Reduced negative surface charge on arterial endothelium of diabetic rats

Havivi, Y.; Yarom, R.

doi:10.1007/bf00264770

Cited by 27 publications

(14 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This modification appeared soon after the induction of disease, and is analogous with that reported for diabetic arterial endothelium [25], myocardial and retinal capillary endothelium [24], and endocardium [26]. The lack of a continuous anionic charge on the luminal surface of the endothelial cells may impede the relationship of the latter with the circulating blood cells (that also have a net negative charged surface), as well as with the circulating plasma macromolecules.…”

Section: Discussionsupporting

confidence: 70%

Alterations of lung structure in experimental diabetes, and diabetes associated with hyperlipidaemia in hamsters

Popov¹,

Simionescu²

1997

Eur Respir J

View full text Add to dashboard Cite

Alterations of lung structure in experimental diabetes, and diabetes associated with hyperlipidaemia in hamsters. D. Popov, M. Simionescu. ©ERS Journals Ltd 1997. ABSTRACT: Since hyperglycaemia is known to affect normal pulmonary physiology and biochemistry and few structure-function correlations have been reported, we designed experiments on hamsters subjected to streptozotocin-induced diabetes or diabetes associated with hyperlipidaemia, and investigated the impact of these conditions on the lung structure.At time intervals ranging 2-24 weeks from the inception of disease (without correcting blood glucose with insulin), the animals were sacrificed, and plasma glucose and cholesterol assayed. The lung was processed for electron microscopy, and the structural changes of the capillary and venular endothelium, of epithelial cells, and interstitium were examined.In diabetic animals, especially after 6 weeks of disease, a gradual narrowing of 35% of the capillaries and ~30% of the alveoli, and hyperplasia of the extracellular matrix, rich in collagen bundles, were observed. Frequently, capillaries contained adherent intravascular macrophages suggestive of an inflammatory process. The capillary endothelium was characterized by numerous plasmalemmal vesicles, often fused, well-developed synthesizing apparatus (endoplasmic reticulum and Golgi complex) and cytoskeleton, and an uneven distribution of the anionic sites on the luminal plasmalemma. The venular endothelium was particularly rich in Weibel-Palade bodies. The alveolar epithelium was often collapsed, compressing surfactant within the airspace. The lung interstitium was apparently enlarged, and the fibroblasts and contractile interstitial cells frequently contained lipid droplets. These alterations were more pronounced and occurred at a faster rate (4 weeks) in diabetes associated with hyperlipidaemia.The structural modifications reported in this study support the functional disturbances observed in association with hyperglycaemia, sustaining the conclusion that the lung is an organ affected by diabetes.

show abstract

Section: Discussionsupporting

confidence: 70%

Alterations of lung structure in experimental diabetes, and diabetes associated with hyperlipidaemia in hamsters

Popov¹,

Simionescu²

1997

Eur Respir J

View full text Add to dashboard Cite

show abstract

“…Cr in the liver reportedly increases significantly while Cr in the kidney decreases in STZ-treated rats [40]; the differences were observed over the 3-week course of the experiment. STZ-treated rats have less Cr in bone 180 days after treatment [41]. After 79 days from the initiation of diabetes, diabetic female rats have been reported to have less Cr in their bones than control females; a similar trend for males was observed [42].…”

Section: Discussionmentioning

confidence: 64%

A comparison of the insulin-sensitive transport of chromium in healthy and model diabetic rats

Clodfelder

Upchurch

Vincent

2004

Journal of Inorganic Biochemistry

View full text Add to dashboard Cite

“…Adhesion was best achieved on Primaria culture plates, which are coated with protonated amine groups and thus assume a positive charge. Because animal cells are characterized by a negative surface charge (17,18), the initial attachment of cells to the plates presumably was attributable to electrostatic interactions.…”

Section: Resultsmentioning

confidence: 99%

Extracellular matrix production and calcium carbonate precipitation by coral cells in vitro

Helman¹,

Natale²,

Sherrell

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The evolution of multicellularity in animals required the production of extracellular matrices that serve to spatially organize cells according to function. In corals, three matrices are involved in spatial organization: (i) an organic ECM, which facilitates cell-cell and cell-substrate adhesion; (ii) a skeletal organic matrix (SOM), which facilitates controlled deposition of a calcium carbonate skeleton; and (iii) the calcium carbonate skeleton itself, which provides the structural support for the 3D organization of coral colonies. In this report, we examine the production of these three matrices by using an in vitro culturing system for coral cells. In this system, which significantly facilitates studies of coral cell physiology, we demonstrate in vitro excretion of ECM by primary (nondividing) tissue cultures of both soft (Xenia elongata) and hard (Montipora digitata) corals. There are structural differences between the ECM produced by X. elongata cell cultures and that of M. digitata, and ascorbic acid, a critical cofactor for proline hydroxylation, significantly increased the production of collagen in the ECM of the latter species. We further demonstrate in vitro production of SOM and extracellular mineralized particles in cell cultures of M. digitata. Inductively coupled plasma mass spectrometry analysis of Sr/Ca ratios revealed the particles to be aragonite. De novo calcification was confirmed by following the incorporation of 45 Ca into acid labile macromolecules. Our results demonstrate the ability of isolated, differentiated coral cells to undergo fundamental processes required for multicellular organization.aragonite ͉ cell culture ͉ cnidaria ͉ calcification ͉ C orals (class, Anthozoa) are the most basal cnidarians and the first animal phylum with an organized neural system and complex active behavior (1). The embryonic gastrula develops to form an outer ectoderm and an inner endoderm separated by the mesoglea, a noncellular fibrous jelly-like material (2). The two germ layers are spatially structured by an ECM in which embedded, interstitial (stem) cells give rise to nematocysts, mucous glands, and sensory or nerve cells (2, 3). Many corals also precipitate calcium carbonate in the form of aragonite on a skeletal organic matrix (SOM) template (4, 5). The precipitation pattern is highly controlled between colonies, giving rise to morphological structures that are used as primary phenotypic markers of species in extant reefs and fossil samples.The basic cellular processes responsible for the production of ECM, SOM, and calcium carbonate skeleton remain largely unknown. Molecular, genetic, and physiological analyses of cellular processes in corals have been elusive mainly because it is difficult to grow corals under controlled conditions in the laboratory and because of the genetic and physiological complexities inherent in associations of the animals with symbionts and parasites. All zooxanthellate corals harbor intracellular symbiotic dinoflagellates (zooxanthellae) within their endoderm cells; the al...

show abstract

Reduced negative surface charge on arterial endothelium of diabetic rats

Cited by 27 publications

References 21 publications

Alterations of lung structure in experimental diabetes, and diabetes associated with hyperlipidaemia in hamsters

Alterations of lung structure in experimental diabetes, and diabetes associated with hyperlipidaemia in hamsters

A comparison of the insulin-sensitive transport of chromium in healthy and model diabetic rats

Extracellular matrix production and calcium carbonate precipitation by coral cells in vitro

Contact Info

Product

Resources

About