Reducing oxidative stress by κ-carrageenan and C60HyFn: The post-thaw quality and antioxidant status of Azari water buffalo bull semen

Ramazani, N.; Gharebagh, Farid Mahd; Soleimanzadeh, Ali; Arslan, Halil Ozancan; KELES, Esin; Gradinarska-Yanakieva, D.G.; Arslan‐Acaröz, Damla; Zhandi, Mahdi; Baran, A.; Ayen, Esmail; Dinç, D.A.

doi:10.1016/j.cryobiol.2023.04.003

Cited by 8 publications

(5 citation statements)

References 69 publications

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“…Sperm motility is dependent on various metabolic processes and regulatory systems. Two important mechanisms that regulate sperm motility are the calcium (Ca2+) pathway and the cyclic adenosine monophosphate (cAMP) pathway-dependent protein kinase or protein kinase A [ 38 , 40 , 75 ]. Testicular injury can disrupt the normal functioning of the calcium signaling pathway, which is essential for sperm motility and fertilization.…”

Section: Discussionmentioning

confidence: 99%

“…This disruption can lead to decreased sperm motility and fertility [ 76 ]. Thus, due to the potential damage caused by HM and HTS in the testis, they may lead to a reduction in sperm motility by interfering with the calcium pathways [ 38 , 40 , 75 , 77 ]. In addition to gene mutations, sperm abnormalities can also lead to changes in sperm motility [ 78 – 80 ].…”

Section: Discussionmentioning

confidence: 99%

“…One volume of semen was mixed with two volumes of 1% eosin, and the mixture was then examined at 400× magnification using a light microscope (model CHT, Olympus Optics Co. Ltd.). Live spermatozoa were white and transparent on the head since they did not absorb color, while the dead spermatozoa absorbed a red-purple color [ 40 – 46 ] Eosin-nigrosine staining was also performed to analyze the percentage of abnormal sperm. Sperm that had parts of their head, neck, or tail discolored were considered dead sperm.…”

Section: Methodsmentioning

confidence: 99%

“…Hypoosmotic Swelling Test (HOST) involves diluting 10 µL of semen in 100 µL of a hypoosmotic solution containing 1.35 g fructose and 0.73 g sodium citrate and incubating for 30 min at 37 °C [ 40 , 49 ]. After incubation, sperm are checked for sperm PMF using a contrast phase microscope (Olympus, BX41, Tokyo, Japan) at 400× magnification.…”

Section: Methodsmentioning

confidence: 99%

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Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Baqerkhani,

Soleimanzadeh,

Mohammadi

2024

BMC Vet Res

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Objectives The aim of the present study was to examine donkey sperm quality after intratesticular injection of hypertonic mannitol (HM) and saline (HS). Methods Randomly assigned to five treatment groups were 15 adult male donkeys: (1) Control group (no treatment), (2) Surgery group (surgical castration for testosterone control), (3) NS group (normal saline intratesticular injection), (4) HS group (hypertonic saline), and (5) HM group. We injected 20 mL per testicle. We took 5 mL blood from all donkeys before injection. Castration was performed under general anesthesia 60 days later. Samples included blood and testicular tissue. Total motility (TM), progressive motility (PM), movementy features, DNA damage, morphology, viability, and plasma membrane functionality were evaluated. Hormone analyses, histomorphometric studies and oxidative stress indices including total antioxidant capacity (TAC), glutathione peroxidase (GPx), glutathione (GSH), superoxide dismutase (SOD), malondialdehyde (MDA), and NADP+/NADPH were evaluated. Apoptosis, pyroptosis-related Bax, Caspase-1, GSDMD, and Bcl-2 expression were also assessed. Results In HS and HM groups, testosterone, epididymal sperm count, motility, viability, and plasma membrane functionality dropped while sperm DNA damage increased. HS and HM groups had significantly lower histomorphometric parameters, TAC, GPx, SOD, GSH, and Bcl-2 gene expression. MDA, NADP+/NADPH, Bax, Caspase-1, and GSDMD gene expression were substantially higher in the HS and HM groups than in the control group. Conclusions Toxic effects of hypertonic saline and mannitol on reproductive parameters were seen following, hence, they might be considered as a good chemical sterilizing treatment in donkeys.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Baqerkhani,

Soleimanzadeh,

Mohammadi

2024

BMC Vet Res

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“…Free TQ has anti-oxidative (increasing superoxide dismutase and reducing glutathione enzymes) and antiapoptotic (decreasing the expression of Bax and Caspase-3 in testicular tissues) properties in rats [ 31 ]. Various medicinal plants added to the freezing extender can improve the in vivo fertility of buffalo semen, such as Moringa olifera leaf extract [ 8 ], κ-carrageenan [ 60 ], and other antioxidants [ 11 ].…”

Section: Discussionmentioning

confidence: 99%

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

Khalil,

Hassan,

El-Harairy

et al. 2023

Animals

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Thymoquinone nanoparticles (TQNPs) are broadly utilized in numerous pharmaceutical applications. In the present study, we tested the effects of TQNP supplementation on sperm quality and kinematics, acrosome exocytosis, oxidative biomarkers, apoptosis-like and morphological changes of frozen–thawed buffalo sperm, as well as the fertilizing capacity. Semen was collected from buffalo bulls, diluted (1:10; semen/extender), and divided into five aliquots comprising various concentrations of TQNP 0 (CON), 12.5 (TQNP12.5), 25 (TQNP25), 37.5 (TQNP37.5), and 50 (TQNP50) µg/mL, and then cryopreserved and stored in liquid nitrogen (−196 °C). The results revealed that TQNPs (25 to 50 µg/mL) provided the most optimal results in terms of membrane integrity (p < 0.001) and progressive motility (p < 0.01). In contrast, TQNP50 resulted in a greater post-thawed sperm viability (p = 0.02) compared with other groups. The addition of TQNPs to the extender had no discernible effects on sperm morphology measures. Sperm kinematic motion was significantly improved in the TQNP50 group compared to the control group (p < 0.01). TQNPs effectively reduced the content of H2O2 and MDA levels and improved the total antioxidant capacity of post-thawed extended semen (p < 0.01). The addition of TQNP significantly increased the number of intact acrosomes (p < 0.0001) and decreased the number of exocytosed acrosomes (p < 0.0001). A significant reduction in apoptosis-like changes was observed in TQNP groups. The non-return rates of buffalo cows inseminated with TQNP50-treated spermatozoa were higher than those in the control group (p < 0.05; 88% vs. 72%). These findings suggested that the freezing extender supplemented with TQNPs could effectively enhance the cryotolerance and fertility of buffalo sperm.

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The influence of rutin and chlorogenic acid on oxidative stress and in vivo fertility: Evaluation of the quality and antioxidant status of post‐thaw semen from Azari water buffalo bulls

Mohammadi,

Hosseinchi Gharehaghaji

2024

Veterinary Medicine & Sci

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BackgroundThe vulnerability of buffalo sperm to cryoinjury necessitates the improvement of sperm cryo‐resistance as a critical strategy for the widespread use of assisted reproductive technologies in buffalo.ObjectivesThe main aim of the present study was to evaluate the effects of different concentrations of rutin and chlorogenic acid (CGA) on buffalo semen quality, antioxidant activity and fertility during cryopreservation.MethodsThe semen was collected and pooled from the 3 buffaloes using an artificial vagina (18 ejaculations). The pooled sperm were divided into nine different groups: control (Tris‐based extender); 0.4, 0.6, 0.8 and 1 mM rutin (rutin + Tris‐based extender); and 50, 100, 150 and 200 µM CGG (CGA + Tris‐based extender). Sperm kinematics, viability, hypo‐osmotic swelling test, mitochondrial activity, antioxidant activities and malondialdehyde (MDA) concentration of frozen and thawed buffalo sperm were evaluated. In addition, 48 buffalo were finally inseminated, and pregnancy was rectally determined 1 month after insemination.ResultsCompared to the control group, adding R‐0.4, R‐0.6, CGA‐100 and CGA‐150 can improve total and progressive motility, motility characteristics, viability, PMF and DNA damage in buffalo sperm. In addition, the results showed that R‐0.4, R‐0.6, CGA‐50, CGA‐100 and CGA‐150 increased total antioxidant capacity, catalase, glutathione peroxidase and glutathione activities and decreased MDA levels compared to the control group. Furthermore, it has been shown that adding 150 µM CGA and 0.6 mM rutin to an extender can increase in vivo fertility compared to the control group.ConclusionsIn conclusion, adding rutin and CGA to the extender improves membrane stability and in vivo fertility of buffalo sperm by reducing oxidative stress.

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Reducing oxidative stress by κ-carrageenan and C60HyFn: The post-thaw quality and antioxidant status of Azari water buffalo bull semen

Cited by 8 publications

References 69 publications

Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Effects of intratesticular injection of hypertonic mannitol and saline on the quality of donkey sperm, indicators of oxidative stress and testicular tissue pathology

Supplementation of Thymoquinone Nanoparticles to Semen Extender Boosts Cryotolerance and Fertilizing Ability of Buffalo Bull Spermatozoa

The influence of rutin and chlorogenic acid on oxidative stress and in vivo fertility: Evaluation of the quality and antioxidant status of post‐thaw semen from Azari water buffalo bulls

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