Regenerative Wound Surgery: Practical Application of Regenerative Medicine in the <scp>OR</scp>

Gillette, Brian M.; Criscitelli, Theresa; Howell, Robert J.; Woods, Jon S.; Acerra, Michael; Gorenstein, Scott

doi:10.1002/aorn.12615

Cited by 2 publications

(1 citation statement)

References 43 publications

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“…These products and technologies have different indications and action mechanisms at different stages of the healing process. Despite their benefits, all the methods mentioned have high cost, and the effectiveness of these treatments is still debated within the scientific community 12 , 13 . Currently, there is no definitive and effective treatment for chronic skin wounds on the market.…”

Section: Introductionmentioning

confidence: 99%

Tissue healing changes on wounds in rats after treatment with Hancornia speciosa latex in cream-gel formulation

D’Abadia

Lemes²,

Reis

et al. 2022

Acta Cir. Bras.

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Purpose: Hancornia speciosa latex has shown pharmacological potential in wound healing processes due to its angiogenic, osteogenic, and anti-inflammatory activities. The aims of this study were to carry out a cream-gel formulation with 5, 10 and 25% of H. speciosa serum latex and to evaluate its potential to stimulate the skin regeneration in rats’ wounds. Methods: One hundred and twenty rats were divided into five groups: neutral control with saline (G1), cream-gel based on H. speciosa latex serum at 5% m/v (G2), cream-gel at 15% m/v (G3), cream-gel at 25% m/v (G4), and cream-gel (G5). The animals were euthanized at three, seven, 14 and 21 days after the injury induction, and some parameters were analyzed: wound contraction, necrosis, fibrin, polymorphonuclear and mononuclear infiltrates, fibroblast, angiogenesis, hemorrhage, and collagen. Results: The therapeutic treatment with cream-gel at 15 and 25% is beneficial in the inflammatory phase of healing processes since it increased the angiogenesis and proliferation of mononuclear infiltrations in wounds. Regarding wound contraction, the treatment with cream-gel (5 and 15%) induced a higher rate of contraction in the proliferative phase. The 15% cream-gel formulation stimulated a greater production of collagen in the injured tissues. Conclusions: H. speciosa cream-gel is a low-cost herbal medicine which can aid in tissue repair.

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Section: Introductionmentioning

confidence: 99%

Tissue healing changes on wounds in rats after treatment with Hancornia speciosa latex in cream-gel formulation

D’Abadia

Lemes²,

Reis

et al. 2022

Acta Cir. Bras.

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Bioactive glasses and electrospun composites that release cobalt to stimulate the HIF pathway for wound healing applications

et al. 2021

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Background Bioactive glasses are traditionally associated with bonding to bone through a hydroxycarbonate apatite (HCA) surface layer but the release of active ions is more important for bone regeneration. They are now being used to deliver ions for soft tissue applications, particularly wound healing. Cobalt is known to simulate hypoxia and provoke angiogenesis. The aim here was to develop new bioactive glass compositions designed to be scaffold materials to locally deliver pro-angiogenic cobalt ions, at a controlled rate, without forming an HCA layer, for wound healing applications. Methods New melt-derived bioactive glass compositions were designed that had the same network connectivity (mean number of bridging covalent bonds between silica tetrahedra), and therefore similar biodegradation rate, as the original 45S5 Bioglass. The amount of magnesium and cobalt in the glass was varied, with the aim of reducing or removing calcium and phosphate from the compositions. Electrospun poly(ε-caprolactone)/bioactive glass composites were also produced. Glasses were tested for ion release in dissolution studies and their influence on Hypoxia-Inducible Factor 1-alpha (HIF-1α) and expression of Vascular Endothelial Growth Factor (VEGF) from fibroblast cells was investigated. Results Dissolution tests showed the silica rich layer differed depending on the amount of MgO in the glass, which influenced the delivery of cobalt. The electrospun composites delivered a more sustained ion release relative to glass particles alone. Exposing fibroblasts to conditioned media from these composites did not cause a detrimental effect on metabolic activity but glasses containing cobalt did stabilise HIF-1α and provoked a significantly higher expression of VEGF (not seen in Co-free controls). Conclusions The composite fibres containing new bioactive glass compositions delivered cobalt ions at a sustained rate, which could be mediated by the magnesium content of the glass. The dissolution products stabilised HIF-1α and provoked a significantly higher expression of VEGF, suggesting the composites activated the HIF pathway to stimulate angiogenesis.

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Regenerative Wound Surgery: Practical Application of Regenerative Medicine in the OR

Cited by 2 publications

References 43 publications

Tissue healing changes on wounds in rats after treatment with Hancornia speciosa latex in cream-gel formulation

Tissue healing changes on wounds in rats after treatment with Hancornia speciosa latex in cream-gel formulation

Bioactive glasses and electrospun composites that release cobalt to stimulate the HIF pathway for wound healing applications

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