Reduction in Toxicity for Red Blood Cells in Buffered Solutions Containing High Concentrations of 2,3-Butanediol by Trehalose, Sucrose, Sorbitol, or Mannitol

Boutron, P.; Peyridieu, Jean-François

doi:10.1006/cryo.1994.1044

Cited by 26 publications

(9 citation statements)

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“…The use of 2,3 BD (6% = 0.7M) as a supplemental SIM in this study is well within the toxic limits since we have previously published data showing tolerance of pancreatic islets exposed to up to 3M 2,3 BD [39,41]. Moreover, it is now established that both the concentration needed to vitrify and the toxicity can be reduced by incorporating a wide variety of non-permeating disaccharides or polymeric compounds [2,36]; hence the selection of PEG400, which we have previously reported to elevate the glass transition temperature, reduce the energy associated with the transition, and reduce the risk of devitrification in a concentration dependent manner [4]. 1,3-CHD falls within the sub-classification of SIBs that are specifically designed to lattice-match with the basal plane of an ice crystal by hydrogen bonding and thereby inhibit ice crystal growth [10,40].…”

Section: Methodsmentioning

confidence: 55%

Thermal expansion of the cryoprotectant cocktail DP6 combined with synthetic ice modulators in presence and absence of biological tissues

2012

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This study explores physical effects associated with the application of cryopreservation via vitrification using a class of compounds which are defined here as synthetic ice modulators (SIMs). The general classification of SIMs includes molecules that modulate ice nucleation and growth, or possess properties of stabilizing the amorphous state, by virtue of their chemical structure and at concentrations that are not explained on a purely colligative basis. A subcategory of SIMs, referred to in the literature as synthetic ice blockers (SIBs), are compounds that interact directly with ice nuclei or crystals to modify their structure and/or rate of growth. The current study is part of an ongoing effort to characterize thermo-mechanical effects during vitrification, with emphasis on measuring the physical property of thermal expansion—the driving mechanism to thermo-mechanical stress. Materials under investigation are the cryoprotective agent (CPA) cocktail DP6 in combination with one of the following SIMs: 12% polyethylene glycol 400, 6% 1,3 cyclohexanediol, and 6% 2,3 butanediol. Results are presented for the CPA-SIM cocktail in the absence and presence of bovine muscle and goat artery specimens. This study focuses on the upper part of the cryogenic temperature range, where the CPA behaves as a fluid for all practical applications. Results of this study indicate that the addition of SIMs to DP6 allows lower cooling rates to ensure vitrification and extends the range of measurements. It is demonstrated that the combination of SIM with DP6 increases the thermal expansion of the cocktail, with implications for the likelihood of fracture formation—the most dramatic outcome of thermo-mechanical stress.

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Section: Methodsmentioning

confidence: 55%

Thermal expansion of the cryoprotectant cocktail DP6 combined with synthetic ice modulators in presence and absence of biological tissues

2012

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“… 142 Hemolysis from BD containing 3.1% wt/wt of meso-isomer at 4°C was drastically reduced by adding 4% wt/wt of trehalose, sucrose, sorbitol, or mannitol. 144 Trehalose and sucrose reduced hemolysis more effectively than sorbitol or mannitol.…”

Section: Toxicity Reduced By Combining Cpasmentioning

confidence: 96%

Cryoprotectant Toxicity: Facts, Issues, and Questions

Best

2015

Rejuvenation Research

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High levels of penetrating cryoprotectants (CPAs) can eliminate ice formation during cryopreservation of cells, tissues, and organs to cryogenic temperatures. But CPAs become increasingly toxic as concentration increases. Many strategies have been attempted to overcome the problem of eliminating ice while minimizing toxicity, such as attempting to optimize cooling and warming rates, or attempting to optimize time of adding individual CPAs during cooling. Because strategies currently used are not adequate, CPA toxicity remains the greatest obstacle to cryopreservation. CPA toxicity stands in the way of cryogenic cryopreservation of human organs, a procedure that has the potential to save many lives. This review attempts to describe what is known about CPA toxicity, theories of CPA toxicity, and strategies to reduce CPA toxicity. Critical analysis and suggestions are also included.

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“…Palasz et al [8] reported that the survival of in vivo-derived bovine embryos frozen-thawed in the presence of 0.7 M glycerol was comparable to those frozenthawed in the presence of 1.4 M glycerol, allowing one-step dilution of post-thaw embryos. In their study, a small amount of sucrose, a non-permeating CPA, was added to the freezing solution to prevent the detrimental effects of rapid and excess influx of water on embryos during one-step dilution [6,9]. We found that most mouse 8-cell embryos can develop into blastocysts even in culture medium containing 0.7 M glycerol, but the addition of sucrose to the medium affected the in vitro development of 8-cell embryos [10].…”

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confidence: 99%

Conventional Freezing of In Vitro-Produced and Biopsied Bovine Blastocysts in the Presence of a Low Concentration of Glycerol and Sucrose

Tominaga

Iwaki

Hochi

2007

Journal of Reproduction and Development

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Abstract. The purpose of this study was to develop a practical cryopreservation method for in vitroproduced (IVP) and sex-predetermined bovine blastocysts that will be applicable to direct transfer of the post-thaw embryos. Blastocysts were harvested 7 days after IVF and allocated to either an intact or biopsy group. The cryoprotective solution contained 0.7 M glycerol and 0, 0.05 or 0.1 M sucrose. Slow cooling at a rate of -0.5 C/min was terminated at -25, -30, or -35 C, and rapid cooling in liquid nitrogen was followed. After one-step thawing and dilution, the IVP blastocysts were cultured for 3 days to assess their survival. The post-thaw survival rate of intact blastocysts after termination of slow cooling at -30 C in 0.7 M glycerol plus 0.1 M sucrose (96.2%) was significantly higher than that at -25 C in 0.7 M glycerol alone (44.4%). The post-thaw survival rate of biopsied bovine blastocysts after termination of slow cooling at -25 C in 0.7 M glycerol alone (53.8%) tended to be lower than that at -25 C in 0.7 M glycerol plus 0.05 M sucrose (91.3%) or -30 C in 0.7 M glycerol plus 0.1 M sucrose (92.3%). Thus, addition of a small amount of sucrose to 0.7 M glycerol cryoprotective solution shortened the process of slow cooling for both the intact and biopsied bovine embryos. Judged from the survival levels in vitro after thawing and one-step dilution of embryos (>80%), this is an improved method of cryopreservation for subsequent direct transfer of IVP and biopsied bovine blastocysts.

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Reduction in Toxicity for Red Blood Cells in Buffered Solutions Containing High Concentrations of 2,3-Butanediol by Trehalose, Sucrose, Sorbitol, or Mannitol

Cited by 26 publications

References 0 publications

Thermal expansion of the cryoprotectant cocktail DP6 combined with synthetic ice modulators in presence and absence of biological tissues

Thermal expansion of the cryoprotectant cocktail DP6 combined with synthetic ice modulators in presence and absence of biological tissues

Cryoprotectant Toxicity: Facts, Issues, and Questions

Conventional Freezing of In Vitro-Produced and Biopsied Bovine Blastocysts in the Presence of a Low Concentration of Glycerol and Sucrose

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