Reference intervals for LC-MS /MS measurements of plasma renin activity, aldosterone, angiotensin II, and 24-hour urinary aldosterone in Northern Chinese Han population

Yin, Yanzhen; Ma, Chaochao; Yu, Songlin; Ai, Qing; Han, Chuntao; Zhong, Jianxin; Luo, W.; Ma, Xiang; Yu, Jialei; Zhang, Yuanyuan; Cui, Jingwen; Qiu, Ling

doi:10.1016/j.cca.2023.117325

Cited by 5 publications

(2 citation statements)

References 20 publications

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“…Radioimmunoassay showed a significant positive bias on UALD results compared to LC-MS/MS (Lin et al, 2016). For example, the UALD RI derived from 302 healthy subjects by Yin et al based on the LC-MS/MS method was significantly lower than that in other studies (Yin et al, 2023).…”

Section: Discussionmentioning

confidence: 89%

Simultaneous quantitation of urine aldosterone and tetrahydroaldosterone in healthy Chinese subjects using a validated LC–MS/MS method

Cheng,

Lo,

Chan

et al. 2023

Biomedical Chromatography

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Aldosterone (ALD) is excreted in urine mainly as glucuronide conjugates of ALD and tetrahydroaldosterone. Measuring these urinary metabolites might be an alternative screening test to plasma ALD for primary aldosteronism. We report a validated LC–MS/MS method to measure both analytes simultaneously. Urine samples underwent enzymatic hydrolysis to release the analytes from their glucuronide conjugates followed by organic solvent extraction and LC–MS/MS. The analytical performance of this method was evaluated. The within‐batch and between‐batch coefficients of variation for urine ALD and urine THA were all ≤5.2 and ≤3.7%. The lower limit of quantification was 0.5 nmol/L, and the linearity was up to at least 2770 nmol/L for both analytes. No significant matrix interference and carryover were observed. Both analytes in urine were stable for at least 48 h at 10°C and at least 18 months at −80°C. Local reference intervals were established from 126 healthy normotensive Chinese subjects (53% women, age: 20–65 years). Reference intervals for urine ALD and tetrahydroaldosterone were 2–38 and 9–139 nmol/day, respectively. This validated method can be applied to screening and diagnosing primary aldosteronism.

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Section: Discussionmentioning

confidence: 89%

Simultaneous quantitation of urine aldosterone and tetrahydroaldosterone in healthy Chinese subjects using a validated LC–MS/MS method

Cheng,

Lo,

Chan

et al. 2023

Biomedical Chromatography

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show abstract

“…The establishment of LC-MS/MS measurement as the gold standard lead to the reassessment of plasma and urinary aldosterone levels in the clinical practice [ 21 , 22 ]. Accumulating evidence has divulged that most of the commercial assays demonstrate higher aldosterone values compared to the actual values measured using LC-MS/MS [ 14 , 15 , 23 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Investigating the cut-off values of captopril challenge test for primary aldosteronism using the novel chemiluminescent enzyme immunoassay method: a retrospective cohort study

Tezuka,

Omata,

Ono

et al. 2024

Hypertens Res

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The measurement evolution enabled more accurate evaluation of aldosterone production in hypertensive patients. However, the cut-off values for novel assays have been not sufficiently validated. The present study was undertaken to validate the novel chemiluminescent enzyme immunoassay for aldosterone in conjunction with other methods. Moreover, we also aimed to establish a new cut-off value for primary aldosteronism in the captopril challenge test using the novel assay. First, we collected 390 plasma samples, in which aldosterone levels measured using liquid chromatography-mass spectrometry ranged between 0.18 and 1346 ng/dL. The novel chemiluminescent enzyme immunoassay showed identical correlation of plasma aldosterone with liquid chromatography-mass spectrometry, in contrast to conventional radioimmunoassay. Further, we enrolled 299 and 39 patients with primary aldosteronism and essential hypertension, respectively. Plasma aldosterone concentrations measured using the novel assay were lower than those measured by radioimmunoassay, which resulted in decreased aldosterone-to-renin ratios. Subsequently, positive results of the captopril challenge test based on radioimmunoassay turned into “negative” based on the novel assay in 45% patients with primary aldosteronism, using the conventional cut-off value (aldosterone-to-renin activity ratio > 20 ng/dL per ng/mL/h). Receiver operating characteristic curve analysis demonstrated that aldosterone-to-renin activity ratios > 8.2 ng/dL per ng/mL/h in the novel assay was compatible with the conventional diagnosis (sensitivity, 0.874; specificity, 0.980). Our study indicates the great measurement accuracy of the novel chemiluminescent enzyme immunoassay for aldosterone, and the importance of measurement-adjusted cut-offs in the diagnosis of primary aldosteronism.

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Effects of sodium intake, age, gender, blood sampling time on distribution of plasma aldosterone, renin activity, deoxycorticosterone, cortisol, cortisone, and 24 h urinary aldosterone levels in normotensive individuals based on LC-MS/MS

Zhang,

Zhao,

Cheng

et al. 2024

Endocrine

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Reference intervals for LC-MS /MS measurements of plasma renin activity, aldosterone, angiotensin II, and 24-hour urinary aldosterone in Northern Chinese Han population

Cited by 5 publications

References 20 publications

Simultaneous quantitation of urine aldosterone and tetrahydroaldosterone in healthy Chinese subjects using a validated LC–MS/MS method

Simultaneous quantitation of urine aldosterone and tetrahydroaldosterone in healthy Chinese subjects using a validated LC–MS/MS method

Investigating the cut-off values of captopril challenge test for primary aldosteronism using the novel chemiluminescent enzyme immunoassay method: a retrospective cohort study

Effects of sodium intake, age, gender, blood sampling time on distribution of plasma aldosterone, renin activity, deoxycorticosterone, cortisol, cortisone, and 24 h urinary aldosterone levels in normotensive individuals based on LC-MS/MS

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