Regional and ethnic differences in semen quality and reproductive hormones in Russia: A Siberian population‐based cohort study of young men

Осадчук, Л. В.; Шантанова, Л. Н.; I, Troev; Клещев, М. А.; Осадчук, А. В.

doi:10.1111/andr.13024

Cited by 15 publications

(16 citation statements)

References 64 publications

(237 reference statements)

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“…Physical Examination, Blood and Sperm Collection, Semen Analysis, and DNA Extraction Physical examination, blood and sperm collection, and semen analysis were described in detail earlier (Osadchuk et al, 2020(Osadchuk et al, , 2021. Briefly, all participants were examined by an experienced andrologist, who diagnosed urogenital disorders.…”

Section: Study Populationmentioning

confidence: 99%

Whole-Exome Sequencing Analysis of Human Semen Quality in Russian Multiethnic Population

et al. 2021

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The global trend toward the reduction of human spermatogenic function observed in many countries, including Russia, raised the problem of extensive screening and monitoring of male fertility and elucidation of its genetic and ethnic mechanisms. Recently, whole-exome sequencing (WES) was developed as a powerful tool for genetic analysis of complex traits. We present here the first Russian WES study for identification of new genes associated with semen quality. The experimental 3 × 2 design of the WES study was based on the analysis of 157 samples including three ethnic groups—Slavs (59), Buryats (n = 49), and Yakuts (n = 49), and two different semen quality groups—pathozoospermia (n = 95) and normospermia (n = 62). Additionally, our WES study group was negative for complete AZF microdeletions of the Y-chromosome. The normospermia group included men with normal sperm parameters in accordance with the WHO-recommended reference limit. The pathozoospermia group included men with impaired semen quality, namely, with any combined parameters of sperm concentration <15 × 106/ml, and/or progressive motility <32%, and/or normal morphology <4%. The WES was performed for all 157 samples. Subsequent calling and filtering of variants were carried out according to the GATK Best Practices recommendations. On the genotyping stage, the samples were combined into four cohorts: three sets corresponded to three ethnic groups, and the fourth set contained all the 157 whole-exome samples. Association of the obtained polymorphisms with semen quality parameters was investigated using the χ2 test. To prioritize the obtained variants associated with pathozoospermia, their effects were determined using Ensembl Variant Effect Predictor. Moreover, polymorphisms located in genes expressed in the testis were revealed based on the genomic annotation. As a result, the nine potential SNP markers rs6971091, rs557806, rs610308, rs556052, rs1289658, rs278981, rs1129172, rs12268007, and rs17228441 were selected for subsequent verification on our previously collected population sample (about 1,500 males). The selected variants located in seven genes FAM71F1, PPP1R15A, TRIM45, PRAME, RBM47, WDFY4, and FSIP2 that are expressed in the testis and play an important role in cell proliferation, meiosis, and apoptosis.

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Section: Study Populationmentioning

confidence: 99%

Whole-Exome Sequencing Analysis of Human Semen Quality in Russian Multiethnic Population

et al. 2021

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“…The recruitment protocol and physical examination methods had been described earlier in detail [56]. Briefly, male volunteers being interested in their reproductive health, with unknown fertility living in Novosibirsk (n = 278) and Kemerovo (n = 258) at least 5 years were enrolled in the study.…”

Section: Study Populationmentioning

confidence: 99%

“…The semen samples were collected by masturbation and analyzed for semen volume (ml), sperm concentration (mln/ml), progressive motility (percentage) and sperm morphology. Semen volume, sperm concentration and sperm count and progressive motility for all subjects were estimated according to the "WHO laboratory manual for the examination and processing of human semen" [2] as described earlier [56,57]. To study the relationships between impaired semen parameters (sperm concentration and sperm motility) and percentages of sperm morphological defects each participant was assigned to semen quality status (SQS) according to the sperm quality thresholds provided by the WHO [2]: normozoospermia (sperm concentration and percentage of progressively motile spermatozoa equal to or above the lower reference limits) and pathozoospermia (sperm concentration < 15 mln/ml and/or percentage of progressively motile spermatozoa<32%).…”

Section: Semen Samplesmentioning

confidence: 99%

“…For human populations of Western Siberia cross-sectional study of sperm morphological pattern and DNA fragmentation has not been performed also, although certain areas in this territory are characterized by severe environmental pollution due to metallurgical, chemical and mining enterprises. In particular, the Novosibirsk and Kemerovo cities are located to same climatic conditions but Kemerovsky region (Kuzbass) and the Kemerovo city are characterized by increased environmental pollution especially by PM due to coal mining and the numerous metallurgical and chemical industries [55,56]. In previous study it was shown that men from general populations of the Kemerovo city were characterized by lower sperm count, sperm motility, testosterone and inhibin B levels in comparison with the residents of Novosibirsk city [56].…”

Section: Introductionmentioning

confidence: 99%

“…In particular, the Novosibirsk and Kemerovo cities are located to same climatic conditions but Kemerovsky region (Kuzbass) and the Kemerovo city are characterized by increased environmental pollution especially by PM due to coal mining and the numerous metallurgical and chemical industries [55,56]. In previous study it was shown that men from general populations of the Kemerovo city were characterized by lower sperm count, sperm motility, testosterone and inhibin B levels in comparison with the residents of Novosibirsk city [56]. Investigation of sperm morphology pattern and DNA fragmentation in this region may be interesting for evaluating complex industrial pollution on sperm morphogenesis and chromatin compaction.…”

Section: Introductionmentioning

confidence: 99%

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Impaired semen quality, an increase of sperm morphological defects and DNA fragmentation associated with environmental pollution in urban population of young men from Western Siberia, Russia

Клещев

Осадчук

2021

PLoS ONE

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Poor sperm morphology and an elevated DNA fragmentation level are considered to be related to spermiogenesis malfunctions as a result of genetic mutations and effects of environmental factors, including industrial pollution. Standardized cross-sectional population studies of sperm morphology defects and sperm DNA fragmentation, especially in regions with increased environmental pollution may be helpful to investigate an influence of industrial pollution and other population-related factors on spermiogenesis process. The aim of present study was to estimate an influence industrial pollution on sperm morphogenesis and sperm DNA fragmentation in men from the general population of the Western Siberia. The Novosibirsk and Kemerovo cities are located to same climatic conditions in Western Siberia but the Kemerovo city is characterized by increased environmental pollution especially by particulate matter (PM). The male volunteers living in Novosibirsk (n = 278) and Kemerovo (n = 258) were enrolled. Percentages of sperm morphological defects are counted after staining native ejaculate smears by Diff-Quick kits. DNA fragmentation was estimated by a SCSA technique. The residents of Kemerovo were characterized by lowered sperm count and sperm motility, elevated DNA fragmentation, poor sperm morphology and increased incidence of morphological effects of head (pyriform, elongated, round, abnormal acrosome and vacuolated chromatine), asymmetrical neck insertion and excess residual cytoplasm. Moreover, elevated DNA fragmentation was associated with lowered sperm count, sperm motility and increased percentages of several sperm morphology defects, with the place of residence affecting the relationships between conventional semen parameters, sperm morphology and DNA fragmentations. Our study suggests that excessive sperm head elongation and impaired acrosome formation can contribute to sperm morphology deterioration in men from polluted areas. Regional features in the relationships between sperm morphology, sperm count and DNA fragmentation were shown, suggesting an importance of studying sperm morphology pattern in men from different regions.

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Association of Overweight and Obesity with Semen Parameters and Metabolic and Hormonal Status in Young Men

Osadchuk,

Kleshchev,

Osadchuk

2023

Bull Exp Biol Med

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Regional and ethnic differences in semen quality and reproductive hormones in Russia: A Siberian population‐based cohort study of young men

Cited by 15 publications

References 64 publications

Whole-Exome Sequencing Analysis of Human Semen Quality in Russian Multiethnic Population

Whole-Exome Sequencing Analysis of Human Semen Quality in Russian Multiethnic Population

Impaired semen quality, an increase of sperm morphological defects and DNA fragmentation associated with environmental pollution in urban population of young men from Western Siberia, Russia

Association of Overweight and Obesity with Semen Parameters and Metabolic and Hormonal Status in Young Men

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