Refraction Imaging and Histologic Correlation in Excised Tissue from a Normal Human Lung

Kono, Michio; Ohbayashi, Chiho; Yamasaki, Katsuhito; Ohno, Yoshiharu; Adachi, Shuji; Sugimura, Kazuro; Suzuki, Yoshio

doi:10.1016/s1076-6332(03)80769-9

Cited by 7 publications

(6 citation statements)

References 5 publications

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“…Lung tissue is an ideal specimen for phase‐contrast images using synchrotron radiation because of the large difference of refractive index between air and soft tissue (Liu et al, ). There have been several studies that discussed the potential usefulness of synchrotron radiation for diagnostic radiology and medical imaging of lung disease (Bayat et al, ; Jheon et al, ; Kono et al, ; Liu et al, ). In our study, we obtained microscopic images of CT using synchrotron radiation and CCD cameras from human lung specimen containing adenocarcinoma.…”

Section: Discussionmentioning

confidence: 99%

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Synchrotron tomographic images from human lung adenocarcinoma: Three‐dimensional reconstruction and histologic correlations

Han

Chang

et al. 2017

Microscopy Res & Technique

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High-resolution tomographic images using synchrotron X-rays are expected to provide detailed reflection of microstructures, thereby allowing for the examination of histologic structures without destruction of the specimen. This study aims to evaluate the synchrotron tomographic images of mixed ground-glass opacity excised on 5-mm sections in comparison to pathologic examination. The Institutional Review Board of our institute approved this retrospective study, and written informed consent was obtained from each patient whose lung tissue would be used. Obtained lung cancer specimens were brought to the multiple Wiggler 6C beam line at the Pohang Light Source (PLS-II) in Korea, and phase contrast X-ray images were obtained in November 2016. The X-ray emanated from a bending magnet of the electron storage ring with electron energy of 3 GeV, and a typical beam current was 320 mA. Reconstructed tomographic images were compared with images from histologic slides obtained from the same samples. Pulmonary microstructures including terminal bronchioles, alveolar sacs, and vasculature were identified with phase contrast X-ray images. Images from normal lung tissue and mixed ground-glass opacity were clearly distinguishable. Hyperplasia of the interalveolar septum and dysplasia of microstructure were clearly identified. The imaging findings correlated well with hematoxylin-eosin stained specimens. Tomographic images using synchrotron radiation have the potential for clinical applications. With refinement, this technique may become a diagnostic tool for detection of lung cancer.

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Section: Discussionmentioning

confidence: 99%

“…Prior studies have examined small animals and human lung tissue using synchrotron X-rays (Jheon, Youn, Kim, Choi, & Kim, 2006;Kono et al, 2001;Liu, Sun, Guan, Zhang, & Xu, 2006). In this study, human lung nodule specimens (diagnosed as lung adenocarcinoma) were examined using synchrotron radiation and compared with pathologic examination.…”

Section: Introductionmentioning

confidence: 99%

Synchrotron tomographic images from human lung adenocarcinoma: Three‐dimensional reconstruction and histologic correlations

Han

Chang

et al. 2017

Microscopy Res & Technique

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“…Enlargement of the visual image obtained from the scintillator using a 203 microscope objective provides 10 times higher spatial resolution than simply refraction-enhanced imaging of lung tissue as compared with previous studies (Kono et al, 2001;Yagi et al, 1999). In addition, a thin scintillator with thickness of 20 lm was used to improve spatial resolution of ejected photons.…”

Section: Discussionmentioning

confidence: 99%

“…Although Kono et al (2001) and Yagi et al (1999) obtained static refraction images from resected tissue of human and mouse lung, we were able to produce realtime moving images of a single alveolus, its network, and the perialveolar microcirculation, using ventilation technique. Thus, SR imaging may also provide functional information based on the series of such moving images.…”

Section: Discussionmentioning

confidence: 99%

High-resolution X-ray refraction imaging of rat lung and histological correlations

Jheon

Youn

Kim

et al. 2006

Microsc. Res. Tech.

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This study was performed to observe microstructures of the rat lung, using a synchrotron radiation beam and to compare findings with histological observations. X-ray refraction images from ex-vivo ventilating rat lung were obtained with an 8 KeV monochromatic beam and 20-mum thick CsI(Tl) scintillation crystal. The visual image was magnified using a 20x microscope objective and captured using an analog CCD camera. Obtained images were compared with conventional light microscopic findings from the same tissue. Pulmonary microstructures, including alveolar ducts, alveolar sacs, alveoli, alveolar walls, and perialveolar capillary networks were clearly identified with spatial resolution of as much as 1.2 mum and had good correlation with conventional light microscopic findings. The shape of alveoli appeared more round in SR images than in the light microscopic images. The results suggest that X-ray microscopy study of the lung using synchrotron radiation demonstrates the potential for clinically relevant microstructure of lung tissue without sectioning and fixation.

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“…Imaging experiment which has been carried out in 2 mm-thick sections of excised normal human lung [18], mouse and rabbit lungs [19] at BL20B2-H3 in the BIC revealed that lungs can be rendered highly visible using the DEI. This technique was also applied to imaging for quality control investigation of opaque objects such as mammographic phantoms [20].…”

Section: Diffraction-enhanced Imaging (Dei)mentioning

confidence: 99%

Clinical step onward with X-ray dark-field imaging and perspective view of medical applications of synchrotron radiation in Japan

Ando

Hashimoto

Hashizume

et al. 2005

Nuclear Instruments and Methods in Physics Research Section A:

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Refraction Imaging and Histologic Correlation in Excised Tissue from a Normal Human Lung

Cited by 7 publications

References 5 publications

Synchrotron tomographic images from human lung adenocarcinoma: Three‐dimensional reconstruction and histologic correlations

Synchrotron tomographic images from human lung adenocarcinoma: Three‐dimensional reconstruction and histologic correlations

High-resolution X-ray refraction imaging of rat lung and histological correlations

Clinical step onward with X-ray dark-field imaging and perspective view of medical applications of synchrotron radiation in Japan

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