Reductive elimination of superoxide: Structure and mechanism of superoxide reductases

Pinto, A.F.; Rodrigues, João V.; Teixeira, Miguel

doi:10.1016/j.bbapap.2009.10.011

Cited by 58 publications

(85 citation statements)

References 65 publications

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“…Superoxide reductase is an oxidoreductase present only in the anaerobic and facultative microorganisms (234). SOD and catalase provide major antioxidant defenses against ROS.…”

Section: A Endogenous Enzymatic Antioxidantsmentioning

confidence: 99%

Oxidative Stress: An Essential Factor in the Pathogenesis of Gastrointestinal Mucosal Diseases

Bhattacharyya

Chattopadhyay

Mitra

et al. 2014

Physiological Reviews

1,885

1,258

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Reactive oxygen species (ROS) are generated as by-products of normal cellular metabolic activities. Superoxide dismutase, glutathione peroxidase, and catalase are the enzymes involved in protecting cells from the damaging effects of ROS. ROS are produced in response to ultraviolet radiation, cigarette smoking, alcohol, nonsteroidal anti-inflammatory drugs, ischemia-reperfusion injury, chronic infections, and inflammatory disorders. Disruption of normal cellular homeostasis by redox signaling may result in cardiovascular, neurodegenerative diseases and cancer. ROS are produced within the gastrointestinal (GI) tract, but their roles in pathophysiology and disease pathogenesis have not been well studied. Despite the protective barrier provided by the mucosa, ingested materials and microbial pathogens can induce oxidative injury and GI inflammatory responses involving the epithelium and immune/inflammatory cells. The pathogenesis of various GI diseases including peptic ulcers, gastrointestinal cancers, and inflammatory bowel disease is in part due to oxidative stress. Unraveling the signaling events initiated at the cellular level by oxidative free radicals as well as the physiological responses to such stress is important to better understand disease pathogenesis and to develop new therapies to manage a variety of conditions for which current therapies are not always sufficient.

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“…Superoxide reductase is an oxidoreductase present only in the anaerobic and facultative microorganisms (234). SOD and catalase provide major antioxidant defenses against ROS.…”

Section: A Endogenous Enzymatic Antioxidantsmentioning

confidence: 99%

Oxidative Stress: An Essential Factor in the Pathogenesis of Gastrointestinal Mucosal Diseases

Bhattacharyya

Chattopadhyay

Mitra

et al. 2014

Physiological Reviews

1,885

1,258

View full text Add to dashboard Cite

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“…Cells express very efficient antioxidant systems which detoxify these species [1,2]. In the case of the superoxide radical, only two classes of enzymes able to detoxify it have been described: the superoxide dismutase (SOD) [3], expressed in almost all cells living in contact with oxygen, and the more recently discovered superoxide reductase (SOR) which has been found in some microaerophilic and anaerobic bacteria [4][5][6][7][8][9]. The presence of antioxidant systems in anaerobic bacteria, in particular those living in close contact with aerobic biotopes, allows them to survive when they are transiently or accidentally exposed to oxygen [10].…”

Section: Introductionmentioning

confidence: 99%

Intermolecular electron transfer in two-iron superoxide reductase: a putative role for the desulforedoxin center as an electron donor to the iron active site

et al. 2011

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“…In the oxidized state, and in most SORs, a glutamate residue near the iron centre is coordinated to the other axial position of the iron, resulting in an octahedral geometry. In the reduced state the glutamate residue no longer coordinates to the iron, allowing O À 2 to bind (Pinto et al, 2010). The active-site-containing domain adopts a seven-stranded -barrel immunoglobin-like fold.…”

Section: Introductionmentioning

confidence: 99%

“…Superoxide reductases (SORs) constitute a new family of enzymes that are able to scavenge superoxide anion through its reduction only (2) (Jenney et al, 1999;Pinto et al, 2010), which distinguishes them from the SODs. Superoxide reductases have so far only been found in anaerobic and microaerobic organisms.…”

Section: Introductionmentioning

confidence: 99%

Cloning, purification, crystallization and X-ray crystallographic analysis ofIgnicoccus hospitalisneelaredoxin

et al. 2010

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Superoxide reductases (SORs) are metalloproteins which constitute the most recently identified oxygen-detoxification system in anaerobic and microaerobic bacteria and archaea. SORs are involved in scavenging superoxide radicals from the cell by catalyzing the reduction of superoxide (O À 2 ) to hydrogen peroxide and are characterized by a catalytic nonhaem iron centre coordinated by four histidine ligands and one cysteine ligand. Ignicoccus hospitalis, a hyperthermophilic crenarchaeon, is known to have a neelaredoxin-type SOR that keeps toxic oxygen species levels under control. Blue crystals of recombinant I. hospitalis oxidized neelaredoxin (14.1 kDa, 124 residues) were obtained. These crystals diffracted to 2.4 Å resolution in-house at room temperature and belonged to the hexagonal space group P6 2 22 or P6 4 22, with unit-cell parameters a = b = 108, c = 64 Å . Cell-content analysis indicated the presence of one monomer in the asymmetric unit.

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Reductive elimination of superoxide: Structure and mechanism of superoxide reductases

Cited by 58 publications

References 65 publications

Oxidative Stress: An Essential Factor in the Pathogenesis of Gastrointestinal Mucosal Diseases

Oxidative Stress: An Essential Factor in the Pathogenesis of Gastrointestinal Mucosal Diseases

Intermolecular electron transfer in two-iron superoxide reductase: a putative role for the desulforedoxin center as an electron donor to the iron active site

Cloning, purification, crystallization and X-ray crystallographic analysis ofIgnicoccus hospitalisneelaredoxin

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