Reflectance confocal endomicroscope with optical axial scanning for in vivo imaging of the oral mucosa

Jabbour, Joey M.; Bentley, Julie; Malik, Bilal; Nemechek, John J.; Warda, John; Cuenca, Rodrigo; Cheng, Shannon; Jo, Javier A.; Maitland, Kristen C.

doi:10.1364/boe.5.003781

Cited by 18 publications

(23 citation statements)

References 21 publications

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“…The features observed most often in the mucosa epithelium were bright nuclei surrounded by a dark halo and bright cell membranes. Deeper in the epithelium, the cells were more spherical and smaller, the cell membranes were less distinct, and the nuclei were visible as dark structures in contrast to the bright nuclei seen in the upper layers , as confirmed by our data (Fig. a–b).…”

Section: Healthy Mucosasupporting

confidence: 89%

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The potential role of in vivo reflectance confocal microscopy for evaluating oral cavity lesions: a systematic review

Lucchese

Gentile

Romano

et al. 2016

J Oral Pathology Medicine

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Section: Healthy Mucosasupporting

confidence: 89%

“…Nine articles examined healthy mucosa at different oral sites and described its microscopic features on RCM (Table ) . The aim of these studies was to evaluate the efficacy of RCM for observing oral mucosa structures.…”

Section: Healthy Mucosamentioning

confidence: 99%

The potential role of in vivo reflectance confocal microscopy for evaluating oral cavity lesions: a systematic review

Lucchese

Gentile

Romano

et al. 2016

J Oral Pathology Medicine

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“…Currently, different optical imaging methods have been proposed to diagnose oral premalignant/malignant lesions because of their high-resolution and noninvasive natures, such as harmonic [9], multiphoton [10,11], confocal [12,13], photoacoustic [14] microscopies, as well as optical coherence tomography (OCT) [15,16]. For oral cavity imaging with microscopic techniques including harmonic, multiphoton, and confocal microscopies, the penetration depth is limited to hundreds micrometers, which may not be deep enough to investigate the existence of abnormal cells in the premalignant or malignant stage.…”

Section: Introductionmentioning

confidence: 99%

Noninvasive structural and microvascular anatomy of oral mucosae using handheld optical coherence tomography

Tsai¹,

Chen²,

Lee³

et al. 2017

Biomed. Opt. Express

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Abstract:In this study, we demonstrated the feasibility of using a handheld optical coherence tomography (OCT) for in vivo visualizations of the microstructural and microvascular features of various oral mucosal types. To scan arbitrary locations of the oral mucosa, a scanning probe was developed, composed of a probe body fabricated by a 3D printer, miniaturized two-axis galvanometer, relay lenses, and reflective prism. With a 3D printing technique, the probe weight and the system volume were greatly reduced, enabling the effective improvement of imaging artifacts from unconscious motion and system complexity. Additionally, in our design, the distal end of the probe can be switched to fit various oral conditions, and the optical parameters of the probe, such as the transverse resolution, working distance, and probe length can be easily varied. The results showed that the epithelium and lamina propria layers, as well as the fungiform papilla and salivary gland, were differentiated. Moreover, various microcirculation features at different mucosal sites were identified that are potentially effective indicators for the diagnosis of premalignant lesions. The demonstrated results indicate that the developed OCT system is a promising tool for noninvasive imaging of oral mucosae. 164-170 (2000). 5. L. L. Gleich, P. W. Biddinger, Z. P. Pavelic, and J. L. Gluckman, "Tumor angiogenesis in T1 oral cavity squamous cell carcinoma: Role in predicting tumor aggressiveness," Head Neck 18(4), 343-346 (1996). 6. L. L. Gleich, P. W. Biddinger, F. D. Duperier, and J. L. Gluckman, "Tumor angiogenesis as a prognostic indicator in T2-T4 oral cavity squamous cell carcinoma: A clinical-pathologic correlation," Head Neck 19(4), 276-280 (1997). 7. S. Silverman, Jr., "Early diagnosis of oral cancer," Cancer 62(8 Suppl), 1796-1799 (1988). cancer lesion with swept-source optical coherence tomography," J.

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“…Over the past few decades, confocal microscopes have been widely used for bench‐top biological investigations , as well as for clinical applications such as in vivo microendoscopy and point‐of‐care pathology . By means of point illumination and pinhole detection, confocal microscopy effectively rejects out‐of‐focus and multiply scattered light, providing users with high‐contrast images of three‐dimensional tissue microstructure .…”

Section: Introductionmentioning

confidence: 99%

Bessel‐beam illumination in dual‐axis confocal microscopy mitigates resolution degradation caused by refractive heterogeneities

Chen

Glaser

Liu

2016

Journal of Biophotonics

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One of the main challenges for laser-scanning microscopy of biological tissues with refractive heterogeneities is the degradation in spatial resolution that occurs as a result of beam steering and distortion. This challenge is particularly significant for dual-axis confocal (DAC) microscopy, which achieves improved spatial-filtering and optical-sectioning performance over traditional confocal microscopy through off-axis illumination and collection of light with low-numerical aperture (NA) beams that must intersect precisely at their foci within tissues. DAC microscope image quality is sensitive to positional changes and distortions of these illumination- and collection-beam foci. Previous studies have shown that Bessel beams display improved positional stability and beam quality than Gaussian beams when propagating through tissues with refractive heterogeneities, which suggests that Bessel-beam illumination may enhance DAC microscopy of such tissues. Here, we utilize both Gaussian and Bessel illumination in a point-scanned DAC microscope and quantify the resultant degradation in resolution when imaging within heterogeneous optical phantoms and fresh tissues. Results indicate that DAC microscopy with Bessel illumination exhibits reduced resolution degradation from microscopic tissue heterogeneities compared to DAC microscopy with conventional Gaussian illumination.

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Reflectance confocal endomicroscope with optical axial scanning for in vivo imaging of the oral mucosa

Cited by 18 publications

References 21 publications

The potential role of in vivo reflectance confocal microscopy for evaluating oral cavity lesions: a systematic review

The potential role of in vivo reflectance confocal microscopy for evaluating oral cavity lesions: a systematic review

Noninvasive structural and microvascular anatomy of oral mucosae using handheld optical coherence tomography

Bessel‐beam illumination in dual‐axis confocal microscopy mitigates resolution degradation caused by refractive heterogeneities

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