Reduction of Oxidative Stress by Seluang Fish (<i>Rasbora spp.</i>) in Brain of Malnourished Rats (<i>Rattus norvegicus</i>)

Abstract

Malnutrition children have lower intelligence due to brain cells damage caused by oxidative stress. Seluang (Rasbora spp.) has the potential te reduce this problem. This study aimed to analyze the correlation between hydrogen peroxide (H 2 O 2) levels with malondialdehyde (MDA) levels in the brain of malnourished rats after administration of seluang.  This study used a posttest only with control group design. 30 white rats (Rattus norvegicus) made into malnutrition and divided into three groups, M: malnutrition group, P1: malnourished rats fed standard diet, P2: malnourished rats fed seluang for 4 weeks. The results showed that malnourished rats had the highest levels of H 2 O 2. ANOVA statistical tests showed a significant differences between H 2 O 2 levels (p = 0.001) and MDA levels (p = 0.001) among malnutrition group with the other groups Pearson correlation test results concluded that there was a correlation H 2 O 2 levels with MDA levels (p = 0.001, r = 0.684) as indicated by decrease of H 2 O 2 levels followed by decrease in MDA levels. This study showed a correlation between H 2 O 2 levels and MDA levels in the of malnourished rat brain fed seluang. Thus seluang can improve the condition of oxidative stress in malnourished rat brain.

The seluang fish (Rasbora spp.) diet to improve neurotoxicity of endosulfan-induced intrauterine pup's brain through of oxidative mechanism

“…The amino acid content of cysteine which has a thiol group in seluang fish can act as an antioxidant. This had been proven by a study from Triawanti et al [13] malnourished rats given seluang fish showed improvement in oxidative stress conditions. The MDA level decreased, and the enzymatic antioxidant activity was improved.…”

“…A study by Yunanto et al proved that white rats fed with seluang fish had better spatial memory and higher PPARg and PPARa expressions compared to rats fed by standard feed [12]. Triawanti et al study had proven that seluang fish can improve the oxidative stress condition of the brain after malnutrition [13].…”

The supplementation of pasak bumi (Eurycoma longifolia Jack.) in undernourished rats to increase spatial memory through antioxidant mechanism

“…Rats are considered undernourished when plasma protein levels <4,7 g/dL [6]. R. norvegicus's normal protein level is 4,7e5,2 g/dL [7].…”

“…After administration of low protein feeding (AIN-76A) for 8 weeks (56 days) post natal it is known that the mean rats protein level was 1.9 g/dL below the normal level which is 4.7 g/dL [7]. The average body weight after 8 weeks of low protein feeding (AIN-76A) were P1 ¼ 118.3 ± 9.83 g; P2 ¼ 74.28 ± 11.33 g; P3 ¼ 76.67 ± 5.16 g, respectively.…”

“…South Kalimantan has a variety of potential food sources to overcome the problem of undernourished. Previous studies have shown that giving of wild fish from South Kalimantan can increase IGF-1, bone growth, Hb levels, protein [6], overcome oxidative stress in the brain [7], and improve memory [8] in mice that were malnourished.…”