Registration of Zak <i>ERA8</i>
 Soft White Spring Wheat Germplasm with Enhanced Response to ABA and Increased Seed Dormancy

Martinez, Shantel A.; Schramm, Elizabeth C.; Harris, Tracy J.; Kidwell, K. K.; Garland‐Campbell, Kimberly; Steber, Camille M.

doi:10.3198/jpr2013.09.0060crg

Cited by 4 publications

(9 citation statements)

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“…S1). ERA8 segregates as a single semidominant Mendelian trait in backcross populations when the phenotype is examined at optimized ABA concentrations and after-ripening time points (Schramm et al 2013;Martinez et al 2014). Consistent with this, the Zak/ZakERA8 BC 3 F 2 population (X5.2) showed segregation as a single semi-dominant gene on 2 μ M ABA at 5 weeks of after-ripening (χ2 = 1.62, p-value = 0.20; Table S2).…”

Section: Era8 Mapping By Bulked Segregant Analysis Of a Backcross Popsupporting

confidence: 62%

“…Moreover, mapping in a backcross population simplifies the genetics, decreasing the impact of other loci. BSA of the backcross population had the advantage that the ABA-hypersensitive phenotype showed Mendelian segregation because ERA8 was the major locus contributing the ABA-hypersensitive phenotype (Table S2; Table S5; Martinez et al 2014). While Louise was selected as an RIL population parent based on having dormancy characteristics similar to Zak, we were blind to the precise genetic alleles contributing to the dormancy or lack of dormancy in Louise (Fig.…”

Section: Comparison Of Era8 Mapping By Qtl Analysis and Bsa-exome-seqmentioning

confidence: 99%

“…The ERA mutants were identified by screening for increased sensitivity to inhibition of seed germination by ABA. The 'ZakERA8' line has strong ABA sensitivity and seed dormancy that is lost rapidly through after-ripening (Schramm et al 2013;Martinez et al 2014Martinez et al , 2016. Moreover, ZakERA8 had higher PHS tolerance in spike-wetting tests (Martinez et al 2016).…”

Section: Introductionmentioning

confidence: 99%

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Exome sequencing of bulked segregants identified a novel TaMKK3-A allele linked to the wheat ERA8 ABA-hypersensitive germination phenotype

Martinez

Shorinola

Conselman

et al. 2019

Preprint

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Author contribution statement: SAM, OS, CMS, and CU designed the experiments. SAM and CMS developed the populations. SAM and SC performed the Louise/ZakERA8 QTL analysis. DZS and DS conducted GBS and analyzed the raw sequence data. SAM performed germination assays for the bulked segregant analysis, fine mapped ERA8, and introgressed ERA8 into breeding lines. OS performed the exome capture, BSA, and designed KASP markers. SAM and OS conducted the RNA sequencing and TaMKK3-A alignment. CMS and CU provided resources. SAM and CMS wrote the manuscript. All authors read and approved the final submission.Abstract: Preharvest sprouting (PHS) is the germination of mature grain on the mother plant when it rains before harvest. The ENHANCED RESPONSE TO ABA8 (ERA8) mutant increases seed dormancy and, consequently, PHS tolerance in soft white wheat 'Zak'. ERA8 was mapped to chromosome 4A in a Zak/'ZakERA8' backcross population using bulked segregant analysis of exome sequenced DNA (BSA-exome-seq). ERA8 was fine-mapped relative to mutagen-induced SNPs to a 4.6 Mb region containing 70 genes. In the backcross population, the ERA8 ABA hypersensitive phenotype was strongly linked to a missense mutation TaMKK3-A-G1093A (LOD 16.5), a gene associated with natural PHS tolerance in barley and wheat. The map position of ERA8 was confirmed in an 'Otis'/ZakERA8 but not in a 'Louise'/ZakERA8 mapping population. This is likely because Otis carries the same natural PHS susceptible MKK3-A-A660 S allele as Zak, whereas Louise carries the PHS tolerant MKK3-A-C660 R allele. Thus, the variation for grain dormancy and PHS tolerance in the Louise/ZakERA8 population likely resulted from segregation of other loci rather than segregation for PHS tolerance at the MKK3 locus. This inadvertent complementation test suggests that the MKK3-A-G1093A mutation causes the ERA8 phenotype. Moreover, MKK3 was a known ABA signaling gene in the 70-gene 4.6 Mb ERA8 interval. None of these 70 genes showed the differential regulation in wild-type Zak versus ERA8 expected of a promoter mutation. Thus, the working model is that the ERA8 phenotype results from the MKK3-A-G1093A mutation. Key MessageUsing bulked segregant analysis of exome sequence, we fine-mapped the ABA hypersensitive mutant ERA8 in a wheat backcross population to the TaMKK3-A locus of chromosome 4A.

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Section: Era8 Mapping By Bulked Segregant Analysis Of a Backcross Popsupporting

confidence: 62%

Section: Comparison Of Era8 Mapping By Qtl Analysis and Bsa-exome-seqmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exome sequencing of bulked segregants identified a novel TaMKK3-A allele linked to the wheat ERA8 ABA-hypersensitive germination phenotype

Martinez

Shorinola

Conselman

et al. 2019

Preprint

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“…Allohexaploid white spring wheat cultivars (Triticum aestivum L.) were used in this study. 'ZakERA8' (PI 669443) is derived from the soft white spring cultivar 'Zak' (PI 612956) via an EMS-induced mutation resulting in enhanced response to ABA during seed germination (Kidwell et al 2002;Schramm et al 2013;Martinez et al 2014). Soft white spring 'Louise' (PI 634865) was selected from the cross 'Wakanz'/'Wawawai' (Kidwell et al 2006b).…”

Section: Plant Materialsmentioning

confidence: 99%

“…The 'ZakERA8′ line has increased seed dormancy, seed ABA sensitivity, and PHS tolerance than wild-type Zak (Schramm et al 2013;Martinez et al 2014Martinez et al , 2016. Since ERA8 dormancy is lost rapidly through 8-10 weeks of after-ripening, this mutation does not have an obvious adverse effect on emergence (Martinez et al 2014(Martinez et al , 2016. Thus, ERA8 is a good allele for improving PHS tolerance.…”

Section: Introductionmentioning

confidence: 99%

Exome sequencing of bulked segregants identified a novel TaMKK3-A allele linked to the wheat ERA8 ABA-hypersensitive germination phenotype

et al. 2020

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Key message Using bulked segregant analysis of exome sequence, we fine-mapped the ABA-hypersensitive mutant ERA8 in a wheat backcross population to the TaMKK3-A locus of chromosome 4A. Abstract Preharvest sprouting (PHS) is the germination of mature grain on the mother plant when it rains before harvest. The ENHANCED RESPONSE TO ABA8 (ERA8) mutant increases seed dormancy and, consequently, PHS tolerance in soft white wheat 'Zak.' ERA8 was mapped to chromosome 4A in a Zak/'ZakERA8' backcross population using bulked segregant analysis of exome sequenced DNA (BSA-exome-seq). ERA8 was fine-mapped relative to mutagen-induced SNPs to a 4.6 Mb region containing 70 genes. In the backcross population, the ERA8 ABA-hypersensitive phenotype was strongly linked to a missense mutation in TaMKK3-A-G1093A (LOD 16.5), a gene associated with natural PHS tolerance in barley and wheat. The map position of ERA8 was confirmed in an 'Otis'/ZakERA8 but not in a 'Louise'/ZakERA8 mapping population. This is likely because Otis carries the same natural PHS susceptible MKK3-A-A660 S allele as Zak, whereas Louise carries the PHStolerant MKK3-A-C660 R allele. Thus, the variation for grain dormancy and PHS tolerance in the Louise/ZakERA8 population likely resulted from segregation of other loci rather than segregation for PHS tolerance at the MKK3 locus. This inadvertent complementation test suggests that the MKK3-A-G1093A mutation causes the ERA8 phenotype. Moreover, MKK3 was a known ABA signaling gene in the 70-gene 4.6 Mb ERA8 interval. None of these 70 genes showed the differential regulation in wild-type Zak versus ERA8 expected of a promoter mutation. Thus, the working model is that the ERA8 phenotype results from the MKK3-A-G1093A mutation.

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The wheat ABA hypersensitive ERA8 mutant is associated with increased preharvest sprouting tolerance and altered hormone accumulation

et al. 2016

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Registration of Zak ERA8 Soft White Spring Wheat Germplasm with Enhanced Response to ABA and Increased Seed Dormancy

Cited by 4 publications

References 14 publications

Exome sequencing of bulked segregants identified a novel TaMKK3-A allele linked to the wheat ERA8 ABA-hypersensitive germination phenotype

Exome sequencing of bulked segregants identified a novel TaMKK3-A allele linked to the wheat ERA8 ABA-hypersensitive germination phenotype

Exome sequencing of bulked segregants identified a novel TaMKK3-A allele linked to the wheat ERA8 ABA-hypersensitive germination phenotype

The wheat ABA hypersensitive ERA8 mutant is associated with increased preharvest sprouting tolerance and altered hormone accumulation

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