Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analysis

Moreno, Armando Quintero; Rigau, Teresa; Rodríguez‐Gil, Joan E.

doi:10.1016/s0093-691x(03)00248-6

Cited by 126 publications

(104 citation statements)

References 27 publications

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“…centrifugation) of a given sperm suspension (Amann & Katz 2004). A more objective determination of motility is important since this parameter can be considered like a functional test, as the exact motion parameter that is shown by a cell is a direct determination of the energy status of this sperm (Quintero-Moreno et al 2004). As a result, the CASA-subjected study of motility can be included in the group of functional tests that can be considered as useful for in vivo predictive evaluation of an ejaculate (Quintero-Moreno et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…A more objective determination of motility is important since this parameter can be considered like a functional test, as the exact motion parameter that is shown by a cell is a direct determination of the energy status of this sperm (Quintero-Moreno et al 2004). As a result, the CASA-subjected study of motility can be included in the group of functional tests that can be considered as useful for in vivo predictive evaluation of an ejaculate (Quintero-Moreno et al 2004). In this sense, our mean values obtained in boar sperm motion parameters incubated in control conditions, noncapacitation medium (TBM) for 1 h at 398C (Table 2), are within the range obtained by others authors (Holt et al 1996, Abaigar et al 1999, Quintero-Moreno et al 2004, which supports the use of this experimental approach to study the possible involvement of the PI3-K intracellular pathway in the regulation of motility of boar spermatozoa.…”

Section: Discussionmentioning

confidence: 99%

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Inhibition of phosphatidylinositol 3-kinase modifies boar sperm motion parameters

Aparicio¹,

Gil²,

García-Herreros³

et al. 2005

Reproduction

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Motility is the most widely used indicator of sperm quality. Besides modulation by the cAMP pathway little is known regarding the intracellular pathways that regulate boar sperm motility. Recently the role of phosphatidylinositol 3-kinase (PI3-K) in the regulation of human sperm motility has been described. Therefore, the aim of this study was to investigate the role of PI3-K in boar sperm kinematics by using the specific PI3-K inhibitor, LY294002. Boar sperm was incubated up to 1 h in non-capacitating medium in the presence or absence of the cAMP analog, 8Br-cAMP or the PI3-K inhibitor, LY294002 or both. Boar sperm incubated in capacitating medium was treated in the presence or absence of LY294002. First, we have clearly identified that PI3-K is present in whole lysates of boar spermatozoa. Inhibition of PI3-K significantly increased boar sperm straight-line velocity, circular velocity and average velocity without an effect on the percentage of progressively motile spermatozoa in both media. Inhibition of PI3-K induced the same effects on boar sperm velocities as activation of the cAMP/protein kinase A (PKA) pathway and treatment with the PI3-K inhibitor, LY294002 had neither summatory nor synergic effects on boar sperm motion parameters when treated simultaneously with the cAMP analog 8Br-cAMP. Our data suggest that PI3-K plays a negative role, regulating boar sperm motion parameters through a possible inhibition of the cAMP/PKA activating pathway, and since some Computer Aided Sperm Analysis (CASA)-derived parameters have been related to field fertility our results point to the possibility of modulating sperm motile quality by modifying the PI3-K cellular pathway.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Inhibition of phosphatidylinositol 3-kinase modifies boar sperm motion parameters

Aparicio¹,

Gil²,

García-Herreros³

et al. 2005

Reproduction

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“…Several studies have highlighted, in the raw ejaculate, the presence of different subpopulations classified based on specific characteristics, such as morphology (NunezMartinez et al 2005), osmotic resistance (Perez-Llano et al 2003, Quintero-Moreno et al 2004, and acrosome status (Perez-Llano et al 2003). However, the sperm kinetic parameters are the most-used features to identify sperm subpopulations in humans (Chantler et al 2004) and animals (Holt et al 1996, Abaigar et al 1999, 2001, Quintero-Moreno et al 2003, MartinezPastor et al 2005a, Nunez-Martinez et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat

et al. 2012

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This study was designed for the identification of different sperm kinetic subpopulations in feline semen using artificial neural networks (ANNs) and for the evaluation of the effect of ejaculation on motility patterns of these subpopulations. Seven tomcats presented for routine orchiectomy were electroejaculated, and after 5 days, orchiectomized and epididymal tail sperms were collected. Sperm motility characteristics were evaluated using a computer-assisted sperm analyzer that provided individual kinetic characteristics of each spermatozoon. A total of 23 400 spermatozoa for electroejaculated and 9200 for epididymal tail samples were evaluated using a multivariate approach, comprising principal component analysis and ANN classification. The multivariate approach allowed the identification and characterization of three different and well-defined sperm subpopulations. There were significant differences before (epididymal tail spermatozoa) and after (electroejaculated sperm) ejaculation in sperm kinetic subpopulation characteristics. In both epididymal and ejaculated samples, the majority of subpopulation was characterized by high velocity and progressiveness; however, the electroejaculated samples showed significantly higher values, suggesting that the microenvironment of the epididymal tail could affect the sperm motility or, alternatively, seminal plasma could increase the kinetic characteristics of the spermatozoa, indicating that only after ejaculation, the spermatozoa express their motility potential. Nevertheless, further studies are required to clarify the functional significance of each kinetic subpopulation.

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“…The existence of well-defined motile sperm subpopulations within mammalian ejaculates is now widely accepted by the scientific community [8][9][10][11][12][13][14]. In several species, semen cryopreservation has been shown to modify the different subpopulations of motile sperm originally present in the fresh ejaculates, and such modifications can affect either the motility parameters defining each subpopulation or the frequency distribution of spermatozoa within them [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Effects of cryopreservation on the motile sperm subpopulations in semen from Asturiana de los Valles bulls

et al. 2009

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The aim of this study was to identify different motile sperm subpopulations in ejaculates from an autochthonous bull breed (Bos taurus) and to determine possible modifications in these subpopulations resulting from cryopreservation. Ejaculates were collected and cryopreserved following a conventional protocol. The overall sperm motility and the kinematic parameters of individual spermatozoa were evaluated in fresh ejaculates, after 4 h at 5 8C, and at 0 and 2 h postthaw. A multivariate clustering procedure separated 23,585 motile spermatozoa into four subpopulations: Subpopulation 1 showed medium velocity (VCL: 99.4 AE 17.8 mm/sec) and high progressiveness (LIN: 65.1 AE 14.0%); Subpopulation 2 included spermatozoa with high velocity (VCL: 148.7 AE 25.6 mm/sec) but a nonprogressive trajectory (LIN: 33.1 AE 10.5%); Subpopulation 3 represented slowly motile (VCL: 58.3 AE 24.3 mm/sec) and nonprogressive sperm (LIN: 39.6 AE 18.3%); and Subpopulation 4 included very rapid (VCL: 152.8 AE 25.7 mm/sec) and highly progressive sperm (LIN: 70.9 AE 13.7%). Subpopulation 4 was present in the greatest quantity in fresh ejaculates (36%), but after cooling, it significantly decreased (21%) concomitantly with an increase (P < 0.001) in Subpopulation 2 (from 21% in fresh to 34% in postcooled semen). After freezing and thawing, the overall sperm motility was reduced, mainly due to Subpopulation 2 decreasing from 34% after cooling to 14% after thawing. Differences among bulls in the frequency distribution of spermatozoa within subpopulations were evidenced after thawing by different proportions of spermatozoa in Subpopulations 2 and 4. The current results indicate that a structure of four sperm subpopulations may be a common characteristic of bovine ejaculates and that the cooling phase of cryopreservation seems to be the determinant of postthaw semen quality. #

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Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analysis

Cited by 126 publications

References 27 publications

Inhibition of phosphatidylinositol 3-kinase modifies boar sperm motion parameters

Inhibition of phosphatidylinositol 3-kinase modifies boar sperm motion parameters

Artificial neural networks for the definition of kinetic subpopulations in electroejaculated and epididymal spermatozoa in the domestic cat

Effects of cryopreservation on the motile sperm subpopulations in semen from Asturiana de los Valles bulls

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