Registration of Tx772 Maize

Llorente, Carlos Fernando; Betrán, F. J.; Bockholt, A. J.; Fojt, F.

doi:10.2135/cropsci2004.1036a

Cited by 22 publications

(18 citation statements)

References 2 publications

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“…Tx906 (pedigree: Ethiopia15‐B‐5‐1‐B‐B2‐B‐1‐B‐B‐B) and a sister line of Tx903 (pedigree: {[(Lfy2361‐B/Tx114(B73w)‐BDarkblue‐B)Tx114]/Lfy2304‐B‐B}‐B‐1–3‐B‐B‐B‐3‐B‐B) were previously characterized for agronomic and phenolic acid content combining ability (Mahan et al, 2013), as well as for specific antioxidants and tortilla chip processing properties (Collison et al, 2015). Tx772 (Llorente et al, 2004) was chosen to incorporate aflatoxin resistance into the population. The fourth founding line, B73Olc1, is expected to be isogenic to the line from which the maize reference genome was drafted (Schnable et al, 2009) but has an ethyl methane sulfonate (EMS)‐induced mutation that nearly doubles the oleic acid content (Wright, 1995), providing another unique trait for further investigation.…”

Section: Methodsmentioning

confidence: 99%

Four‐Parent Maize (FPM) Population: Development and Phenotypic Characterization

2018

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To measure the effectiveness of multiparent population design in maize (Zea mays L.), an unprecedented, four‐parent maize (FPM) population was developed, incorporating a series of different mating designs. The FPM population incorporated up to three generations of intermating to allow for comparison of traditional biparental, multiparent, and multiparent intermated populations for phenotypic diversity. A total of 1291 inbred lines were evaluated per se with at least one replication among 5551 total progeny plots across two inbred trials 2013 and three inbred trials in 2014 at College Station, TX. These trials were phenotyped for days to anthesis and silking, plant and ear height, leaf rolling, and cob and kernel color (yellow endosperm, blue aleurone). Significant genetic variation was found for all traits analyzed, as well as substantial environmental variation for days to anthesis and days to silking. Overall, single replications of population entries performed well for agronomic trait analysis as indicated by low residual variation. Although mean flowering time did not show a particular relationship to specific subpopulations, greater variance was observed for plant height in some of the FPM populations than in the biparental populations. The creation of this population and the analysis of these traits serve to showcase the advantages and disadvantages of traditional and complex mating designs and reveal the potential to research agronomic and other traits within this population resource.

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Section: Methodsmentioning

confidence: 99%

Four‐Parent Maize (FPM) Population: Development and Phenotypic Characterization

2018

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“…Replicate samples of maize inbred line TX772 (Llorente et al, 2010) were grown in College Station in 2012 and subjected to one of three treatments: 1. Three ml of inoculum down the silk channel (Zummo and Scott, 1989).…”

Section: Inoculum Preparation and Applicationmentioning

confidence: 99%

“…When vitreous and floury endosperms were compared for protein and starch composition, the increase in alpha zeins (twice as much in flint compared to floury) as well as the arrangement and size of starch granules contributed to the hardness of the kernel (Gayral et al, 2016). Vitreous compared to softer dent type endosperm has been positively correlated with resistance to ear rot and aflatoxin contamination (Darrah et al, 1987;Betrán, Isakeit and Odvody, 2002;Llorente et al, 2010). Perhaps up-regulating zein genes as found at the blister stage in response to infection is one way that Tx772 builds up resistance to colonization by the fungus, as evidenced by greater expression of these genes in inoculated samples.…”

Section: Other Classes Of Genes De Not Directly Related To Pathogenicmentioning

confidence: 99%

“…TX772, a temperate Argentinian inbred featuring a hard and vitreous endosperm (Betrán, Isakeit and Odvody, 2002;Llorente et al, 2010) does not share a common pedigree with most U.S. lines of Reid Yellow Dent, Lancaster or Iodent types (Goodman, 2005), and is genetically distant based on existing marker data (Smith, Murray and Heffner, 2015). Hybrids of Tx772 with BSSS germplasm (Stiff Stalk Synthetic) have exhibited high yield potential under irrigation.…”

Section: Introductionmentioning

confidence: 99%

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Maize Kernel Development Stage the Primary Factor in Differential Gene Expression in Response to Two Methods of Field Inoculation withAspergillus flavus

Wahl

Murray

Zhang

et al. 2019

Preprint

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Title: Maize Kernel Development Stage the Primary Factor in Differential Gene Expression inResponse to Two methods of Field Inoculation with Aspergillus flavus Background: Aflatoxins, produced by the fungus Aspergillus flavus, often contaminate preharvest maize (Zea mays L.) grain under heat and drought stresses, posing serious health hazards to humans and livestock, and resulting in significant costs to identify and dispose of contaminated grain. This study was designed to investigate the changes in differential gene expression during seed morphogenesis and maturation in the "aflatoxin resistant" Argentinian inbred line Tx772 when challenged by the introduction of A. flavus through two different methods of ear inoculation; non-wounding (silk channel, used to select Tx772), wounding (side needle) and a non-inoculated control. Method and Findings:Grain maturity had the largest effect on overall RNA-Seq differential gene expression (DGE) as measured by edgeR of the Bioconductor platform. However, within each stage of development, ranging from blister to dent, similar up-regulation in expression of many maize genes following inoculation with either method was observed; a total of 16 genes previously associated with resistance to pathogens were identified among the transcripts differentially expressed (DE) at p ≤ .05, FDR ≤ .10, and fold change ≥ 2.0 over all stages. The side needle technique produced a larger effect of infection as evidenced by 6,324 fungal reads versus 518 in silk channel and a higher level of aflatoxin. Correlations between approximately 7,000 fungal reads and the number of maize DE genes for each of the eight treatment groups was 0.56 (p = .152) and was 0.65 (p < .001) with levels of aflatoxin ranging from 0 to 137 ng g-1.3 Conclusion: These correlations provided an internal measure of effectiveness of inoculation methods that were associated with the mostly up-regulation of defense-related genes in response to the presence of Aspergillus flavus in a unique maize genetic background.

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“…Early breeding efforts identified A. flavusresistant inbred lines Mp420 and Mp313E Zummo, 1990, 1992). Additional germplasm lines developed for resistance to aflatoxin accumulation included GT-601-GT-603 developed from the GT-MAS:gk population, and Mp715, Mp717-Mp719, Tx736, Tx739, Tx740, Tx772, TZAR101-TZAR106 (McMillian et al, 1993;Williams and Windham, 2001Llorente et al, 2004;Guo et al, 2007Guo et al, , 2011Menkir et al, 2008;Mayfield et al, 2012). Despite steady development of genetic sources of resistance to aflatoxin accumulation in maize germplasm, attempts to transfer resistance to more agronomically adequate hybrids have been challenging (Abbas et al, 2002;Warburton and Williams, 2014).…”

Section: Introductionmentioning

confidence: 99%

Mapping Quantitative Trait Loci Associated With Resistance to Aflatoxin Accumulation in Maize Inbred Mp719

et al. 2020

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Aflatoxins are carcinogenic and toxic compounds produced principally by fungal species Aspergillus flavus (Link: Fries) and A. parasiticus (Speare), which are common contaminants of food and feed. Aflatoxins can be found at dangerously high levels and can readily contaminate pre-harvest maize (Zea mays L.) grain. Sources of resistance to aflatoxin accumulation in maize have been identified, however, the highly quantitative nature and complex inheritance of this trait have limited the introgression of aflatoxin accumulation resistance into agronomically desirable lines. Mapping of quantitative trait loci (QTL) was performed on a bi-parental population comprised of 241 F2:3 families derived from the cross of inbred lines Mp705 (susceptible) × Mp719 (resistant). The mapping population was phenotyped in replicated field trials in three environments for resistance to aflatoxin accumulation under artificial inoculation with an A. flavus spore suspension. The genetic linkage map was constructed with 1,276 single nucleotide polymorphism (SNP) and simple sequence repeat (SSR) molecular markers covering a total genetic distance of 1,642 cM across all ten maize chromosomes. Multiple interval mapping revealed that majority of the aflatoxin-reducing alleles and the source for the larger effect QTL identified in this study were contributed from Mp719, the resistant parent. Two QTL identified on chromosome 1 (bin 1.06-1.07) and chromosome 3 (bin 3.09) were the most stable across different environments and when combined, explained 24.6% of the total phenotypic variance across all three environments. Results from the study showed that these chromosomal regions harbor important QTL for influencing aflatoxin accumulation, which is consistent with previous reports with other different mapping populations. These stable QTL were the most promising for controlling aflatoxin accumulation in maize grain. Identifying beneficial alleles derived from Mp719 and closely linked molecular markers through QTL analysis for implementation of MAS could accelerate breeding efforts to reduce aflatoxin accumulation in maize.

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Registration of Tx772 Maize

Cited by 22 publications

References 2 publications

Four‐Parent Maize (FPM) Population: Development and Phenotypic Characterization

Four‐Parent Maize (FPM) Population: Development and Phenotypic Characterization

Maize Kernel Development Stage the Primary Factor in Differential Gene Expression in Response to Two Methods of Field Inoculation withAspergillus flavus

Mapping Quantitative Trait Loci Associated With Resistance to Aflatoxin Accumulation in Maize Inbred Mp719

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