Reduced square Bacillus thuringiensis insecticidal protein content of transgenic cotton under N deficit

Chen, Yuan; Liu, Zhenyu; Tambel, Leila I.M.; Zhang, Xiang; Chen, Dehua

doi:10.1016/s2095-3119(20)63190-2

Cited by 10 publications

(2 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While new proteins are synthesized, there is also degradation of old proteins, and the amino acids produced from degradation can be utilized for protein synthesis [36]. Multiple studies have shown that the concentration of Cry1Ac endotoxin protein content is closely related to protein turnover in plants [37][38][39]. Chen et al [40] proved the altered square nitrogen metabolic intensity was main physiological cause of changed square growth and Cry1Ac endotoxin content.…”

Section: Discussionmentioning

confidence: 99%

Low temperature exposure decreases Cry1Ac insecticidal endotoxin content in cotton seeds

Chen,

Dong,

Dai

et al. 2024

Preprint

View full text Add to dashboard Cite

Background Sudden temperature drops, resulting from extreme weather events, often occur during the boll-setting period of cotton in Xinjiang, China, causing decreased expression of Bacillus thuringiensis (Bt) insecticidal proteins in cotton bolls. The precise threshold temperatures and durations that lead to significant changes in Cry1Ac endotoxin levels under low temperatures remain unclear. To address this, we investigated the effects of different temperatures and stress durations on Cry1Ac endotoxin levels in cotton bolls. In 2020–2021, two Bt transgenic cotton cultivars, conventional Sikang1 and hybrid Sikang3, were selected as experimental materials. Various low temperatures (ranging from 16 to 20°C) with different durations (12h, 24h and 48h) were applied during the peak boll-setting period. Results As the temperature decreased, the Cry1Ac endotoxin content in the boll shell, fiber, and seed exhibited a declining trend. Moreover, the temperature causing a significant change in Cry1Ac endotoxin content increased with the prolonged duration of low-temperature stress. Among the components of cotton bolls, seeds were most affected by low-temperature stress, with the threshold temperature for a significant reduction in Cry1Ac endotoxin content ranging from 17°C to 19°C. Correlation analysis indicated that low temperatures led to a decrease in protein synthesis capacity and an increase in degradation ability, resulting in reduced Cry1Ac endotoxin content. Pathway analysis revealed that both amino acid and peptidase had significant negative effects on Cry1Ac endotoxin content. Conclusion In summary, when the daily average temperature was ≤ 19°C, implementing cultural practices to reduce free amino acid content and peptidase activity could serve as effective cold defense strategies for Bt cotton production.

show abstract

Section: Discussionmentioning

confidence: 99%

Low temperature exposure decreases Cry1Ac insecticidal endotoxin content in cotton seeds

Chen,

Dong,

Dai

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Chen et al [14,32,[37][38][39][40][41] have previously reported on the strong link between the expression of insecticidal proteins and nitrogen metabolism in cotton. This study aims to investigate the effect of low-temperature stress on various physiological parameters of the boll shell in Bt cotton.…”

Section: The Influence Of Low Temperature On Nitrogen Metabolism Resu...mentioning

confidence: 99%

Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism

Liu

et al. 2023

Plants

Self Cite

View full text Add to dashboard Cite

Low temperature is the main factor for global natural disasters affecting the growth and distribution of plants, and cotton may be affected by low temperature and cold damage at all growth stages. In addition, the insecticidal resistance of cultivars has been reported to perform poorly or unstably due to adverse environments. The present study aimed to investigate the impact of low temperature on the levels of insecticidal protein in Bacillus thuringiensis (Bt) transgenic cotton plants during the peak boll stage. To achieve this, two Bt cotton cultivars, Sikang1 (SK1) and Sikang3 (SK3), were subjected to different temperature regimes and durations. The findings of the study demonstrated that the expression of insecticidal protein in the boll shell of Bt transgenic cotton plants was significantly inhibited under low-temperature stress. Specifically, in 2020, compared to the CK (27 °C), the insecticidal protein content in the boll shell of SK3 decreased by 28.19% after a 48 h of a 16 °C temperature. These results suggest that low-temperature stress can negatively impact the expression of insecticidal protein in Bt transgenic cotton, highlighting the need for appropriate measures to minimize its adverse effects on cotton production. In addition, the threshold temperature that leads to a significant decrease in the content of insecticidal proteins symbolizes an upward trend as the duration of stress prolongs. Decreased Bt protein content at low temperatures is associated with changes in the N metabolism. The present study revealed a significant positive correlation between the levels of glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) activities, as well as in the soluble protein levels in the boll shell and the content of the Bt protein. On the other hand, a significant negative correlation was observed between the levels of free amino acids, peptidase, and protease activities, as well as of Bt protein content. These findings suggest that, in Bt cotton production, it is crucial to remain vigilant of prolonged low-temperature disasters, which last for over 12 h and drop below 17–20 °C during the peak boll stage. Such conditions may reduce insecticidal resistance, leading to substantial economic losses.

show abstract

Enhancing boll protein synthesis and carbohydrate conversion by the application of exogenous amino acids at the peak flowering stage increased the boll Bt toxin concentration and lint yield in cotton

LIU

LI²,

Tambel³

et al. 2023

Journal of Integrative Agriculture

View full text Add to dashboard Cite

Reduced square Bacillus thuringiensis insecticidal protein content of transgenic cotton under N deficit

Cited by 10 publications

References 23 publications

Low temperature exposure decreases Cry1Ac insecticidal endotoxin content in cotton seeds

Low temperature exposure decreases Cry1Ac insecticidal endotoxin content in cotton seeds

Effect of Low Temperature on Insecticidal Protein Contents of Cotton (Gossypium herbaceum L.) in the Boll Shell and Its Physiological Mechanism

Enhancing boll protein synthesis and carbohydrate conversion by the application of exogenous amino acids at the peak flowering stage increased the boll Bt toxin concentration and lint yield in cotton

Contact Info

Product

Resources

About