Reference intervals for serum 24,25-dihydroxyvitamin D and the ratio with 25-hydroxyvitamin D established using a newly developed LC–MS/MS method

Tang, Jonathan; Nicholls, Henry; Piec, Isabelle; Washbourne, Christopher; Dutton, John; Jackson, Sarah; Greeves, Julie P.; Fraser, William D.

doi:10.1016/j.jnutbio.2017.04.005

Cited by 94 publications

(80 citation statements)

References 38 publications

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“…Interassay coefficients of variation were <10% for both 25OHD 2 and 25OHD 3 , and the sum of these analytes is reported here. The 25OHD metabolite, 24,25‐dihydroxyvitamin D, was also measured by tandem mass spectrometry using a prior delipidation procedure to maximize recovery . Overnight fasted blood samples collected at each visit were stored at –80°C, and each participant's complete set was batched together before analysis.…”

Section: Methodsmentioning

confidence: 99%

25-Hydroxyvitamin D Threshold for the Effects of Vitamin D Supplements on Bone Density: Secondary Analysis of a Randomized Controlled Trial

Macdonald

Reid

Gamble

et al. 2018

Journal of Bone and Mineral Research

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Most trials of vitamin D supplementation have shown no benefits on bone mineral density (BMD), although severe vitamin D deficiency causes osteomalacia, which is associated with profound BMD deficits. Recently, the ViDA-BMD study from New Zealand demonstrated a threshold of baseline 25-hydroxyvitamin D (25OHD; 30 nmol/L) below which vitamin D supplementation did benefit BMD. We have now reexamined data from a similar trial in Aberdeen to determine whether a baseline 25OHD threshold of 30 nmol/L is also observed in that database. The Aberdeen study recruited 305 postmenopausal women in late winter and randomized them to receive placebo, vitamin D 400 IU/d, or vitamin D 1000 IU/d over 1 year. As previously reported, BMD loss at the hip was reduced by vitamin D 1000 IU/d only, and there was no significant treatment effect of either dose at the lumbar spine. In the present analysis, when the trial participants were grouped according to whether their baseline 25OHD was ≤30 nmol/L or above this threshold, significant treatment effects were apparent at both the spine and hip in those with baseline 25OHD ≤30 nmol/L, but no significant effects were apparent in those with baseline 25OHD above this level. There was evidence of a similar threshold for effects on parathyroid hormone, but no groups showed changes in bone turnover markers during the study. It is concluded that vitamin D supplements only increase bone density in adults with nadir 25OHD ≤30 nmol/L. This moves us further toward a trial-based definition of vitamin D deficiency in adults with adequate calcium intakes and suggests that supplement use should be targeted accordingly. Future trials of vitamin D supplementation should focus on individuals with 25OHD concentrations in this range. © 2018 American Society for Bone and Mineral Research.

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Section: Methodsmentioning

confidence: 99%

25-Hydroxyvitamin D Threshold for the Effects of Vitamin D Supplements on Bone Density: Secondary Analysis of a Randomized Controlled Trial

Macdonald

Reid

Gamble

et al. 2018

Journal of Bone and Mineral Research

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“…The 25(OH)D is one metabolite of the vitamin D pathway. Tang et al () have reported that measurement of serum 24,25(OH) 2 D can be used as a predictor of vitamin D status. Therefore, the reduced levels of 24,25(OH) 2 D in the skeletal muscle of DHEA mice may imply the decreased vitamin D levels in these animals.…”

Section: Discussionmentioning

confidence: 99%

Nontargeted metabolomic analysis of skeletal muscle in a dehydroepiandrosterone‐induced mouse model of polycystic ovary syndrome

Shen

et al. 2019

Molecular Reproduction Devel

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Polycystic ovary syndrome (PCOS) is the most common endocrinopathy and an important metabolic disorder in women of reproductive age. Insulin resistance (IR) is one of its most important clinical features in patients with PCOS. Androgen excess‐induced mitochondrial dysfunction contributes to skeletal muscle IR in dehydroepiandrosterone (DHEA)‐induced PCOS mice. The effect of androgen excess on the skeletal muscle, however, is incompletely characterized. A nontargeted metabolomics approach was thus applied to analyze the metabolites in skeletal muscle of DHEA‐induced PCOS mice. Data from metabolomic analysis revealed the significant changes in 32 metabolites and the marked impact of five metabolic pathways. ATP production was also found to be significantly reduced in skeletal muscle of DHEA mice. Combined with the quantification of type I and II myofibers and lipid measurement in the skeletal muscle of the mice, the results from the present study supported the role of mitochondrial impairment rather than lipid accumulation in the pathogenesis of skeletal muscle IR in DHEA‐induced PCOS mice. In summary, we show here for the first time the profile of the metabolites in the skeletal muscle of DHEA‐induced PCOS mice which exhibit IR. The work would help better understand the pathology of skeletal muscle IR in PCOS.

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“…Although 1,25(OH) 2 D 3 is primarily considered a regulator of calcium metabolism, almost all nucleated cells have vitamin D receptors (Mellanby, 2016) and receptor activation regulates gene transcription and affects cell proliferation, cell differentiation, and formation of proteins that impact cell phenotype (Garg, Rosella, Lubel, & Gibson, 2013; Teixeira et al, 2012; Zendehdel & Arefi, 2019). Thus, 1,25(OH) 2 D 3 affects a wide range of physiological processes including immunological, cardiovascular, and neoplastic diseases (Fox et al, 2019; Jones, 2008; Mellanby, 2016; Tang et al, 2017). Marmosets have higher serum levels of 1,25(OH) 2 D 3 than rhesus and humans (Shinki et al, 1983; Takahashi et al, 1985; Ziegler et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Serum levels of 25(OH)D 3 in marmosets have been measured with radioimmunoassay (RIA) and liquid chromatography–tandem mass spectrometry (LC–MS; Bosseler et al, 2018; Ziegler et al, 2015). LC–MS is able to differentiate vitamin D 2 from D 3 and to measure all vitamin D metabolites (Tang et al, 2017; Ziegler et al, 2015). There have been limited evaluations of 1,25(OH) 2 D 3 in marmosets; most studies report 25(OH)D 3 (Shinki et al, 1983; Takahashi et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of vitamin D₃ metabolites in Callithrix jacchus (common marmoset)

Goodroe

Fitz²,

Power

et al. 2020

American J Primatol

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Vitamin D 3 (cholecalciferol) is endogenously produced in the skin of primates when exposed to the appropriate wavelengths of ultraviolet light (UV-B). Common marmosets (Callithrix jacchus) maintained indoors require dietary provision of vitamin D 3 due to lack of sunlight exposure. The minimum dietary vitamin D 3 requirement and the maximum amount of vitamin D 3 that can be metabolized by marmosets is unknown. Observations of metabolic bone disease and gastrointestinal malabsorption have led to wide variation in dietary vitamin D 3 provision amongst research institutions, with resulting variation in circulating 25-hydroxyvitamin D 3 (25(OH)D 3 ), the accepted marker for vitamin D sufficiency/deficiency. Multiple studies have reported serum 25(OH)D 3 in captive marmosets, but 25(OH)D 3 is not the final product of vitamin D 3 metabolism. In addition to serum 25(OH)D 3, we measured the most physiologically active metabolite, 1,25-dihydroxyvitamin D 3 (1,25(OH) 2 D 3 ), and the less well understood metabolite, 24,25-dihydroxyvitamin D 3 (24,25(OH) 2 D 3 ) to characterize the marmoset's ability to metabolize dietary vitamin D 3 . We present vitamin D 3 metabolite and related serum chemistry value colony reference ranges in marmosets provided diets with 26,367 (Colony A, N = 113) or 8,888 (Colony B, N = 52) international units (IU) of dietary vitamin D 3 per kilogram of dry matter. Colony A marmosets had higher serum 25(OH)D 3 (426 ng/ml [SD 200] vs. 215 ng/ml [SD 113]) and 24,25(OH) 2 D 3 (53 ng/ml [SD 35] vs. 7 ng/ml [SD 5]). There was no difference in serum 1,25(OH) 2 D 3 between the colonies. Serum 1,25(OH) 2 D 3 increased and 25(OH)D 3 decreased with age, but the effect was weak. Marmosets tightly regulate metabolism of dietary vitamin D 3 into the active metabolite 1,25(OH) 2 D 3 ; excess 25(OH)D 3 is metabolized into 24,25(OH) 2 D 3 . This ability explains the tolerance of high levels of dietary vitamin D 3 by marmosets, however, ourdata suggest that these high dietary levels are not required.

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Reference intervals for serum 24,25-dihydroxyvitamin D and the ratio with 25-hydroxyvitamin D established using a newly developed LC–MS/MS method

Cited by 94 publications

References 38 publications

25-Hydroxyvitamin D Threshold for the Effects of Vitamin D Supplements on Bone Density: Secondary Analysis of a Randomized Controlled Trial

25-Hydroxyvitamin D Threshold for the Effects of Vitamin D Supplements on Bone Density: Secondary Analysis of a Randomized Controlled Trial

Nontargeted metabolomic analysis of skeletal muscle in a dehydroepiandrosterone‐induced mouse model of polycystic ovary syndrome

Evaluation of vitamin D₃ metabolites in Callithrix jacchus (common marmoset)

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Reference intervals for serum 24,25-dihydroxyvitamin D and the ratio with 25-hydroxyvitamin D established using a newly developed LC–MS/MS method

Cited by 94 publications

References 38 publications

25-Hydroxyvitamin D Threshold for the Effects of Vitamin D Supplements on Bone Density: Secondary Analysis of a Randomized Controlled Trial

25-Hydroxyvitamin D Threshold for the Effects of Vitamin D Supplements on Bone Density: Secondary Analysis of a Randomized Controlled Trial

Nontargeted metabolomic analysis of skeletal muscle in a dehydroepiandrosterone‐induced mouse model of polycystic ovary syndrome

Evaluation of vitamin D3 metabolites in Callithrix jacchus (common marmoset)

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Evaluation of vitamin D₃ metabolites in Callithrix jacchus (common marmoset)