via suppression of MAPK and NF-κB pathways providing a potential mechanism for their anti-inflammatory activity.

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Section: Introductionmentioning

confidence: 91%

“…33 Briefly, the nanotubes are grown in a twoelectrode configuration (sample -anode, Pt grid -cathode) in …”

Section: Samples Preparation and Characterizationmentioning

confidence: 99%

Section: −1mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways

Neacsu¹,

Mazare²,

Schmuki³

et al. 2015

IJN

Self Cite

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“…The in vivo observation showing a reduced inflammatory response with 80 nm semispherical protrusions is in agreement with previous in vitro findings showing that nanotubes with similar size range (70-80 nm) reduce TNF-α, IL-1β, MCP-1, IL-6, and macrophage inflammatory protein 1-alpha (MIP-1α) in primary human monocytes/ macrophages and macrophage cell lines. [30][31][32][33] Interestingly, on the other hand, surfaces with similar nanoscale size range but with a groove shape were found to stimulate the expression/secretion of TNF-α and IL-1β in the murine macrophage RAW264.7 cell line in vitro. 34 Taken together, it is apparent that both the size (micron versus nano) and the shape (eg, tubes versus grooves) are important determinants for macrophage activation.…”

Section: Notesmentioning

confidence: 99%

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

Karazisis¹,

Ballo²,

Petronis³

et al. 2016

IJN

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Purpose Mechanisms governing the cellular interactions with well-defined nanotopography are not well described in vivo. This is partly due to the difficulty in isolating a particular effect of nanotopography from other surface properties. This study employed colloidal lithography for nanofabrication on titanium implants in combination with an in vivo sampling procedure and different analytical techniques. The aim was to elucidate the effect of well-defined nanotopography on the molecular, cellular, and structural events of osseointegration. Materials and methods Titanium implants were nanopatterned (Nano) with semispherical protrusions using colloidal lithography. Implants, with and without nanotopography, were implanted in rat tibia and retrieved after 3, 6, and 28 days. Retrieved implants were evaluated using quantitative polymerase chain reaction, histology, immunohistochemistry, and energy dispersive X-ray spectroscopy (EDS). Results Surface characterization showed that the nanotopography was well defined in terms of shape (semispherical), size (79±6 nm), and distribution (31±2 particles/µm 2 ). EDS showed similar levels of titanium, oxygen, and carbon for test and control implants, confirming similar chemistry. The molecular analysis of the retrieved implants revealed that the expression levels of the inflammatory cytokine, TNF-α , and the osteoclastic marker, CatK , were reduced in cells adherent to the Nano implants. This was consistent with the observation of less CD163-positive macrophages in the tissue surrounding the Nano implant. Furthermore, periostin immunostaining was frequently detected around the Nano implant, indicating higher osteogenic activity. This was supported by the EDS analysis of the retrieved implants showing higher content of calcium and phosphate on the Nano implants. Conclusion The results show that Nano implants elicit less periimplant macrophage infiltration and downregulate the early expression of inflammatory ( TNF -α) and osteoclastic ( CatK ) genes. Immunostaining and elemental analyses show higher osteogenic activity at the Nano implant. It is concluded that an implant with the present range of well-defined nanocues attenuates the inflammatory response while enhancing mineralization during osseointegration.

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Dealing with the Foreign‐Body Response to Implanted Biomaterials: Strategies and Applications of New Materials

Zhang

Chen

Shi

et al. 2020

Adv Funct Materials

176

139

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Foreign‐body response caused by implanted biomaterials seriously impedes the function of implants and is a major obstacle to the development of implantable biomaterials and medical devices. Recent advances in implantable biomaterials and medical devices have provided strategies to resist the foreign‐body response. In this review, the mechanism of the foreign‐body response and conventional strategies to mitigate foreign‐body response is briefly introduced. Then, three types of promising foreign‐body response resisting materials are focused and the advantages, characteristics, and applications of each material are discussed. Finally, prospects are put forward for future development of foreign‐body response resisting materials and current challenges that require in‐depth study.

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Reduced inflammatory activity of RAW 264.7 macrophages on titania nanotube modified Ti surface

Cited by 103 publications

References 39 publications

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo

Dealing with the Foreign‐Body Response to Implanted Biomaterials: Strategies and Applications of New Materials

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Reduced inflammatory activity of RAW 264.7 macrophages on titania nanotube modified Ti surface

Cited by 103 publications

References 39 publications

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-&kappa;B pathways

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-&kappa;B pathways

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and&nbsp;molecular events in vivo

Dealing with the Foreign‐Body Response to Implanted Biomaterials: Strategies and Applications of New Materials

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Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways

Attenuation of the macrophage inflammatory activity by TiO2 nanotubes via inhibition of MAPK and NF-κB pathways

The role of well-defined nanotopography of titanium implants on osseointegration: cellular and molecular events in vivo