Reg IV is differently expressed in enteroendocrine cells of human small intestine and colon

Heiskala, Kukka; Andersson, Leif C.

doi:10.1016/j.regpep.2013.03.007

Cited by 5 publications

(5 citation statements)

References 51 publications

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“…58 REG4 has been further suggested as a biomarker for the discrimination of CD from UC patients, as it showed differential expression in the inflamed and uninflamed biopsies of six CD patients when compared with six UC patients. 59,60 This study also detected higher REG4 plasma levels in CD vs control and UC vs control, supporting the strategy of applying high-throughput proteomics technology to larger patient populations to identify plasma protein biomarkers for diagnostics.…”

Section: Discussionsupporting

confidence: 65%

High-Throughput Identification of the Plasma Proteomic Signature of Inflammatory Bowel Disease

Narzo

Brodmerkel

Telesco

et al. 2018

Journal of Crohn's and Colitis

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Background The molecular aetiology of inflammatory bowel disease [IBD] and its two subtypes, ulcerative colitis [UC] and Crohn’s disease [CD], have been carefully investigated at genome and transcriptome levels. Recent advances in high-throughput proteome quantification has enabled comprehensive large-scale plasma proteomics studies of IBD. Methods The study used two cohorts: [1] The CERTIFI-cohort: 42 samples from the CERTIFI trial of anti-TNFα–refractory CD patients; [2] the PROgECT-UNITI-HCs cohort: 46 UC samples of the PROgECT study, 84 CD samples of the UNITI I and UNITI II studies, and 72 healthy controls recruited in Mount Sinai Hospital, New York, USA. The plasma proteome for these two cohorts was quantified using high-throughput platforms. Results For the PROgECT-UNITI-HCs cohort, we measured a total of 1310 proteins. Of these, 493 proteins showed different plasma levels in IBD patients to the plasma levels in controls at 10% false discovery rate [FDR], among which 11 proteins had a fold change greater than 2. The proteins upregulated in IBD were associated with immunity functionality, whereas the proteins downregulated in IBD were associated with nutrition and metabolism. The proteomic profiles were very similar between UC and CD. In the CERTIFI cohort, 1014 proteins were measured, and it was found that the plasma protein level had little correlation with the blood or intestine transcriptomes. Conclusions We report the largest proteomics study to date on IBD and controls. A large proportion of plasma proteins are altered in IBD, which provides insights into the disease aetiology and indicates a potential for biomarker discovery.

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Section: Discussionsupporting

confidence: 65%

High-Throughput Identification of the Plasma Proteomic Signature of Inflammatory Bowel Disease

Narzo

Brodmerkel

Telesco

et al. 2018

Journal of Crohn's and Colitis

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“…Of five genes with decreased expression in GF mice, four were (functionally) linked to immune cells [ Cd74 , glucosaminyl (N-acetyl) transferase 1, core ( Gcnt1 ), 2H2-Ab1 , and H2-Ea-ps ]. The fifth gene, regenerating islet-derived family, member 4 ( Reg4 ), encodes small secreted lectin that is expressed in the gastrointestinal tract especially in enteroendocrine cells [66,67]. REG4 functions as an anti-apoptotic tissue regeneration-promoting factor [68].…”

Section: Resultsmentioning

confidence: 99%

Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice

Janečková

Kostovcikova

Švec

et al. 2019

IJMS

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Commensal microbiota contribute to gut homeostasis by inducing transcription of mucosal genes. Analysis of the impact of various microbiota on intestinal tissue provides an important insight into the function of this organ. We used cDNA microarrays to determine the gene expression signature of mucosa isolated from the small intestine and colon of germ-free (GF) mice and animals monoassociated with two E. coli strains. The results were compared to the expression data obtained in conventionally reared (CR) mice. In addition, we analyzed gene expression in colon organoids derived from CR, GF, and monoassociated animals. The analysis revealed that the complete absence of intestinal microbiota mainly affected the mucosal immune system, which was not restored upon monoassociation. The most important expression changes observed in the colon mucosa indicated alterations in adipose tissue and lipid metabolism. In the comparison of differentially expressed genes in the mucosa or organoids obtained from GF and CR mice, only six genes were common for both types of samples. The results show that the increased expression of the angiopoietin-like 4 (Angptl4) gene encoding a secreted regulator of lipid metabolism indicates the GF status.

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“…One consistently downregulated gene was REG4 , a 17 kD secreted C-type lectin, expressed in selected enteroendocrine cells throughout the GIT and some goblet cells[ 66 ]. The functional role of REG4 is still unclear though it appears to be coexpressed with various enteroendocrine hormones.…”

Section: Discussionmentioning

confidence: 99%

Glycosylation-related gene expression in HT29-MTX-E12 cells upon infection by Helicobacter pylori

Cairns¹,

Gupta²,

Naughton³

et al. 2017

WJG

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AIMTo identify glycosylation-related genes in the HT29 derivative cell line, HT29-MTX-E12, showing differential expression on infection with Helicobacter pylori (H. pylori).METHODSPolarised HT29-MTX-E12 cells were infected for 24 h with H. pylori strain 26695. After infection RNA was isolated from both infected and non-infected host cells. Sufficient infections were carried out to provide triplicate samples for microarray analysis and for qRT-PCR analysis. RNA was isolated and hybridised to Affymetrix arrays. Analysis of microarray data identified genes significantly differentially expressed upon infection. Genes were grouped into gene ontology functional categories. Selected genes associated with host glycan structure (glycosyltransferases, hydrolases, lectins, mucins) were validated by real-time qRT-PCR analysis.RESULTSInfection of host cells was confirmed by the isolation of live bacteria after 24 h incubation and by PCR amplification of bacteria-specific genes from the host cell RNA. H. pylori do not survive incubation under the adopted culture conditions unless they associate with the adherent mucus layer of the host cell. Microarray analysis identified a total of 276 genes that were significantly differentially expressed (P < 0.05) upon H. pylori infection and where the fold change in expression was greater than 2. Six of these genes are involved in glycosylation-related processes. Real-time qRT-PCR demonstrated significant downregulation (1.8-fold, P < 0.05) of the mucin MUC20. REG4 was heavily expressed and significantly downregulated (3.1-fold, P < 0.05) upon infection. Gene ontology analysis was consistent with previous studies on H. pylori infection.CONCLUSIONGene expression data suggest that infection with H. pylori causes a decrease in glycan synthesis, resulting in shorter and simpler glycan structures.

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Reg IV is differently expressed in enteroendocrine cells of human small intestine and colon

Cited by 5 publications

References 51 publications

High-Throughput Identification of the Plasma Proteomic Signature of Inflammatory Bowel Disease

High-Throughput Identification of the Plasma Proteomic Signature of Inflammatory Bowel Disease

Unique Gene Expression Signatures in the Intestinal Mucosa and Organoids Derived from Germ-Free and Monoassociated Mice

Glycosylation-related gene expression in HT29-MTX-E12 cells upon infection by Helicobacter pylori

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