Regionalization of pIgR expression in the mucosa of mouse small intestine

Nasal IgA secretion in a murine model of acute stress. The possible role of catecholamines.

“…RNA extraction, DNA synthesis and RT-PCR assays were all performed according to a previously reported method (Resendiz-Albor et al, 2010). Specific oligonucleotide primers were generated with the online assay design software (ProbeFinder: http://www.universalprobelibrary.com), using the primer sequence for each gene (Table 1).…”

Stress at the intestinal surface: catecholamines and mucosa–bacteria interactions

“…Glucocorticoids inhibit mucosal inflammation through activation of glucocorticoid receptors present in intestinal lymphocytes (Fujishima et al 2009). In addition, cytosolic glucocorticoid receptors are expressed in epithelial cells, where they may reduce the adverse effects of inflammatory mediators on epithelial barrier function (Boivin et al 2007) and immune function (Reséndiz-Albor et al 2010).…”

Intermittent fasting modulates IgA levels in the small intestine under intense stress: A mouse model

“…Intestinal fluids were stored at −70°C to await analysis of SIgA by ELISA assay. After washing, lamina propria cells from the two intestinal segments were purified by a Percoll gradient (Resendiz-Albor et al, 2010) to determine IgA+ plasma cells by flow cytometry (described below). Additionally, sections of each intestinal segment were scraped with a coverslip to collect samples of whole mucosa that were histologically evaluated.…”

“…At present, the modulation of intestinal IgA levels by intermittent fasting in mice under intense stress caused by a strenuous bout of exercise has not been analyzed in terms of intestinal regionalization that results from the differential distribution of immunocompetent cells and the pIgR as well as divergent sympathetic innervation (Tamura et al, 2003;Resendiz-Albor et al, 2010;Ke et al, 2011). Given the implications for homeostasis and protection of the gut, the aim of this study was to assess the impact of intermittent fasting on IgA levels and other mucosal parameters in response to intense stress caused by a final bout of forced swimming.…”