Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability

Hosomi, Silvério Takao; Souza, Thatiane Bonome de; Custódio, Ceci Castilho; Neto, Nelson Barbosa Machado

doi:10.21475/ajcs.17.11.10.pne606

Cited by 15 publications

(18 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An essential factor for the formation of a native orchid seed bank is the identification of the viability of stored seeds which can be used for propagation and conservation of the species, since this viability may be affected even under favorable conditions (Doria 2010, Hosomi et al 2017, Mercado et al 2020. Therefore, the use of fast tests such as tetrazolium is important, mainly to decision-making regarding the management of seed banks (Marcos Filho 2015).…”

Section: Special Supplement: Cerrado [Brazilian Savanna]mentioning

confidence: 99%

“…Methodologies for the tetrazolium test in Orchidaceae seeds have been proposed in the literature (Hosomi et al 2011, Hosomi et al 2012, Hosomi et al 2017, Macedo et al 2014. However, changes in the seed pre-conditioning and conditioning procedures for the test may increase the seed efficiency, since these changes aim to improve the visualization of viable seeds (Salazar & Gélvez 2015, Custódio et al 2016, Seaton et al 2018, Mercado et al 2020.…”

Section: Special Supplement: Cerrado [Brazilian Savanna]mentioning

confidence: 99%

“…f., soaking in distilled water for 24 h provides better results for visualizing the seed viability (Lallana & Garcia 2013). Hosomi et al (2017), evaluating the tetrazolium test on Cattleya labiata and C. tigrina seeds, report that pre-conditioning in a 10 % glucose or 10 % sucrose solution is more effective for visualizing viable seeds. Mercado et al (2020) found that the use of deionized water improves the efficiency of the tetrazolium test on Epidendrum fimbriatum, E. microtum and E. elongatum seeds.…”

Section: Special Supplement: Cerrado [Brazilian Savanna]mentioning

confidence: 99%

See 2 more Smart Citations

Seed viability test of orchids native to the Brazilian Savanna1

Soares

Sorgato

Ribeiro

et al. 2021

Pesqui. Agropecu. Trop.

View full text Add to dashboard Cite

An essential factor for the formation of a native orchid seed bank is the identification of the viability of stored seeds. This study aimed to determine a methodology for optimizing the tetrazolium test, in the identification of the viability of stored seeds of two Orchidaceae medicinal species native to the Brazilian Savanna. Seeds of Miltonia flavescens Lindl. and Schomburgkia crispa Lindl. were submitted to three pre-conditioning conditions: no soaking (control), sucrose solution or distilled water. The seeds were then submitted to a tetrazolium solution, with three types of conditioning: oven, water bath or ambient temperature. The M. flavescens seeds showed a germination of 86.91 %, with a higher percentage of viable seeds (62.32 %) when submitted to pre-conditioning with sucrose + oven conditioning. For S. crispa, the germination was 97.78 %, with a higher percentage of viable seeds for the control treatment + ambient temperature (89.49 %). These results suggest that specific protocols should be used to conduct the tetrazolium test in Orchidaceae. Moreover, when performed on a sample basis, the tetrazolium test should only be used to indicate the seed viability.

show abstract

Section: Special Supplement: Cerrado [Brazilian Savanna]mentioning

confidence: 99%

Section: Special Supplement: Cerrado [Brazilian Savanna]mentioning

confidence: 99%

Section: Special Supplement: Cerrado [Brazilian Savanna]mentioning

confidence: 99%

See 1 more Smart Citation

Seed viability test of orchids native to the Brazilian Savanna1

Soares

Sorgato

Ribeiro

et al. 2021

Pesqui. Agropecu. Trop.

View full text Add to dashboard Cite

show abstract

“…According to methodology adapted from Hosomi, Souza, Custódio and Machado (2017), forty-eight 0.001 g samples of stored seeds of each species were placed in test tubes. Sets of twelve 10 mL tubes were subjected to preconditioning (P) with three soaking pretreatments: P1 -no soaking (control); P2 -3 mL of sucrose solution (10%) for 24 hours; and P3 -3 mL of distilled water for 24 hours.…”

Section: Introductionmentioning

confidence: 99%

“…For C. walkeriana, the highest %VS value was observed when seeds were preconditioned in sucrose and subsequently conditioned in ambient temperature (88%), not differing statistically between preconditioning control and distilled water with subsequent conditioning in ambient temperature (67 and 69%, respectively) and neither of the preconditioning or sucrose in the conditioning water bath (68%) (Table 2). Hosomi et al (2017) at ambient temperature aided in the accuracy of the tetrazolium test for both C. nobilior and C. walkeriana. Thus, the temperature during the test may be a determining factor in improving the visualization of viable seeds of these species.…”

Section: Introductionmentioning

confidence: 99%

Methodology of the tetrazolium test for identifying viable seeds of orchids of the genus Cattleya native to the Brazilian Cerrado

Ribeiro¹,

Sorgato²,

Soares³

et al. 2021

Semina: Ciênc. Agrár.

View full text Add to dashboard Cite

Orchids need measures for conservation of their species, among them the creation of seed banks. This study analyzes suitable methodologies to identify the viability of Cattleya seeds through the tetrazolium test. Seeds of Cattleya nobilior Rchb. and Cattleya walkeriana Gardn. were submitted to three preconditioning: no soaking (control); sucrose (10%); or distilled water. After 24 hours, they were submitted to tetrazolium solution with three types of conditioning: oven (40 °C); water bath (40 °C); or room temperature. Subsequently, the percentage of viable seeds (VS) was calculated and in vitro sowing was carried out. Germination percentage (G) was determined after 45 days. A completely randomized design was used for each species studied, with treatments arranged in a 3x3 factorial scheme (three preconditioning and three conditioning), with eight replicates of one tube each. The experiment was performed in duplicate. The highest viability values found in the tetrazolium test were close to those of germination (C. nobilior: VS = 88% e G = 97%; C. walkeriana: VS = 88% e G = 95%) for Cattleya species native to the Cerrado. The conditioning at ambient temperature had a greater effect on seed visualization for both species studied, suggesting that the tetrazolium test should be performed at this conditioning temperature.

show abstract

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Dolce

Medina

Terada

et al. 2020

Orchid Biology: Recent Trends &Amp; Challenges

View full text Add to dashboard Cite

Orchids are an important part of plant biodiversity on the planet due to their high variability among species and their habitats. South America presents more than thirty percent of all known orchid species, being Colombia, Ecuador, Brazil, Peru, and Bolivia between the richest countries in the world in terms of orchid biodiversity. Nevertheless, concerning to the orchid conservation status, in Colombia precisely orchids occupy the unlucky first place as the plant family with the highest number of threatened species. Similar situation is happening in the rest of the South American countries. The two main threats to orchid survival are both anthropogenic: the first one is deforestation, and the second largest threat to orchids is collection from the wild. One desirable action to safeguard these endangered species is to develop procedures that make possible their massive propagation, which should provide material for the eco-rehabilitation of specimens into their natural habitats, the exchange with other entities, the supply to orchid merchants for avoid extractions of nature, and the availability of material for future research. Likewise, the development of systems that allow the ex situ conservation of orchid germplasm is imperative. This chapter reviews the progresses of different in vitro approaches for orchid propagation and germplasm conservation, safeguarding the genetic biodiversity of these species. Several study cases are presented and described to exemplify the protocols developed in the Botanical Institute of Northeast (UNNE-CONICET) for propagating and long-term storing the germplasm of wild orchids from Argentina (Cattleya lundii, Cohniella cepula, Cohniella jonesiana, Gomesa bifolia, Aa achalensis, Cyrtopodium brandonianum, Cyrtopodium hatschbachii, Habenaria bractescens). Moreover, it has been attempted to put together most of the available literature on in vitro propagation and germplasm conservation for South American orchids using different explants and procedures. There are researches of good scientific quality that even cover critical insights into the physiology and factors affecting growth and development as well as storage of several orchid materials. Anyway, studies are still necessary to cover a mayor number of South American species as well as the use of selected material (clonal) for both propagation and conservation approaches.

show abstract

Refining the tetrazolium test for evaluation of Cattleya labiata and C. tigrina seeds viability

Cited by 15 publications

References 16 publications

Seed viability test of orchids native to the Brazilian Savanna1

Seed viability test of orchids native to the Brazilian Savanna1

Methodology of the tetrazolium test for identifying viable seeds of orchids of the genus Cattleya native to the Brazilian Cerrado

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Contact Info

Product

Resources

About