Regression Analysis in Development of Procedure for Isolation and Quantitative Deter-Mination of Water-Soluble Polysaccharides From Dandelion Roots of Medicinal

Abstract

The roots of the dandelion drug (Taraxacum officinale F.H. Wigg) are rich in chemical composition and contain water-soluble polysaccharides based on inulin. The aim of the study is to select optimal parameters of ultrasonic extraction of water-soluble polysaccharides from dandelion roots based on regression analysis. For the experiment, raw materials purchased from one of the pharmacies in the city of Voronezh were used. A sample of raw materials (1.0 g) was placed in an ultrasonic bath "Grad 40-35," which allows maintaining the necessary temperature and frequency of ultrasound with a given duration of time. one-year old, as well as in industrial production of water-soluble polysaccharides from this kind of raw material. Then, 10 (15 or 20) ml of purified water purified from impurities at a temperature of 60 °C to 80 °C was measured with a measuring flask and extracted from 10 to 20 min with a multiplicity of 1 to 3, and after each of the multiple studies, the same raw material was further filtered with a new solvent. The initial conditions and the data obtained during the experiments made it possible to present the regression model in the form of a linear multiple regression equation, which can be used with sufficient accuracy in prediction and analysis. The adequacy of the model was confirmed by testing hypotheses against Pearson's x2 criterion. The correlation coefficient derived from the values of the regression coefficient and the mean square deviation was used in determining the tightness of the overall relationship between the source and the obtained data. In addition, a detailed study of a sample of those variables whose priority was not clearly expressed was carried out by processing when fixing their values. The most important conditions for extraction of dandelion roots for obtaining the most stable performance should be considered: extraction temperature – 80 °C; extraction multiplicity – 3, ultrasound frequency – 35 kHz, extraction time – 40 min, ratio of raw material and extractant – 1 g per 10 ml, at the same time, grinding of raw material can vary from 0.2 mm to 0.5 mm.