Reflections on the clonal-selection theory

Cohn, Melvin; Mitchison, N A; Paul, William E.; Silverstein, Arthur M.; Talmage, David W.; Weigert, Martin

doi:10.1038/nri2177

Cited by 50 publications

(34 citation statements)

References 63 publications

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“…Another important concept in early adaptive immunity is that of clonal expansion [18], and shedding at the cell surface influences lymphocyte proliferation by modulating ligand receptor interactions. LAG3 (Lymphocyte Activation Gene 3) inhibits T-cell proliferation, and its cleavage by ADAM10 and ADAM17 alters clonal expansion ( Figure 2a).…”

Section: Metalloproteinases Facilitate Lymphocyte Development and Promentioning

confidence: 99%

Clipping, shedding and RIPping keep immunity on cue

Murphy

Murthy

Khokha

2008

Trends in Immunology

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Section: Metalloproteinases Facilitate Lymphocyte Development and Promentioning

confidence: 99%

Clipping, shedding and RIPping keep immunity on cue

Murphy

Murthy

Khokha

2008

Trends in Immunology

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“…In the case of B lymphocytes, antigen receptors can be further diversified in an antigen-dependent manner by somatic hypermutation of clonally expanded lymphocytes. 2,3 Thus, the basic unit of the B cell repertoire is the idiotype, which refers to a unique antigen receptor structure (H and L chain pair) with unique epitope specificity. One or more idiotypes derived from the same VDJ recombination event and H C L pairing represents a clonotype.…”

Section: Introductionmentioning

confidence: 99%

Reconstructing and mining the B cell repertoire with ImmunediveRsity

Cortina-Ceballos

Godoy-Lozano

Sámano-Sánchez

et al. 2015

mAbs

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(2015) Reconstructing and mining the B cell repertoire with ImmunediveRsity , mAbs, 7:3, 516-524, DOI: 10.1080/19420862.2015 To link to this article: https://doi.org/10. 1080/19420862.2015 Keywords: high-throughput sequencing, Ig repertoire, CDR3, data mining Abbreviations: HEL, hen egg lysozyme; CDRH3, heavy chain complementarity determining region 3; Rep-Seq, repertoire sequencing; SHM, somatic hypermutation.The B cell antigen receptor repertoire is highly diverse and constantly modified by clonal selection. High-throughput DNA sequencing (HTS) of the lymphocyte repertoire (Rep-Seq) represents a promising technology to explore such diversity ex-vivo and assist in the identification of antigen-specific antibodies based on molecular signatures of clonal selection. Therefore, integrative tools for repertoire reconstruction and analysis from antibody sequences are needed. We developed ImmunediveRity, a stand-alone pipeline primarily based in R programming for the integral analysis of B cell repertoire data generated by HTS. The pipeline integrates GNU software and in house scripts to perform quality filtering, sequencing noise correction and repertoire reconstruction based on V, D and J segment assignment, clonal origin and unique heavy chain identification. Post-analysis scripts generate a wealth of repertoire metrics that in conjunction with a rich graphical output facilitates sample comparison and repertoire mining. Its performance was tested with raw and curated human and mouse 454-Roche sequencing benchmarks providing good approximations of repertoire structure. Furthermore, ImmunediveRsity was used to mine the B cell repertoire of immunized mice with a model antigen, allowing the identification of previously validated antigen-specific antibodies, and revealing different and unexpected clonal diversity patterns in the post-immunization IgM and IgG compartments. Although ImmunediveRsity is similar to other recently developed tools, it offers significant advantages that facilitate repertoire analysis and repertoire mining. ImmunediveRsity is open source and free for academic purposes and it runs on 64 bit GNU/Linux and MacOS. Available at: https://bitbucket.org/ImmunediveRsity/immunediversity/

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“…В основе обсуждаемой парадигмы лежит теория клональной селекции, предложенная Бернетом в се-редине прошлого века, активно дискутировавшаяся и модифицированная современными учеными [60,61]. Одним из важнейших постулатов этой теории остается утверждение о том, что В-клеточный от-вет, приводящий к продукции антител, вызывается не любым чужеродным антигеном, а лишь теми, в контексте молекулярной структуры которых ВкР антителопродуцента могут отличить «свое» от «не своего».…”

Section: антигенность и иммуногенность Hla-молекул парадигма «не своunclassified

The mechanism of humoral immune response to allogeneic organ transplantation

Berkos

Николаев

2017

RJTAO

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1 ФГБУ «Российский НИИ гематологии и трансфузиологии ФМБА России», Санкт-Петербург, Российская Федерация 2 ФГБУ «Северо-Западный федеральный медицинский исследовательский центр» Минздрава России, Санкт-Петербург, Российская Федерация Проблема гуморального, антителоопосредованного отторжения донорского органа остается крайне ак-туальной. Главной мишенью антител в основном являются донорские HLA-антигены (Human Leucocyte Antigens), экспрессируемые, в частности, клетками сосудистого эндотелия трансплантата. В данном обзоре рассматриваются механизмы развития гуморального аллоиммунитета, в основе которых лежит распознавание В-клетками реципиента эпитопов донорских HLA-молекул и созревание аффинности В-клеточных рецепторов в герминативных центрах периферической лимфатической системы. Учет по-казателей эпитопной нагрузки и кросс-реактивности при анализе HLA-совместимости донора и реципи-ента играет важную роль в профилактике гуморального отторжения аллогенного трансплантата.Ключевые слова: гуморальное отторжение, аллогенный трансплантат, HLAантигены, Вклеточные рецепторы, герминативные центры, эпитопы, кроссреактивность.

show abstract

Reflections on the clonal-selection theory

Cited by 50 publications

References 63 publications

Clipping, shedding and RIPping keep immunity on cue

Clipping, shedding and RIPping keep immunity on cue

Reconstructing and mining the B cell repertoire with ImmunediveRsity

The mechanism of humoral immune response to allogeneic organ transplantation

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