Reducing the time rabbit sperm are held at 5 °C negatively affects their fertilizing ability after cryopreservation

Mocé, E.; Blanch, E.; Talaván, A.; Viudes-de-Castro, M.P.

doi:10.1016/j.theriogenology.2014.07.023

Cited by 6 publications

(7 citation statements)

References 15 publications

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“…were better than those obtained by other authors (Moc e, Lavara, & Vicente, 2010;Moc e et al, 2014;Viudes-de-Castro et al, 2014) who diluted the semen with freezing extender (containing CPA) at room temperature, that is, without a cooling phase, while results similar to ours were obtained by Moc e, Lavara, and Vicente (2003) and Maeda et al (2012).…”

Section: Moreover Our In Vitro (Motility and Viability) And In Vivo supporting

confidence: 77%

“…Thus, we hypothesize that a 90 min cooling time prepares the sperm more adequately for the addition of the CPAs (DMSO and sucrose). Rabbit semen is not generally cooled before the CPA is added for cryopreservation (Mocé et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…The 90 min cooling time is a considerably long time span to process semen samples before freezing them, thus time reduction of this step could substantially decrease the whole time required for semen processing and allow for more ejaculates to be frozen within a given time. In this regard, also Mocé, Blanch, Talaván, and Viudes de Castro () performed a study on the equilibration time to shorten the semen cryopreservation process for rabbit semen.…”

Section: Introductionmentioning

confidence: 99%

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Initial cooling time before freezing affects post‐thaw quality and reproductive performance of rabbit semen

Iorio

Colonna

Miranda

et al. 2018

Animal Science Journal

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The aim of this study was to investigate the effect of initial cooling time at 5°C during semen cryopreservation on post-thaw quality and reproductive performance of rabbit semen. Pooled semen samples (n = 6) were divided into two subsamples and cooled at 5°C for 45 or 90 min. After cooling, the semen samples were diluted to a ratio of 1:1 (v:v) with a freezing extender composed of Tris-citrate-glucose (TCG) containing 16% of dimethylsulfoxide and 0.1 mol/L sucrose. The semen was subsequently loaded in 0.25 ml straws, equilibrated at 5°C and frozen in liquid nitrogen vapor. After thawing, sperm motility, viability, osmotic resistance, acrosome and DNA integrity were assessed. Our results indicate that the longer cooling time, that is, 90 min before cryopreservation significantly improves sperm post-thaw viability, motility and fertility. In fact, reproductive performances obtained with semen frozen after a 90 min cooling time were similar to those produced by fresh semen insemination. Hence, the present research provides an effective freezing protocol for rabbit semen that will allow for the creation of a sperm cryobank for the conservation of Italian rabbit genetic resources, as well as the use of frozen semen doses in commercial farms.

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Section: Moreover Our In Vitro (Motility and Viability) And In Vivo supporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Initial cooling time before freezing affects post‐thaw quality and reproductive performance of rabbit semen

Iorio

Colonna

Miranda

et al. 2018

Animal Science Journal

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“…Additionally, in rabbits, time held at 5 °C affects the quality and reproductive performance of sperm. It was reported that longer holding time (90 min) at 5 °C increases the quality of frozen–thawed rabbit sperm and their fertilizing ability [ 50 ], and conversely, and shortened holding time (10 min) decreases it [ 51 ].…”

Section: Effects Of Freezing and Thawing Proceduresmentioning

confidence: 99%

Strategies for Highly Efficient Rabbit Sperm Cryopreservation

Nishijima

Kitajima

Matsuhisa

et al. 2021

Animals

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The rabbit is a valuable animal for both the economy and biomedical sciences. Sperm cryopreservation is one of the most efficient ways to preserve rabbit strains because it is easy to collect ejaculate repeatedly from a single male and inseminate artificially into multiple females. During the cooling, freezing and thawing process of sperms, the plasma membrane, cytoplasm and genome structures could be damaged by osmotic stress, cold shock, intracellular ice crystal formation, and excessive production of reactive oxygen species. In this review, we will discuss the progress made during the past years regarding efforts to minimize the cell damage in rabbit sperms, including freezing extender, cryoprotectants, supplements, and procedures.

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“…Fertility when there is use of frozen-thawed semen for AI varies depending on experimental approaches. The effectiveness of rabbit semen cryopreservation and maintenance of spermatozoa viability is dependent on different factors such as cryopreservation protocol, cryoprotective agent (CPA), genetic line of males, number of spermatozoa used for AI and those aspects associated with the individual per se, probably related to the proteome of both the seminal plasma and the sperm membrane (Mocé et al, 2014(Mocé et al, , 2015Viudes de Castro et al, 2014a;Casares-Crespo et al, 2018, 2019. The main CPAs currently used for preservation of rabbit semen are a combination of permeating compounds such as acetamide or dimethyl sulphoxide (Me2SO) and non-permeating compounds, such as lactose, sucrose, raffinose or trehalose.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen–thawed rabbit sperm quality variables and reproductive performance

Viudes-de-Castro

Talaván

Vicente

2021

Animal Reproduction Science

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Reducing the time rabbit sperm are held at 5 °C negatively affects their fertilizing ability after cryopreservation

Cited by 6 publications

References 15 publications

Initial cooling time before freezing affects post‐thaw quality and reproductive performance of rabbit semen

Initial cooling time before freezing affects post‐thaw quality and reproductive performance of rabbit semen

Strategies for Highly Efficient Rabbit Sperm Cryopreservation

Evaluation of dextran for rabbit sperm cryopreservation: Effect on frozen–thawed rabbit sperm quality variables and reproductive performance

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