Reevaluation of Two Quantitative Trait Loci for Type II Resistance to Fusarium Head Blight in Wheat Germplasm PI 672538

X, Li; Xiang, Zepan; Chen, Wanquan; Huang, Qianglan; Liu, Taiguo; Qin, Li; Zhong, Shengyi; Zhang, Min; Guo, Jungang; Li, Lei; Luo, Peigao

doi:10.1094/phyto-04-16-0170-r

Cited by 14 publications

(13 citation statements)

References 64 publications

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“…We found that L693 exhibited a high level of FHB resistance [ 20 ]; only the inoculated spikelet had dried out and died at 21 days after inoculation (DAI) with F. graminearum . By contrast, in L661, after the same period of time, almost the entire spike containing the inoculated spikelet had dried out and died ( Figure 1 A).…”

Section: Resultsmentioning

confidence: 99%

“…Further analysis of 12 samples showed that the greatest difference in gene expression following F. graminearum infection occurred between different tissues (spike vs. leaf), while the smallest difference was observed between the resistant and susceptible genotypes ( Figure 3 B). This could be due to the fact that L693 and L661 have similar genetic backgrounds [ 16 , 20 , 23 ]. The fact that the greatest difference in gene expression occurred between the spike and leaf indicated that the transcriptome data were reliable to some degree, which could be a real response after F. graminearum infection.…”

Section: Discussionmentioning

confidence: 99%

“…This segregation in resistance showed that resistance in the F 2 and F 2:3 populations was inherited as two Mendelian factors, FhbL693a and FhbL693b. We then detected two major quantitative trait loci (QTLs), Qfhs-2B and Qfhs-3B , associated with FHB resistance [ 20 ]. Information regarding pedigree, inheritance, resistance response, chromosomal location, and marker diagnosis indicated that Qfhs-3B was different from Fhb1 : it behaved as a single Mendelian factor and was given the gene symbol FhbL693b [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis Identifies a 140 kb Region of Chromosome 3B Containing Genes Specific to Fusarium Head Blight Resistance in Wheat

Zhong

Chen

et al. 2018

IJMS

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Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is one of the most destructive fungal diseases of wheat (Triticum aestivum L.). Because of the quantitative nature of FHB resistance, its mechanism is poorly understood. We conducted a comparative transcriptome analysis to identify genes that are differentially expressed in FHB-resistant and FHB-susceptible wheat lines grown under field conditions for various periods after F. graminearum infection and determined the chromosomal distribution of the differentially expressed genes (DEGs). For each line, the expression in the spike (which exhibits symptoms in the infected plants) was compared with that in the flag leaves (which do not exhibit symptoms in the infected plants). We identified an island of 53 constitutive DEGs in a 140 kb region with high homology to the FhbL693b region on chromosome 3B. Of these genes, 13 were assigned to specific chloroplast-related pathways. Furthermore, one gene encoded inositol monophosphate (IMPa) and two genes encoded ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO). Our findings suggest that the temporary susceptibility in locally infected spikes results from the cross-talk between RuBisCO and IMPa, which blocks secondary signaling pathways mediated by salicylic acid and induces a systemic acquired resistance in the distant leaf tissue.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis Identifies a 140 kb Region of Chromosome 3B Containing Genes Specific to Fusarium Head Blight Resistance in Wheat

Zhong

Chen

et al. 2018

IJMS

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“…Fusarium head blight (FHB), mainly caused by Fusarium graminearum Schwabe and F. culmorum WG Smith, is a serious wheat (Triticum aestivum L.) disease worldwide (Bai & Shaner 2004;Li et al 2017). In China, FHB epidemics have frequently occurred in the middle to lower valleys of the Yangtze River (Yao & Lu 2000).…”

Section: Abstract: Agronomic Trait; Fhb; Resistance Evaluation; Tritimentioning

confidence: 99%

Identification of three new resources of resistance to Fusarium head blight in wheat

Huang¹,

Fatima²,

Zhong³

et al. 2019

CZECH J GENET PLANT

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Fusarium head blight (FHB) mainly caused by Fusarium species is one of the most important diseases threatening worldwide wheat production. To develop new FHB resistance resources, disease resistances of three new wheat lines L958, L962 and L987 were evaluated over a period of several years. We employed L699 (PI672540) and L661 as the resistant and susceptible control, respectively. Moderate FHB resistance was observed in these three wheat lines, among which the genotype L958 was found a good resource for yield, quality and FHB resistance improvement. In addition, genotype L962 was found to be resistant to powdery mildew, and both L958 and L987 carried the YrL693 gene conferring stripe rust resistance. The study provides promising results for accelerating the resistance breeding of the three wheat lines. Keywords: agronomic trait; FHB; resistance evaluation; Triticum aestivumFusarium head blight (FHB), mainly caused by Fusarium graminearum Schwabe and F. culmorum WG Smith, is a serious wheat (Triticum aestivum L.) disease worldwide (Bai & Shaner 2004;Li et al. 2017). In China, FHB epidemics have frequently occurred in the middle to lower valleys of the Yangtze River (Yao & Lu 2000). In recent years, FHB has spread to the southwest of China, including Sichuan Province (Zhang et al. 2011;Liu et al. 2015;Yang et al. 2016), where the climate was warm and rainy. Furthermore, FHB is also a fungal disease threatening food and economic security due to its contamination with mycotoxins, decreasing the wheat yield and quality (Zwart et al. 2008). At present, there is still no economic and effective way to control this disease. Developing resistant cultivars is an important approach to control this disease, for which the decisive step is to identify new germplasm with multi-resistance.Some effective FHB resistance sources have been identified in common wheat (Chu et al. 2011), and some FHB resistance quantitative trait loci (QTLs) have also been found and mapped in wheat relatives (Cai et al. 2005;Chen et al. 2007). In recent years, some geneticists and breeders have paid much ata These authors contributed equally to this work. 16 Original PaperCzech Journal of Genetics and Plant Breeding, 55, 2019 (1) (Luo et al. 2009;Liu et al. 2013). In addition, four new FHB resistant wheat germplasms were identified and released , which demonstrated the possibility of using Th. intermedium to develop novel wheat germplasm with different disease resistance. However, some breeding problems still remain and new materials with effective resistance to various diseases need to be developed.The research in this paper is aimed at screening wheat FHB resistance germplasm derived from an MY11 × YU25 cross for FHB resistance improvement. MATERIAL AND METHODSPlant material. The three wheat lines L958, L962, and L987 were selected from the F 7 populations derived from an MY11 × YU25 cross during 2007. MY11 is a wheat cultivar widely grown in southwestern China, while YU25 is a wheat cultivar with a foreign pedigree of Th. intermedium .FH...

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“…From agronomical traits and application standpoints of view, we developed the following four spring wheat lines: L658 (PI 672537), L693 (PI 672538), L696 (PI 672539), and L699 (PI 672540). These lines exhibited excellent agronomic traits, including yield and resistance to Fusarium head blight conferred by FhbL693a and FhbL693b [ 24 ], to stripe rust conferred by YrL693 [ 25 ] and to powdery mildew conferred by Pm40 [ 22 , 23 , 26 , 27 ]. Though the pedigree of PI 672538 includes Th .…”

Section: Introductionmentioning

confidence: 99%

Collinearity Analysis and High-Density Genetic Mapping of the Wheat Powdery Mildew Resistance Gene Pm40 in PI 672538

Zhong

Fatima

et al. 2016

PLoS ONE

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The wheat powdery mildew resistance gene Pm40, which is located on chromosomal arm 7BS, is effective against nearly all prevalent races of Blumeria graminis f. sp tritici (Bgt) in China and is carried by the common wheat germplasm PI 672538. A set of the F1, F2 and F2:3 populations from the cross of the resistant PI 672538 with the susceptible line L1034 were used to conduct genetic analysis of powdery mildew resistance and construct a high-density linkage map of the Pm40 gene. We constructed a high-density linkage genetic map with a total length of 6.18 cM and average spacing between markers of 0.48 cM.Pm40 is flanked by Xwmc335 and BF291338 at genetic distances of 0.58 cM and 0.26 cM, respectively, in deletion bin C-7BS-1-0.27. Comparative genomic analysis based on EST-STS markers established a high level of collinearity of the Pm40 genomic region with a 1.09-Mbp genomic region on Brachypodium chromosome 3, a 1.16-Mbp genomic region on rice chromosome 8, and a 1.62-Mbp genomic region on sorghum chromosome 7. We further anchored the Pm40 target intervals to the wheat genome sequence. A putative linear index of 85 wheat contigs containing 97 genes on 7BS was constructed. In total, 9 genes could be considered as candidates for the resistances to powdery mildew in the target genomic regions, which encoded proteins that were involved in the plant defense and response to pathogen attack. These results will facilitate the development of new markers for map-based cloning and marker-assisted selection of Pm40 in wheat breeding programs.

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Reevaluation of Two Quantitative Trait Loci for Type II Resistance to Fusarium Head Blight in Wheat Germplasm PI 672538

Cited by 14 publications

References 64 publications

Transcriptome Analysis Identifies a 140 kb Region of Chromosome 3B Containing Genes Specific to Fusarium Head Blight Resistance in Wheat

Transcriptome Analysis Identifies a 140 kb Region of Chromosome 3B Containing Genes Specific to Fusarium Head Blight Resistance in Wheat

Identification of three new resources of resistance to Fusarium head blight in wheat

Collinearity Analysis and High-Density Genetic Mapping of the Wheat Powdery Mildew Resistance Gene Pm40 in PI 672538

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