Regeneration of mandibular defects using adipose tissue mesenchymal stromal cells in combination with human serum-derived scaffolds

Peña, Ignacio; Álvarez-Viejo, Marı́a; Alonso-Montes, Cristina; Menéndez-Menéndez, Yolanda; Álvarez, Fernando Gutiérrez; Rodríguez, J.C. De Vicente; Otero, Jesús María Garagorri; Meana, Álvaro

doi:10.1016/j.jcms.2016.06.012

Cited by 8 publications

(6 citation statements)

References 46 publications

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“…19 STRO-1 has been associated with increased osteogenic capacity 20 and is a marker of stem cells. 21 Studies have demonstrated STRO-1 expression around vessels as well in the pulp 22 and also in pyogenic granuloma and POF. 23 In contrast with the literature, this study found less STRO-1 expression near mineralized tissue, suggesting that these few stained cells have not yet undergone differentiation into osteoblasts or cementoblasts.…”

Section: Discussionmentioning

confidence: 99%

The role of Osterix protein in the pathogenesis of peripheral ossifying fibroma

et al. 2017

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Peripheral ossifying fibroma (POF) is a reactive lesion of oral tissues, associated with local factors such as trauma or presence of dental biofilm. POF treatment consists of curettage of the lesion combined with root scaling of adjacent teeth and/or removal of other sources of irritants. This study aimed to analyze the clinical and pathological features of POF and to investigate the immunoexpression of Osterix and STRO-1 proteins. Data such as age, gender, and size were obtained from 30 cases of POF. Microscopic features were assessed by conventional light microscopy using hematoxylin-eosin staining and immunohistochemical markers, and by polarized light microscopy using Picrosirius red staining. The age range was 11-70 years and 70% of the patients were female. Moreover, the size of POF varied from 0.2 to 5.0 cm; in 43.33% of the cases, the mineralized content consisted exclusively of bony trabeculae. The immunohistochemical analysis showed nuclear staining for Osterix in 63% and for STRO-1 in 20% of the cases. Mature collagen fibers were observed in mineralized tissue in 76.67% of the cases. The clinical and microscopic features observed were in agreement with those described in the literature. Osterix was overexpressed, while STRO-1 was poorly expressed. Osterix was expressed particularly in cells entrapped in and around mineralized tissue, indicating the presence of a stimulus that triggers the differentiation of these cells into osteoblasts or cementoblasts, i.e., cells that produce mineralized tissue. Based on our results, Osterix may play a role in the pathogenesis of POF.

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Section: Discussionmentioning

confidence: 99%

The role of Osterix protein in the pathogenesis of peripheral ossifying fibroma

et al. 2017

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“…Криогели на белковой основе применяют в качестве пористых подложек для объемного культивирования ММСК и последующей имплантации полученных образцов той или иной ткани благодаря их макропористой морфологии и способности к биодеградации (4,9). Имеются данные о применении для этой цели разных криогелей на основе желатина (17), сывороточного альбумина (21) и суммарного белка сыворотки крови (18)(19)(20). Представляло интерес протестировать криогели в связи с разработкой в нашей лаборатории метода создания «культурального мяса в пробирке» (10).…”

Section: рис 2 микрофотографии срезов широкопористых матриц на осноunclassified

“…В серии экспериментов было продемонстрировано, что криогели на белковой основе (сывороточный альбумин, суммарный белок сыворотки крови) благодаря макропористой морфологии могут быть использованы в качестве пористых подложек для культивирования клеток (18,19). Основными критериями биологически совместимой матрицы должны быть отсутствие цитотоксичности, поддержание адгезии, миграции, пролиферации или дифференцировки помещенных на ее поверхность клеток (20)(21)(22), а также оптимальная для дальнейшего применения механическая проч-ность и биорезорбируемость (23).…”

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Maintenance of Multipotent Mesenchymal Stem Cells of Farm Animals in Cryogels Based on Naturally-Derived Polymers

Korovina¹

2019

S-h. biol.

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* Работа выполнена в рамках НИР ¹ 0578-2018-0006 «Создание новых клеточных систем с заданными свойствами на основе стволовых клеток млекопитающих, в том числе сельскохозяйственных животных для ветеринарии, вирусологии и биотехнологии».

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“…For bone tissue engineering (BTE) applications, the efficacy of BMSC in regenerating bone has been investigated in many in vivo studies (Dang et al 2017;Kanczler et al 2008;Koob et al 2011;Zong et al 2010). Similarly, the in vivo bone regenerative capacity of ASC has been studied (Levi et al 2010;Peña González et al 2016;Yoon et al 2007). Previous studies have also compared the in vivo capacity of ASC and BMSC in bone regeneration to evaluate the effect of these MSC for BTE (Bothe et al 2018;Degano et al 2008;Freitas et al 2019;Jo et al 2013;Kang et al 2012;Kargozar et al 2018;Kim et al 2014;Lin et al 2009;Niemeyer et al 2010;Stockmann et al 2012;Walmsley et al 2016;Wen et al 2013;Xu et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Comparison of bone regenerative capacity of donor-matched human adipose–derived and bone marrow mesenchymal stem cells

et al. 2020

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Adipose-derived stem cells (ASC) have been used as an alternative to bone marrow mesenchymal stem cells (BMSC) for bone tissue engineering. However, the efficacy of ASC in bone regeneration in comparison with BMSC remains debatable, since inconsistent results have been reported. Comparing ASC with BMSC obtained from different individuals might contribute to this inconsistency in results. Therefore, this study aimed to compare the bone regenerative capacity of donor-matched human ASC and BMSC seeded onto poly(l-lactide-co-ε-caprolactone) scaffolds using calvarial bone defects in nude rats. First, donor-matched ASC and BMSC were seeded onto the co-polymer scaffolds to evaluate their in vitro osteogenic differentiation. Seeded scaffolds and scaffolds without cells (control) were then implanted in calvarial defects in nude rats. The expression of osteogenesis-related genes was examined after 4 weeks. Cellular activity was investigated after 4 and 12 weeks. Bone formation was evaluated radiographically and histologically after 4, 12, and 24 weeks. In vitro, ASC and BMSC demonstrated mineralization. However, BMSC showed higher alkaline phosphatase activity than ASC. In vivo, human osteogenesis–related genes Runx2 and collagen type I were expressed in defects with scaffold/cells. Defects with scaffold/BMSC had higher cellular activity than defects with scaffold/ASC. Moreover, bone formation in defects with scaffold/BMSC was greater than in defects with scaffold/ASC, especially at the early time-point. These results suggest that although ASC have the potential to regenerate bone, the rate of bone regeneration with ASC may be slower than with BMSC. Accordingly, BMSC are more suitable for bone regenerative applications.

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Regeneration of mandibular defects using adipose tissue mesenchymal stromal cells in combination with human serum-derived scaffolds

Cited by 8 publications

References 46 publications

The role of Osterix protein in the pathogenesis of peripheral ossifying fibroma

The role of Osterix protein in the pathogenesis of peripheral ossifying fibroma

Maintenance of Multipotent Mesenchymal Stem Cells of Farm Animals in Cryogels Based on Naturally-Derived Polymers

Comparison of bone regenerative capacity of donor-matched human adipose–derived and bone marrow mesenchymal stem cells

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