Reduction of plasma clot stability by a novel factor XIIIa inhibitor from the Giant Amazon Leech, Haementeria ghilianii

Anticoagulants from Hematophagous

“…It was also demonstrated that plasma clots formed in the presence of tridegin are more sensitive to lyses by hementin (time required for 50% lysis in the presence and absence of hementin was 16 and about 22 h, respectively) [118]. Study of lysis of clots formed from PRP revealed that in the presence of tridegin the effect of fibrinolytic enzymes was the same as in PPP, whereas lysis of platelet-containing clots occurred slower.…”

“…Antistasin features 119 residues of amino acids with the domain I (residues 1-55) being 56% similar to the domain II (residues . Of the nine residues of the C-terminal (111)(112)(113)(114)(115)(116)(117)(118)(119), domain portion four was positively charged [86], and their active site was located on domain I [88][89][90]. The cDNA of antistasin was cloned [89] and the recombinant protein expressed in system of baculovirus vector in insect cells [90].…”

Differences in the inhibition of coagulation factor XIII-A from animal species revealed by Michael Acceptor– and thioimidazol based blockers

“…A comparable effect was described for Tridegin, a leech-derived natural peptidic FXIIIa-inhibitor [31]. Tridegin reduces clot stability and enhances fibrinolysis in vitro [32,33]. Table 3 Summary of k 2nd -values [10 3 M −1 s −1 ] for the inactivation of rFXIII-A species.…”

Sol Sherry Lecture in Thrombosis

“…45 A protein isolated from the giant amazon leech was described to possess similar properties, although its mode of interaction with factor XIIIa is not yet known. [81][82][83] Positive results were obtained with a monoclonal antibody directed against the thrombin cleavage site of factor XIII, which blocked the activation of the zymogen. 84 Another interesting approach utilizes a monoclonal antibody against ␣ 2 PI.…”