Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet‐rich plasma and dental pulp cells

Zhu, Wenhao; Zhu, Xiaofei; Huang, George T.‐J.; Cheung, Gary; Dissanayaka, Waruna Lakmal; Zhang, Chengfei

doi:10.1111/iej.12087

Cited by 98 publications

(94 citation statements)

References 34 publications

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“…It has been reported that PRP plays a role in promoting the healing of wounds of hard and soft tissues and in the regeneration of osseous tissues. Researchers have applied PRP to such clinical treatment fields as orthopedics, oral cavity dental implants, and periodontal surgery (Zhu et al, 2012(Zhu et al, , 2013. Studies have found that that the washed platelets (WPLTs) obtained from PRP following removal of the plasma play a significant role in promoting the proliferation of rat dental pulp cells, suggestion the potential for application of improved PRP towards retention treatment of vital dental pulp and even towards regeneration treatment of dental pulp tissues (Orhan et al, 2012;Wang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells

2015

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ABSTRACT. We examined the effects of washed platelets (WPLTs) and platelet-rich plasma (PRP) on the proliferation and mineralization of rat dental pulp cells. Rat dental pulp cells were separated, cultured, and identified. Medium containing 1, 10, 100, or 500 mL/L PRP or WPLTs was added to 4th generation cells. The MTS method was used to determine cell proliferation. Alizarin red staining was used to observe the formation of mineralized nodules after cell mineralization and induction for 10 and 20 days under different culture conditions, and the areas of the mineralized nodules formed 20 days after induction were computed. The addition of 1, 10, and 100 mL/L WPLTs or PRP significantly promoted rat dental pulp cell proliferation (P < 0.05) whereas 500 mL/L WPLTs or PRP had no significant effect (P > 0.05). Under the same concentrations, no significant differences on cell proliferation were observed between WPLT and PRP treatments (P > 0.05 in all groups). After 10 days mineralization and culture, the 100 and 500 mL/L WPLT and PRP group positive nodule rates were significantly higher than those of the low concentration and the control groups (P < 0.05). After 20 days, the areas of the mineralized nodules formed in the 100 and 500 mL/L WPLT and 9487 Effects of WPLTs and PRP on rat dental pulp cells ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 9486-9496 (2015) PRP groups were significantly larger than those in the control group (P < 0.05). These results demonstrate that both WPLTs and PRP are equally able to significantly promote the proliferation and calcification of rat dental pulp cells under a certain range of concentrations.

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Section: Introductionmentioning

confidence: 99%

Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells

2015

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“…[n=NR] Zhu et al 13 [n=10C] Yoo et al 15 [n=16C] Londero et al 17 [n=17C] Torabinejad et al 19 [n=2T]…”

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confidence: 99%

Histological assessment of regenerative endodontic treatment in animal studies with different scaffolds: A systematic review

Altaii

Richards

Rossi‐Fedele

2017

Dental Traumatology

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“…68 Bir başka çalışmada, DPSC ile TZP'nin birlikte uygulandığı grupla kıyaslandığında, TZP'nin tek başına kullanımının kök kanal duvar kalınlaşmasını ve mineralize doku oluşumunu daha az sağladığı belirtilmiştir. 69 Zhang ve ark. 70 TZP uygulamasının, apikal dokuda kanamanın bulunmadığı klinik olgular için bir seçenek olabileceğini belirtmişlerdir.…”

Section: Kan Pıhtısı Kullanımı Yöntemiunclassified

Traditional and Current Treatment Methods from Apexification to Apexogenesis

Ceyhan¹,

Akdik²

2018

EÜ Dişhek Fak Derg

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ÖZETBu derlemenin amacı; herhangi bir sebeple canlılığını kaybetmiş, olgunlaşmamış, genç daimi dişlerin gelişimlerinin tamamlanabilmesi için çocuk diş hekimliğinde rutin olarak kullanılan geleneksel tedavi yöntemleri ve günümüzde sıklıkla kullanılmaya başlanan rejeneratif yöntemleri ele almak ve konu ile ilgili yapılmış çalışmaları sunarak gelecekteki klinik uygulamalara katkı sağlamaktır. Nekrotik genç daimi dişlerin kök gelişiminin sağlanması için farklı materyaller kullanılarak uygulanan apeksifikasyon tedavisinden uzun yıllardır yararlanılmaktadır. Bu yöntemin bazı dezavantajları nedeniyle rejeneratif tedavi yöntemleri gündeme gelmeye başlamıştır. Rejeneratif prosedürlerin temelini oluşturan doku mühendisliği, fonksiyonunu kaybetmiş doku ya da organların yeniden restorasyonuna odaklanmakta olup bu konuda yapılan çalışmalar diş hekimliğinin birçok alanında ümit verici sonuçlar elde edildiğini göstermektedir. İlerleyen teknoloji, yüksek hızlı el aletlerinin geliştirilmesinden biyolojik onarım materyallerine kadar diş hekimliği uygulamaları üzerinde önemli, süregelen bir etkiye sahiptir. Doku mühendisliği uygulamalarının günümüzde kullanılan diş tedavilerine alternatif olabilmesi açısından, hekimlerin bu uygulamalar hakkında detaylı bilgilere sahip olmaları ve araştırmalar yaparak uzun dönem sonuçlarını sunmaları önem arz etmektedir.

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Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet‐rich plasma and dental pulp cells

Abstract: A combination of DPCs + PRP increased vital tissue regeneration within the root canals of immature teeth associated with apical periodontitis.

Cited by 98 publications

References 34 publications

Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells

Effects of washed platelets vs platelet-rich plasma on the proliferation and mineralization of rat dental pulp cells

Histological assessment of regenerative endodontic treatment in animal studies with different scaffolds: A systematic review

Traditional and Current Treatment Methods from Apexification to Apexogenesis

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