Reference Materials for Improving Reliability of Multiomics Profiling

Abstract

High-throughput technologies for multiomics or molecular phenomics profiling have been extensively adopted in biomedical research and clinical applications, offering a more comprehensive understanding of biological processes and diseases. Omics reference materials play a pivotal role in ensuring the accuracy, reliability, and comparability of laboratory measurements and analyses. However, the current application of omics reference materials has revealed several issues, including inappropriate selection and underutilization, leading to inconsistencies across laboratories. This review aims to address these concerns by emphasizing the importance of well-characterized reference materials at each level of omics, encompassing (epi-)genomics, transcriptomics, proteomics, and metabolomics. By summarizing their characteristics, advantages, and limitations along with appropriate performance metrics pertinent to study purposes, we provide an overview of how omics reference materials can enhance data quality and data integration, thus fostering robust scientific investigations with omics technologies.

Chemoproteomic profiling by a bioorthogonal probe reveals cell growth inhibition by histone H4 dopaminylation

“…However, we have not yet identified all the modification sites of the enriched proteins or peptides by the pDA probe, primarily due to the length and abundance of the modified peptides as well as the different ionization of pDA from dopamine. Given the crucial role of directly identifying the modification sites by mass spectrometry for later studying their functions, it will be of great interest to further optimize the experimental condition 43 , such as overexpressing the protein of interest to improve the modification level or developing the site-specific antibody.…”