Reference Intervals of Reticulated Platelets and Other Platelet Parameters and Their Associations

Abstract

Context.-Reticulated platelets are platelets recently released from the bone marrow, and they can serve as a noninvasive indicator of recent megakaryopoietic activity. Widespread clinical use has been hampered by laborious methods and lack of standardization. Recently, a fully automated method was released on the Abbott CELL-DYN Sapphire hematology analyzer.Objective.-To establish reference ranges for reticulated platelets. Secondary aims were to investigate associations between reticulated platelets and other platelet parameters like mean platelet volume, plateletcrit, and platelet distribution width.Design.-Reticulated platelets and other platelet parameters were measured in an unselected cohort of 8089 subjects visiting a primary health care laboratory. The reticulated platelet data were analyzed using the Bhattacharya technique. In addition, a nonparametric method was used in selected subjects with normal platelet counts for providing reference ranges.Results.-Reticulated platelets ranged from 0.4% to 6.0% or from 1 to 18 3 10 3 /L. Reticulated platelets increased significantly with the subjects' age. Statistically, males had slightly higher values than females, but the differences were negligible. Reticulated platelets were positively correlated with platelet count and negatively with mean platelet volume.Conclusions.-Reference ranges have been established for reticulated platelets as measured on the CELL-DYN Sapphire hematology analyzer. There were no relevant differences between the sexes, but there was a clear effect of age. An individual's reticulated platelets are associated with the platelet count as well as mean platelet volume.

Reticulated platelets: analytical aspects and clinical utility

“…It is true that platelets produced in response to stress megakaryopoiesis are on the average larger, but there is no evidence that this can be extrapolated to normal megakaryopoiesis. In healthy individuals with steady-state platelet production, we found a significant negative association between retPLT and MPV [62]. This is more in keeping with the concept of a constant circulating platelet mass, which would require more small platelets or fewer larger platelets [1].…”

“…However, in a large group of subjects with normal platelet counts, we found a significant negative correlation between retPLT and MPV [62].…”

“…This approach enables correcting for the sizedependent background fluorescence of platelets [39]. In healthy individuals, mean retPLT are between 1.4% and 2.2%, and reported reference ranges are 0.4%-2.8% [59], 0.4% to 4.45%-6.0% [60][61][62] and 1.0%-3.8% [63].…”

“…Stability data on retPLT as measured with CELL-DYN Sapphire range between < 6 h [63] and at least 26 h [62].…”

Neutrophil to Lymphocyte Ratio and Mean Platelet Volume as Inflammatory Indicators in Systemic Lupus Erythematosus Nephritis

“…However, a study with 8,082 individuals from general population has shown that MPV does not correlate with age. 36 In our study, NLR levels were higher in the nephritic group which was also younger than the non-nephritic group. On the other hand, Li et al determined a positive correlation between NLR and age for 3,262 individuals from a healthy population.…”