Reference genes for real-time RT-PCR expression studies in an Antarctic Pseudomonas exposed to different temperature conditions

García-Laviña, César X.; Castro‐Sowinski, Susana; Ramón, Ana

doi:10.1007/s00792-019-01109-4

Cited by 13 publications

(5 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have demonstrated that rpoD is among the most stable RGs in Pseudomonas putida (Chang et al, 2009), Pseudomonas syringae (Smith et al, 2018), and in the psychrotolerant Antarctic isolate Pseudomonas sp. AU10 (García‐Laviña et al, 2019), while other studies have indicated that this gene is not as reliable as internal control and is the least stably expressed in Aeromonas salmonicida subsp. salmonicida (Rivera et al, 2015) and Xanthomonas fragariae (Kałużna et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Selection and validation of reference genes for gene expression studies in Xanthomonas arboricola pv. juglandis subjected to abiotic stress

Petriccione¹,

Magri²,

Gaudiano³

et al. 2021

Plant Pathology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Selection and validation of reference genes for gene expression studies in Xanthomonas arboricola pv. juglandis subjected to abiotic stress

Petriccione¹,

Magri²,

Gaudiano³

et al. 2021

Plant Pathology

View full text Add to dashboard Cite

show abstract

“…For this study we have compared Rds from the hyperthermophile Pyrococcus furiosus ( Pf ) 38 with those from the psychrotolerant organisms Pseudomonas sp. strain AU10 ( Px ) 39 and Polaromonas glacialis ( Pg ). Pf is a marine archaeon with an optimal growth temperature of ~ 100 °C 40 .…”

Section: Introductionmentioning

confidence: 99%

“…Pf is a marine archaeon with an optimal growth temperature of ~ 100 °C 40 . Px is an Antarctic freshwater organism with an optimal growth temperature of 28 °C that can still grow close to 0 °C 39 . Pg has been isolated from alpine glaciers and grows well from 1 to 25 °C 41 , 42 .…”

Section: Introductionmentioning

confidence: 99%

Temperature-dependent iron motion in extremophile rubredoxins – no need for ‘corresponding states’

Jenney,

Wang,

George

et al. 2024

Sci Rep

Self Cite

View full text Add to dashboard Cite

Extremophile organisms are known that can metabolize at temperatures down to − 25 °C (psychrophiles) and up to 122 °C (hyperthermophiles). Understanding viability under extreme conditions is relevant for human health, biotechnological applications, and our search for life elsewhere in the universe. Information about the stability and dynamics of proteins under environmental extremes is an important factor in this regard. Here we compare the dynamics of small Fe-S proteins – rubredoxins – from psychrophilic and hyperthermophilic microorganisms, using three different nuclear techniques as well as molecular dynamics calculations to quantify motion at the Fe site. The theory of ‘corresponding states’ posits that homologous proteins from different extremophiles have comparable flexibilities at the optimum growth temperatures of their respective organisms. Although ‘corresponding states’ would predict greater flexibility for rubredoxins that operate at low temperatures, we find that from 4 to 300 K, the dynamics of the Fe sites in these homologous proteins are essentially equivalent.

show abstract

“…For this study we have compared Rds from the hyperthermophile Pyrococcus furiosus [36] with those from the psychrotolerant organisms Pseudomonas sp. strain AU10 [37] and Polaromonas glacialis. P. furiosus is a marine archaeon with an optimal growth temperature of ~ 100°C [38].…”

Section: Introductionmentioning

confidence: 99%

“…Pseudomonas sp. strain AU10 is an Antarctic freshwater organism with an optimal growth temperature of 28°C that can still grow close to 0°C [37]. P. glacialis has been isolated from alpine glaciers and grows well from 1-25°C [39] [40].…”

Section: Introductionmentioning

confidence: 99%

Temperature-Dependent Iron Motion in Extremophile Rubredoxins – No Need for ‘Corresponding States’

Cramer,

Jenney,

Wang

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Extremophile organisms are known that can metabolize at temperatures down to -25°C (psychrophiles) and up to 122°C (hyperthermophiles). Understanding viability under extreme conditions is relevant for human health, biotechnological applications, and our search for life elsewhere in the universe. Information about the stability and dynamics of proteins under environmental extremes is an important factor in this regard. Here we compare the dynamics of small Fe-S proteins – rubredoxins – from psychrophilic and hyperthermophilic microorganisms, using three different nuclear techniques as well as molecular dynamics calculations to quantify motion at the Fe site. The theory of ‘corresponding states’ posits that homologous proteins from different extremophiles have comparable flexibilities at the optimum growth temperatures of their respective organisms. Although ‘corresponding states’ would predict greater flexibility for rubredoxins that operate at low temperatures, we find that from 4K to 300K, the dynamics of the Fe sites in these homologous proteins are essentially equivalent.

show abstract

Reference genes for real-time RT-PCR expression studies in an Antarctic Pseudomonas exposed to different temperature conditions

Cited by 13 publications

References 53 publications

Selection and validation of reference genes for gene expression studies in Xanthomonas arboricola pv. juglandis subjected to abiotic stress

Selection and validation of reference genes for gene expression studies in Xanthomonas arboricola pv. juglandis subjected to abiotic stress

Temperature-dependent iron motion in extremophile rubredoxins – no need for ‘corresponding states’

Temperature-Dependent Iron Motion in Extremophile Rubredoxins – No Need for ‘Corresponding States’

Contact Info

Product

Resources

About