Reduced time HPLC analyses for fast quality control of<i>citrus</i>essential oils

Russo, Marina; Bonaccorsi, Ivana; Costa, Rosaria; Trozzi, Alessandra; Dugo, Paola; Mondello, Luigi

doi:10.1080/10412905.2015.1027419

Cited by 32 publications

(43 citation statements)

References 16 publications

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“…Under the optimum condition, the LODs and LOQs of coumarin and its derivatives were in the range of 0.5-1.7 µg·kg −1 and 1.7-5.2 µg·kg −1 , respectively. It is worth mentioning that the LODs of coumarins determined by previous published methods, such as GC-MS [34,35] and HPLC [24,25,36] were around 10-100 µg·kg −1 , which is much higher than the LODs determined by the HPLC-MS/MS method developed in this study (0.5 µg·kg −1 ). The sensitivity of this method was highly improved over conventional GC methods and the lower LOD makes it possible to determine trace amounts of coumarin compounds in real samples.…”

Section: Calibration and Sensitivitycontrasting

confidence: 64%

“…So far, a variety of analytical methods based on GC [20,21], GC-MS [22], HPLC [23][24][25][26][27], and LC-MS [28,29] have been developed to analyze coumarins, but the low accuracy, high organic reagent consumption and long analysis times of these conventional methods are primary obstacles because of the complex matrix effects of coumarins. In recent years, tandem mass spectrometry (MS/MS) has been reported to provide a much higher degree of assurance than the single stage mass spectrometry technique when determining analytes in many complex matrix [30].…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

et al. 2016

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Abstract:In this paper an analytical method based on high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) for the determination of coumarin and its derivatives in tobacco products was developed. The MS/MS fragmentation pathways of the eight coumarins were elucidated. The new analytical method was defined based on two main axes, an extraction procedure with acetonitrile and analyte detection performed by HPLC-MS/MS in electron impact mode. The excellent selectivity and sensitivity achieved in multiple reaction monitoring (MRM) mode allowed satisfactory confirmation and quantitation for the coumarin flavor additives. Under the optimized gradient elution conditions, it took only 4.5 min to separate all eight coumarins. Good linearity for all the analytes were confirmed by the correlation coefficient r 2 , ranging from 0.9987 to 0.9996. The limits of detection (LODs) and limits of quantitation (LOQs) of these compounds were in the range of 0.5-1.7 µg/kg and 1.7-5.2 µg/kg, respectively. The average recoveries at three spiked levels (LOQ, 1.5LOQ, 2LOQ) were all in the range of 69.6%-95.1% with RSDs (n = 6) lower than 5.3%. The method of HPLC-MS/MS developed in this study was initially applied to the research of coumarin flavor additives in tobacco products collected from the located market in Beijing from China and proved to be accurate, sensitive, convenient and practical.

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Section: Calibration and Sensitivitycontrasting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

et al. 2016

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“…The commercial value of essential oils is also subject to sensible shifts as happens for every natural product strictly related to crop production. To meet the market demand, essential oils are often reconstituted, starting from cheaper oils, lowering the price and compromising their quality as well …”

Section: Introductionmentioning

confidence: 99%

Rapid discrimination of bergamot essential oil by paper spray mass spectrometry and chemometric analysis

et al. 2016

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A novel approach for the rapid discrimination of bergamot essential oil from other citrus fruits oils is presented. The method was developed using paper spray mass spectrometry (PS-MS) allowing for a rapid molecular profiling coupled with a statistic tool for a precise and reliable discrimination between the bergamot complex matrix and other similar matrices, commonly used for its reconstitution. Ambient mass spectrometry possesses the ability to record mass spectra of ordinary samples, in their native environment, without sample preparation or pre-separation by creating ions outside the instrument. The present study reports a PS-MS method for the determination of oxygen heterocyclic compounds such as furocoumarins, psoralens and flavonoids present in the non-volatile fraction of citrus fruits essential oils followed by chemometric analysis. The volatile fraction of Bergamot is one of the most known and fashionable natural products, which found applications in flavoring industry as ingredient in beverages and flavored foodstuff. The development of the presented method employed bergamot, sweet orange, orange, cedar, grapefruit and mandarin essential oils. PS-MS measurements were carried out in full scan mode for a total run time of 2 min. The capability of PS-MS profiling to act as marker for the classification of bergamot essential oils was evaluated by using multivariate statistical analysis. Two pattern recognition techniques, linear discriminant analysis and soft independent modeling of class analogy, were applied to MS data. The cross-validation procedure has shown excellent results in terms of the prediction ability because both models have correctly classified all samples for each category. Copyright © 2016 John Wiley & Sons, Ltd.

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“…To comply with regulations and low expected concentration limits, sensitive and specific analytical methods might be used. High‐performance liquid chromatography coupled with a diode array detector (HPLC‐DAD) is the method of choice generally reported for their analysis . In 2004, Frérot et al proposed a reversed phase HPLC method with UV detection at 310 nm to separate and quantify the 15 most common furocoumarins ( 1 – 15 ) in essential oils .…”

Section: Introductionmentioning

confidence: 99%

“…Highperformance liquid chromatography coupled with a diode array detector (HPLC-DAD) is the method of choice generally reported for their analysis. [15][16][17][18][19][20] In 2004, Frérot et al proposed a reversed phase HPLC method with UV detection at 310 nm to separate and quantify the 15 most common furocoumarins (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) in essential oils. 18 This method was validated in a collaborative ring test by the IFRA Analytical Working Group 8 years later.…”

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confidence: 99%

HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

Borgne¹,

Cicchetti²,

Bertrand³

2017

Flavour & Fragrance J

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The concentration of furocoumarins, known to induce mutagenesis, carcinogenesis and photodermatitis under UV radiation, is limited by the EU cosmetic regulation in consumer products such as cosmetics, sun protection and bronzing formulations. The bergapten concentration is also limited by the International Fragrance Association. Analytical methods have been proposed for their quantification using high‐performance liquid chromatography (HPLC) coupled with UV detection or mass spectrometry with limits of detection (LOD) and quantification (LOQ) in accordance with the regulation. These methods are time and solvent consuming for routine quality control analyses. In this context, simple and fast HPLC methods in one‐ (1‐D) and two‐ (2‐D) dimensions were developed for the simultaneous analysis of the 15 most common furocoumarins reported in Citrus essential oils: psoralen, bergapten, xanthotoxin, isopimpinellin, oxypeucedanin, oxypeucedanin hydrate, byakangelicol, byakangelicin, heraclenin, 8‐geranyloxypsoralen, bergamottin, imperatorin, isoimperatorin, phellopterin, epoxybergamottin along with five other furocoumarins known to be present in Angelica, bergamot or Heracleum species: angelicin, bergaptol, isobergapten, psora‐4 and 5‐(4‐phenoxybutoxy)psoralen. The 1‐D method was validated for the quantification of bergapten using a Camag TLC Scanner as a detector. The LOD was determined at 2 mg/kg. The method was linear from 2 to 100 mg/kg and the method range was defined using accuracy profiles at with an 80% tolerance interval, with a 30% error, to be 5–100 mg/kg in acetonitrile and 13–100 mg/kg in matrix.

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Reduced time HPLC analyses for fast quality control ofcitrusessential oils

Cited by 32 publications

References 16 publications

Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

Simultaneous Determination of Coumarin and Its Derivatives in Tobacco Products by Liquid Chromatography-Tandem Mass Spectrometry

Rapid discrimination of bergamot essential oil by paper spray mass spectrometry and chemometric analysis

HPTLC methods for qualitative and quantitative analysis of selected furocoumarins in essential oils

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